Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/55078
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dc.contributor.authorBernhard, Annetteen_US
dc.contributor.authorRasinger, Josef D.en_US
dc.contributor.authorBetancor, Monica B.en_US
dc.contributor.authorCaballero, Maria Joséen_US
dc.contributor.authorBerntssen, Marc H.G.en_US
dc.contributor.authorLundebye, Anne Katrineen_US
dc.contributor.authorØrnsrud, Robinen_US
dc.date.accessioned2019-02-18T16:25:22Z-
dc.date.available2019-02-18T16:25:22Z-
dc.date.issued2019en_US
dc.identifier.issn1932-6203en_US
dc.identifier.otherWoS-
dc.identifier.urihttp://hdl.handle.net/10553/55078-
dc.description.abstractEthoxyquin (EQ; 6-Ethoxy-2,2,4-trimethyl-1,2-dihydroquinoline) has been used as an antioxidant in feed components for pets, livestock and aquaculture. However, possible risks of EQ used in aquafeed for fish health have not yet been characterized. The present study investigated the toxicity and dose-response of subchronic dietary EQ exposure at doses ranging from 41 to 9666 mg EQ/kg feed in Atlantic salmon (Salmo salar L.). Feed at concentrations higher than 1173 mg EQ/kg were rejected by the fish, resulting in reduced feed intake and growth performance. No mortality was observed in fish exposed to any of the doses. A multi-omic screening of metabolome and proteome in salmon liver indicated an effect of dietary EQ on bioenergetics pathways and hepatic redox homeostasis in fish fed concentrations above 119 mg EQ/kg feed. Increased energy expenditure associated with an upregulation of hepatic fatty acid beta-oxidation and induction and carbohydrate catabolic pathways resulted in a dose- dependent depletion of intracytoplasmic lipid vacuoles in liver histological sections, decreasing whole body lipid levels and altered purine/pyrimidine metabolism. Increased GSH and TBARS in the liver indicated a state of oxidative stress, which was associated with activation of the NRF2-mediated oxidative stress response and glutathione-mediated detoxification processes. However, no oxidative DNA damage was observed. As manifestation of altered energy metabolism, the depletion of liver intracytoplasmic lipid vacuoles was considered the critical endpoint for benchmark dose assessment, and a BMDL10 of 243 mg EQ/kg feed was derived as a safe upper limit of EQ exposure in Atlantic salmon.en_US
dc.languageengen_US
dc.relation.ispartofPLoS ONEen_US
dc.sourcePlos One [ISSN 1932-6203], v. 14 (1), (Enero 2019)en_US
dc.subject.otherSynthetic Antioxidant Ethoxyquinen_US
dc.subject.otherFed Graded-Levelsen_US
dc.subject.otherDna-Damageen_US
dc.subject.otherButylated Hydroxyanisoleen_US
dc.subject.otherOxidative Stressen_US
dc.subject.otherGlutathioneen_US
dc.subject.otherMetabolismen_US
dc.subject.otherExpressionen_US
dc.subject.otherGrowthen_US
dc.subject.otherBiotransformationen_US
dc.titleTolerance and dose-response assessment of subchronic dietary ethoxyquin exposure in Atlantic salmon (Salmo salar L.)en_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0211128en_US
dc.identifier.scopus85060521035-
dc.identifier.isi000457037500104-
dc.contributor.authorscopusid36930033900-
dc.contributor.authorscopusid55929492000-
dc.contributor.authorscopusid26431740800-
dc.contributor.authorscopusid57209992208-
dc.contributor.authorscopusid6603855136-
dc.contributor.authorscopusid6701639022-
dc.contributor.authorscopusid56117716100-
dc.identifier.issuee0211128-
dc.relation.volume14en_US
dc.investigacionCienciasen_US
dc.type2Artículoen_US
dc.contributor.daisngid30362923-
dc.contributor.daisngid1685246-
dc.contributor.daisngid832465-
dc.contributor.daisngid436538-
dc.contributor.daisngid369981-
dc.contributor.daisngid359631-
dc.contributor.daisngid1361121-
dc.description.numberofpages36en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Bernhard, A-
dc.contributor.wosstandardWOS:Rasinger, JD-
dc.contributor.wosstandardWOS:Betancor, MB-
dc.contributor.wosstandardWOS:Caballero, MJ-
dc.contributor.wosstandardWOS:Berntssen, MHG-
dc.contributor.wosstandardWOS:Lundebye, AK-
dc.contributor.wosstandardWOS:Ornsrud, R-
dc.date.coverdateEnero 2019en_US
dc.identifier.ulpgces
dc.description.sjr1,1
dc.description.jcr2,776
dc.description.sjrqQ1
dc.description.jcrqQ2
dc.description.scieSCIE
dc.description.erihplusERIH PLUS
item.grantfulltextopen-
item.fulltextCon texto completo-
crisitem.author.deptGIR IUSA-ONEHEALTH 3: Histología y Patología Veterinaria y Forense (Terrestre y Marina)-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Morfología-
crisitem.author.orcid0000-0002-2575-0997-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameCaballero Cansino, María José-
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