Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/54976
DC FieldValueLanguage
dc.contributor.authorRamírez, Gustavo A.en_US
dc.contributor.authorEspinosa de los Monteros, A.en_US
dc.date.accessioned2019-02-18T15:58:28Z-
dc.date.available2019-02-18T15:58:28Z-
dc.date.issued2019en_US
dc.identifier.issn1932-8486en_US
dc.identifier.otherWoS-
dc.identifier.urihttp://hdl.handle.net/10553/54976-
dc.description.abstractMerkel cells (MCs) are neuroendocrine cells involved with tactile sense, growth, differentiation, and homeostasis of the skin as well as in different cutaneous diseases. Specific staining techniques are required for their identification because they are not easily visible in paraffin sections stained with hematoxylin and eosin. The present study assess the histochemical features of the MCs in dogs comparing with those described for other mammals in the literature and with the use of immunohistochemistry. A systematic study of samples from MCs‐rich areas from healthy dogs was carried out by use of several histologic stains, including metachromatic staining, silver stains, methylene blue, periodic acid–Schiff stain, and osmium‐based staining method. MCs were detected by the Grimelius argyrophilic stain in 86.7% of the specimens. The staining was showed as dark‐brown granular cytoplasmic and consistently polarized to the basal cell cytoplasm matching with the cellular distribution of the characteristic neurosecretory granules. Some modifications in the standard staining protocol, including rinsing, silver reimpregnation, and counterstain dye, enhanced the MCs identification in stratified squamous epithelium. When compared with Cytokeratin 20‐immunolabeled serial sections several MCs appeared nonstained with the argyrophilic method. These differences in MC numbers between stains were statistically significant. Other histologic stains failed to identify MCs in the specimens. The results of this study indicate that Grimelius argyrophilic stain is a suitable method for demonstration of MCs in the stratified squamous epithelium of skin and mucosa. Discussion on its utility when compared with immunohistochemistry and a review of the scientific literature is also presented.en_US
dc.languageengen_US
dc.relation.ispartofAnatomical Recorden_US
dc.sourceAnatomical Record [ISSN 1932-8486], v. 302 (8), p. 1458-1464, (Agosto 2019)en_US
dc.subject3109 Ciencias veterinariasen_US
dc.subject.otherArgyrophiliaen_US
dc.subject.otherCK20en_US
dc.subject.otherGrimeliusen_US
dc.subject.otherHistochemistryen_US
dc.subject.otherMerkel cellsen_US
dc.titleStudy on the role of histochemical stains in identifying Merkel Cells in dogsen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1002/ar.24013en_US
dc.identifier.scopus85057443623-
dc.identifier.isi000475664800020-
dc.contributor.authorscopusid25623593400-
dc.contributor.authorscopusid8846556600-
dc.identifier.eissn1932-8494-
dc.description.lastpage1464en_US
dc.identifier.issue8-
dc.description.firstpage1458en_US
dc.relation.volume302en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.contributor.daisngid389144-
dc.contributor.daisngid700159-
dc.description.numberofpages7en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Ramirez, GA-
dc.contributor.wosstandardWOS:de Los Monteros, AE-
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.sjr0,538
dc.description.jcr1,634
dc.description.sjrqQ2
dc.description.jcrqQ3
dc.description.scieSCIE
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR IUSA-ONEHEALTH 3: Histología y Patología Veterinaria y Forense (Terrestre y Marina)-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Morfología-
crisitem.author.orcid0000-0002-7736-3139-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameEspinosa De Los Monteros Y Zayas, Antonio-
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