Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/53730
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dc.contributor.authorMonnerat, MP
dc.contributor.authorThiaucourt, F
dc.contributor.authorPoveda, JB
dc.contributor.authorNicolet, J
dc.contributor.authorFrey, J
dc.contributor.otherPoveda, Jose
dc.date.accessioned2019-02-04T18:00:11Z-
dc.date.available2019-02-04T18:00:11Z-
dc.date.issued1999
dc.identifier.issn1071-412X
dc.identifier.urihttp://hdl.handle.net/10553/53730-
dc.description.abstractThe genes encoding the 62-kDa lipoproteins from the Mycoplasma mycoides subsp, mycoides large-colony type (LC) strain Y-goat and the ill mycoides subsp, capri strain PG3 were cloned and analyzed by sequencing. These two lipoproteins have been named LppA[MmymyLC] and LppA[Mmyca], and their corresponding genes have been named lppA[MmymyLC] and lppA[Mmyca], respectively. The nucleotide and deduced amino acid sequences of these two lipoproteins showed a very high degree of similarity between these two mycoplasmas. Given the sequence data, LppA seems to fulfill the same structural functions as the previously described major lipoproteins P72 of M. mycoides subsp, mycoides small-colony type and P67 of the Mycoplasma species bovine group 7. Based on lppA gene sequences of M. mycoides subsp. mycoides LC and M. mycoides subsp. capri type strains, a specific PCR assay was developed so that it amplified this gene in all field strains of the two species analyzed in this study but not in the other members of the M. mycoides cluster. Analysis of the PCR-amplified lppA genes with frequently cutting restriction enzymes showed a certain degree of genetic variability which, however, did not cluster the two subspecies. This PCR therefore allows a rapid identification of M. mycoides subsp, mycoides LC and M. mycoides subsp. capri but does not distinguish between these two closely related subspecies. LppA was expressed in Escherichia coli K-12 and used for the production of polyclonal mouse antiserum. Antibodies against recombinant LppA[MmymyLC] reacted with a 62-kDa protein in all di. mycoides subsp, mycoides LC and;ll. mycoides subsp, capri type strains and field strains tested but not with the other members of the ill. mycoides cluster, thus showing the antigenic specificity of LppA and further supporting the concept that a close relationship exists between these two mycoplasmas.
dc.publisher1071-412X
dc.relation.ispartofClinical and Diagnostic Laboratory Immunology
dc.sourceClinical And Diagnostic Laboratory Immunology[ISSN 1071-412X],v. 6 (2), p. 224-230
dc.subject.otherPage Protein-Patterns
dc.subject.otherNumerical-Analysis
dc.subject.otherSequence-Analysis
dc.subject.otherEscherichia-Coli
dc.subject.otherColony Type
dc.subject.otherGoats
dc.subject.otherCluster
dc.subject.otherDna
dc.subject.otherSheep
dc.subject.otherPleuropneumonia
dc.titleGenetic and serological analysis of lipoprotein LppA in Mycopasma mycoides subsp mycoides LC and Mycoplasma mycoides subsp capri
dc.typeinfo:eu-repo/semantics/Article
dc.typeArticle
dc.identifier.isi000079046800014
dcterms.isPartOfClinical And Diagnostic Laboratory Immunology
dcterms.sourceClinical And Diagnostic Laboratory Immunology[ISSN 1071-412X],v. 6 (2), p. 224-230
dc.description.lastpage230
dc.identifier.issue2
dc.description.firstpage224
dc.relation.volume6
dc.type2Artículo
dc.identifier.wosWOS:000079046800014
dc.contributor.daisngid10001807
dc.contributor.daisngid583643
dc.contributor.daisngid498394
dc.contributor.daisngid154853
dc.contributor.daisngid28266943
dc.contributor.daisngid67426
dc.identifier.investigatorRIDL-5987-2014
dc.contributor.wosstandardWOS:Monnerat, MP
dc.contributor.wosstandardWOS:Thiaucourt, F
dc.contributor.wosstandardWOS:Poveda, JB
dc.contributor.wosstandardWOS:Nicolet, J
dc.contributor.wosstandardWOS:Frey, J
dc.date.coverdateMarzo 1999
dc.identifier.ulpgces
dc.description.jcr1,583
dc.description.jcrqQ2
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IUSA-ONEHEALTH1: Epidemiología, Medicina Preventiva Veterinaria y Zoonosis-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0002-9846-2427-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNamePoveda Guerrero, José Bismarck-
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