Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/55012
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dc.contributor.authorSaavedra, Ester
dc.contributor.authorDel Rosario, Henoc
dc.contributor.authorBrouard, Ignacio
dc.contributor.authorQuintana, José
dc.contributor.authorEstévez, Francisco
dc.date.accessioned2019-02-18T16:08:11Z-
dc.date.available2019-02-18T16:08:11Z-
dc.date.issued2019
dc.identifier.issn0009-2797
dc.identifier.urihttp://hdl.handle.net/10553/55012-
dc.description.abstractIn this study, we investigated the effects of synthetic 6'-benzyloxy-4-bromo-2'-hydroxychalcone on viabilities of seven human leukaemia cells. It was cytotoxic against U-937, HL-60, K-562, NALM-6, MOLT-3 cells, and also against Bcl-2-overexpressing U-937/Bcl-2 cells and P-glycoprotein-overexpressing K-562/ADR, but had no significant cytotoxic effects against quiescent or proliferating human peripheral blood mononuclear cells. This chalcone is a potent apoptotic inducer in human leukaemia U-937 cells. Cell death was (i) mediated by the activation and the cleavage of initiator and executioner caspases and poly(ADP-ribose) polymerase; (ii) prevented by the pan-caspase inhibitor z-VAD-fmk, and by the selective caspase-3/7, -6 and -8 inhibitors, and by a cathepsins B/L inhibitor; (iii) associated with the release of mitochondrial proteins, including cytochrome c and Smac/DIABLO; (iv) accompanied by dissipation of the mitochondrial membrane potential, (v) partially blocked by the inhibition of p38(MAPK) and (vi) mostly abrogated by catalase. In conclusion, the synthetic chalcone is cytotoxic against several types of human leukaemia cell with apoptosis being induced by activation of the extrinsic pathway and the generation of reactive oxygen species.
dc.publisher0009-2797
dc.relation.ispartofChemico-biological interactions (Print)
dc.sourceChemico-Biological Interactions[ISSN 0009-2797],v. 298, p. 137-145
dc.subject.otherActivated Protein-Kinase
dc.subject.otherMitochondrial Dysfunction
dc.subject.otherCancer
dc.subject.otherChalcones
dc.subject.otherInduction
dc.subject.otherProliferation
dc.subject.otherProgression
dc.subject.otherInhibition
dc.subject.otherFlavonols
dc.subject.otherSurvival
dc.title6′-Benzyloxy-4-bromo-2′-hydroxychalcone is cytotoxic against human leukaemia cells and induces caspase-8- and reactive oxygen species-dependent apoptosis
dc.typeinfo:eu-repo/semantics/Article
dc.typeArticle
dc.identifier.doi10.1016/j.cbi.2018.12.010
dc.identifier.scopus85058778065
dc.identifier.isi000454538500018
dc.contributor.authorscopusid57190224633
dc.contributor.authorscopusid57190227049
dc.contributor.authorscopusid6603470039
dc.contributor.authorscopusid8681043500
dc.contributor.authorscopusid7003810011
dc.description.lastpage145
dc.description.firstpage137
dc.relation.volume298
dc.type2Artículo
dc.contributor.daisngid10748553
dc.contributor.daisngid13640797
dc.contributor.daisngid657275
dc.contributor.daisngid128315
dc.contributor.daisngid384944
dc.contributor.wosstandardWOS:Saavedra, E
dc.contributor.wosstandardWOS:Del Rosario, H
dc.contributor.wosstandardWOS:Brouard, I
dc.contributor.wosstandardWOS:Quintana, J
dc.contributor.wosstandardWOS:Estevez, F
dc.date.coverdateEnero 2019
dc.identifier.ulpgces
dc.description.sjr0,896
dc.description.jcr3,723
dc.description.sjrqQ1
dc.description.jcrqQ1
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptBioquímica Farmacológica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptBioquímica Farmacológica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.orcid0000-0001-8225-4538-
crisitem.author.orcid0000-0002-9728-2774-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameQuintana Aguiar, José Martín-
crisitem.author.fullNameEstévez Rosas, Francisco Jesús-
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