Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/52431
DC FieldValueLanguage
dc.contributor.authorDescalzo, Silvia Muñozen_US
dc.contributor.authorRué, Pauen_US
dc.contributor.authorGarcia-Ojalvo, Jordien_US
dc.contributor.authorArias, Alfonso Martinezen_US
dc.date.accessioned2018-11-25T20:16:39Z-
dc.date.available2018-11-25T20:16:39Z-
dc.date.issued2012en_US
dc.identifier.issn1066-5099en_US
dc.identifier.urihttp://hdl.handle.net/10553/52431-
dc.description.abstractThe pluripotent state is traditionally associated with large absolute levels of certain transcription factors such as Nanog and Oct4. Here, we present experimental observations using quantitative immunofluorescence that pluripotency in mouse embryonic stem cells (mESCs) is established by specific ratios between Oct4 and Nanog. When cells are grown in 2i conditions, they exhibit uniform levels of pluripotency and this is associated with a high correlation between the levels of Oct4 and Nanog in individual cells. The correlation is lost when cells differentiate. Our results suggest that the correlation between these two factors and the distribution of Oct4/Nanog ratios can be used as quantifiers to distinguish between three subpopulations in an mESC culture: pluripotent, lineage-primed, and differentiating cells. When we apply these quantifiers to cells with lower levels of Nanog or mutant for β-Catenin or Tcf3, the results suggest that these cells exhibit higher probability of differentiation.en_US
dc.languageengen_US
dc.relation.ispartofStem Cellsen_US
dc.sourceStem Cells[ISSN 1066-5099],v. 30(12), p. 2683-2691 8Diciembre 2012)en_US
dc.subject32 Ciencias médicasen_US
dc.subject2407 Biología celularen_US
dc.subject.otherEmbryonic stem cellsen_US
dc.subject.otherPluripotent stem cellsen_US
dc.subject.otherSelf-renewalen_US
dc.subject.otherSerum-freeen_US
dc.subject.otherTranscription factorsen_US
dc.titleCorrelations between the levels of Oct4 and Nanog as a signature for naïve pluripotency in mouse embryonic stem cellsen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1002/stem.1230en_US
dc.identifier.scopus84870312110-
dc.contributor.authorscopusid9235908900-
dc.contributor.authorscopusid35189636800-
dc.contributor.authorscopusid55931225400-
dc.contributor.authorscopusid57204246803-
dc.description.lastpage2691en_US
dc.identifier.issue12-
dc.description.firstpage2683en_US
dc.relation.volume30en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.description.numberofpages9en_US
dc.utils.revisionen_US
dc.date.coverdateDiciembre 2012en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.sjr4,036-
dc.description.jcr7,701-
dc.description.sjrqQ1-
dc.description.jcrqQ1-
dc.description.scieSCIE-
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR IUIBS: Diabetes y endocrinología aplicada-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.orcid0000-0003-0939-7721-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameMuñoz Descalzo, Silvia-
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