Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/57928
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dc.contributor.authorMilne, Douglas Johnen_US
dc.contributor.authorFernández Montero, Álvaroen_US
dc.contributor.authorGundappa, Manu K.en_US
dc.contributor.authorWang, Tiehuien_US
dc.contributor.authorAcosta, Félixen_US
dc.contributor.authorTorrecillas Burriel,Silviaen_US
dc.contributor.authorMontero, Danielen_US
dc.contributor.authorZou, Junen_US
dc.contributor.authorSweetman, Johnen_US
dc.contributor.authorSecombes, Christopher Johnen_US
dc.date.accessioned2019-11-18T09:24:04Z-
dc.date.available2019-11-18T09:24:04Z-
dc.date.issued2019en_US
dc.identifier.issn0161-5890en_US
dc.identifier.urihttp://hdl.handle.net/10553/57928-
dc.description.abstractAntimicrobial peptides (AMPs) play an important role in the innate immune response of vertebrates by creating a hostile environment for any invading pathogens. Piscidins are potent teleost specific AMPs, which have a broad spectrum activity. We have identified a novel piscidin active peptide, in the greater amberjack, Seriola dumerili, that consists of 25 aa, which forms an amphipathic helix with distinct hydrophobic and positively charged regions. Following homology and phylogenetic analysis the greater amberjack piscidin was deemed to belong to the group 3 family of piscidins. Piscidin was expressed constitutively at immune sites, with transcript level highest in the spleen and gut, at an intermediate level in the gills and lowest in the head kidney. Following in vivo stimulation with PAMPs (poly I:C, LPS and flagellin) piscidin transcript level increased in gills in response to flagellin, in gut and spleen in response to poly I:C, and in head kidney in response to poly I:C, LPS and flagellin. Head kidney and spleen cells were then isolated from greater amberjack and incubated with each of the PAMPs for 4, 12 and 24 h. Piscidin expression was unchanged at 4 and 12 h post PAMP stimulation in head kidney cells but at 24 h transcript level was markedly upregulated compared to control (unstimulated) cells, especially with the bacterial PAMPs. In contrast, spleen cells upregulated piscidin expression by 4 h post stimulation with poly I:C and flagellin, and remained upregulated to 24 h with flagellin exposure, but had returned to baseline levels by 12 h using poly I:C. To determine if piscidin expression could be modulated by diet, greater amberjack were fed diets supplemented with MOS and cMOS for 30 days when transcript level was determined. It was found that MOS supplemented diets increased expression in the spleen, cMOS supplemented diets upregulated transcript levels in the gills and head kidney, whilst a diet containing both MOS and cMOS upregulated transcript in the gut, when compared to fish fed the control diet. Finally, a synthetic greater amberjack piscidin was produced and showed bacteriostatic activity against a number of bacterial strains, including both Gram positive and Gram negative fish pathogens.en_US
dc.languageengen_US
dc.relation.ispartofMolecular Immunologyen_US
dc.sourceMolecular Immunology [ISSN 0161-5890], v. 114, p. 378-388en_US
dc.subject251092 Acuicultura marinaen_US
dc.subject.otherGreater amberjacken_US
dc.subject.otherPiscidinen_US
dc.subject.otherInnate immunityen_US
dc.subject.otherPAMPen_US
dc.subject.otherBacteriostatic activityen_US
dc.titleAn insight into piscidins: The discovery, modulation and bioactivity of greater amberjack, Seriola dumerili, piscidinen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.molimm.2019.08.005
dc.identifier.scopus85070961929
dc.identifier.isi000490625600044
dc.contributor.authorscopusid57192718956
dc.contributor.authorscopusid57200550839
dc.contributor.authorscopusid57194542165
dc.contributor.authorscopusid8785208500
dc.contributor.authorscopusid56269311600
dc.contributor.authorscopusid21733894700
dc.contributor.authorscopusid35605929400
dc.contributor.authorscopusid57042236300
dc.contributor.authorscopusid34882169900
dc.contributor.authorscopusid7005974493
dc.description.lastpage388-
dc.description.firstpage378-
dc.relation.volume114-
dc.investigacionCienciasen_US
dc.type2Artículoen_US
dc.contributor.daisngid3736331
dc.contributor.daisngid31509276
dc.contributor.daisngid20172688
dc.contributor.daisngid493937
dc.contributor.daisngid878226
dc.contributor.daisngid1545065
dc.contributor.daisngid432360
dc.contributor.daisngid431287
dc.contributor.daisngid685263
dc.contributor.daisngid34998
dc.contributor.wosstandardWOS:Milne, DJ
dc.contributor.wosstandardWOS:Fernandez-Montero, A
dc.contributor.wosstandardWOS:Gundappa, MK
dc.contributor.wosstandardWOS:Wang, TH
dc.contributor.wosstandardWOS:Acosta, F
dc.contributor.wosstandardWOS:Torrecillas, S
dc.contributor.wosstandardWOS:Montero, D
dc.contributor.wosstandardWOS:Zou, J
dc.contributor.wosstandardWOS:Sweetman, J
dc.contributor.wosstandardWOS:Secombes, CJ
dc.date.coverdateOctubre 2019
dc.identifier.ulpgces
dc.description.sjr1,092
dc.description.jcr3,641
dc.description.sjrqQ2
dc.description.jcrqQ2
dc.description.scieSCIE
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR Grupo de Investigación en Acuicultura-
crisitem.author.deptIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR Grupo de Investigación en Acuicultura-
crisitem.author.deptIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.deptGIR Grupo de Investigación en Acuicultura-
crisitem.author.deptIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.deptDepartamento de Biología-
crisitem.author.orcid0000-0002-1098-7529-
crisitem.author.orcid0000-0002-4358-2157-
crisitem.author.parentorgIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.parentorgIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.parentorgIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.fullNameFernández Montero, Álvaro-
crisitem.author.fullNameAcosta Arbelo, Félix Antonio-
crisitem.author.fullNameTorrecillas Burriel, Silvia-
crisitem.author.fullNameMontero Vítores, Daniel-
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