Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/52552
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dc.contributor.authorCugno, G.en_US
dc.contributor.authorParreira, J.R.en_US
dc.contributor.authorFerlizza, E.en_US
dc.contributor.authorHernández Castellano, Lorenzo Enriqueen_US
dc.contributor.authorCarneiro, M.en_US
dc.contributor.authorRenaut, J.en_US
dc.contributor.authorCastro, Noemien_US
dc.contributor.authorArguello, Anastasioen_US
dc.contributor.authorCapote, J.en_US
dc.contributor.authorCampos, A.M.O.en_US
dc.contributor.authorAlmeida, A.M.en_US
dc.contributor.otherScientific output, CIIMAR-
dc.contributor.otherCIISA, FMV-
dc.contributor.otherrenaut, jenny-
dc.contributor.otherHernandez-Castellano, Lorenzo Enrique-
dc.contributor.otherArguello, Anastasio-
dc.contributor.otherCastro, Noemi-
dc.contributor.otherAlmeida, Andre-
dc.contributor.otherScientific Productivity, CIIMAR-
dc.contributor.otherParreira, Jose-
dc.contributor.otherCampos, Alexandre-
dc.contributor.otherFerlizza, Enea-
dc.contributor.otherCarneiro, Mariana-
dc.date.accessioned2018-11-30T09:36:40Z-
dc.date.available2018-11-30T09:36:40Z-
dc.date.issued2016en_US
dc.identifier.issn1932-6203en_US
dc.identifier.otherScopus-
dc.identifier.urihttp://hdl.handle.net/10553/52552-
dc.description.abstractSeasonal weight loss (SWL) is the most important limitation to animal production in the Tropical and Mediterranean regions, conditioning producer's incomes and the nutritional status of rural communities. It is of importance to produce strategies to oppose adverse effects of SWL. Breeds that have evolved in harsh climates have acquired tolerance to SWL through selection. Most of the factors determining such ability are related to changes in biochemical pathways as affected by SWL. In this study, a gel based proteomics strategy (BN: Blue-Native Page and 2DE: Two-dimensional gel electrophoresis) was used to characterize the mitochondrial proteome of the secretory tissue of the goat mammary gland. In addition, we have conducted an investigation of the effects of weight loss in two goat breeds with different levels of adaptation to nutritional stress: Majorera (tolerant) and Palmera (susceptible). The study used Majorera and Palmera dairy goats, divided in 4 sets, 2 for each breed: underfed group fed on wheat straw (restricted diet, so their body weight would be 15-20% reduced by the end of experiment), and a control group fed with an energy-balanced diet. At the end of the experimental period (22 days), mammary gland biopsies were obtained for all experimental groups. The proteomic analysis of the mitochondria enabled the resolution of a total of 277 proteins, and 148 (53%) were identified by MALDI-TOF/TOF mass spectrometry. Some of the proteins were identified as subunits of the glutamate dehydrogenase complex and the respiratory complexes I, II, IV, V from mitochondria, as well as numerous other proteins with functions in: metabolism, development, localization, cellular organization and biogenesis, biological regulation, response to stimulus, among others, that were mapped in both BN and 2DE gels. The comparative proteomics analysis enabled the identification of several proteins: NADH-ubiquinone oxidoreductase 75 kDa subunit and lamin B1 mitochondrial (up-regulated in the Palmera breed), Guanine nucleotide-binding protein G(I)/G(S)/G (T) subunit beta-2 (up-regulated in the Majorera breed) and cytochrome b-c1 complex subunit 1, mitochondrial and Chain D, Bovine F1-C8 Sub-Complex Of Atp Synthase (downregulated in the Majorera breed) as a consequence of weight loss.en_US
dc.languageengen_US
dc.publisher1932-6203-
dc.relation.ispartofPLoS ONEen_US
dc.sourcePlos One[ISSN 1932-6203],v. 11 (3)en_US
dc.subject3109 Ciencias veterinariasen_US
dc.subject.otherAustralian Merino Lambsen_US
dc.subject.otherGenetic-Resourcesen_US
dc.subject.otherAnimal Productionen_US
dc.subject.otherMilk-Productionen_US
dc.subject.otherGuinea-Bissauen_US
dc.subject.otherLive Weighten_US
dc.subject.otherProteinsen_US
dc.subject.otherComplexen_US
dc.subject.otherMuscleen_US
dc.subject.otherIdentificationen_US
dc.titleThe goat (Capra hircus) mammary gland mitochondrial proteome: A study on the effect of weight loss using blue-native page and two-dimensional gel electrophoresisen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0151599en_US
dc.identifier.scopus84975157082-
dc.identifier.isi000373121800015-
dcterms.isPartOfPlos One-
dcterms.sourcePlos One[ISSN 1932-6203],v. 11 (3)-
dc.contributor.authorscopusid56711234400-
dc.contributor.authorscopusid57210330650-
dc.contributor.authorscopusid55953519600-
dc.contributor.authorscopusid36682092900-
dc.contributor.authorscopusid56596277800-
dc.contributor.authorscopusid8731903100-
dc.contributor.authorscopusid57200208399-
dc.contributor.authorscopusid6701710018-
dc.contributor.authorscopusid6602424338-
dc.contributor.authorscopusid56063261600-
dc.contributor.authorscopusid55945318600-
dc.identifier.eissn1932-6203-
dc.identifier.issue3-
dc.relation.volume11en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.wosWOS:000373121800015-
dc.contributor.daisngid8860435-
dc.contributor.daisngid30656913-
dc.contributor.daisngid3139326-
dc.contributor.daisngid34693778-
dc.contributor.daisngid3586545-
dc.contributor.daisngid1033930-
dc.contributor.daisngid4132381-
dc.contributor.daisngid155838-
dc.contributor.daisngid330531-
dc.contributor.daisngid298051-
dc.contributor.daisngid383596-
dc.contributor.daisngid801899-
dc.contributor.daisngid493849-
dc.identifier.investigatorRIDE-5122-2012-
dc.identifier.investigatorRIDD-2242-2016-
dc.identifier.investigatorRIDK-3216-2012-
dc.identifier.investigatorRIDT-1034-2016-
dc.identifier.investigatorRIDB-4493-2010-
dc.identifier.investigatorRIDF-9621-2016-
dc.identifier.investigatorRIDI-4369-2013-
dc.identifier.investigatorRIDN-6498-2017-
dc.identifier.investigatorRIDNo ID-
dc.identifier.investigatorRIDNo ID-
dc.identifier.investigatorRIDNo ID-
dc.identifier.investigatorRIDNo ID-
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Cugno, G-
dc.contributor.wosstandardWOS:Parreira, JR-
dc.contributor.wosstandardWOS:Ferlizza, E-
dc.contributor.wosstandardWOS:Hernandez-Castellano, LE-
dc.contributor.wosstandardWOS:Carneiro, M-
dc.contributor.wosstandardWOS:Renaut, J-
dc.contributor.wosstandardWOS:Castro, N-
dc.contributor.wosstandardWOS:Arguello, A-
dc.contributor.wosstandardWOS:Capote, J-
dc.contributor.wosstandardWOS:Campos, AMO-
dc.contributor.wosstandardWOS:Almeida, AM-
dc.date.coverdateMarzo 2016en_US
dc.identifier.ulpgces
dc.description.sjr1,201
dc.description.jcr2,806
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
dc.description.erihplusERIH PLUS
item.fulltextCon texto completo-
item.grantfulltextopen-
crisitem.author.deptGIR IUSA-ONEHEALTH 4. Producción y Biotecnología Animal-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR IUSA-ONEHEALTH 4. Producción y Biotecnología Animal-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR IUSA-ONEHEALTH 4. Producción y Biotecnología Animal-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0003-2729-0434-
crisitem.author.orcid0000-0002-3026-2031-
crisitem.author.orcid0000-0002-4426-0678-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameHernández Castellano, Lorenzo Enrique-
crisitem.author.fullNameCastro Navarro, Noemí-
crisitem.author.fullNameArgüello Henríquez, Anastasio-
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