Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/52429
DC FieldValueLanguage
dc.contributor.authorArias, Alfonso Martinezen_US
dc.contributor.authorFaunes, Fernandoen_US
dc.contributor.authorHayward, Penelopeen_US
dc.contributor.authorDescalzo, Silvia Muñozen_US
dc.contributor.authorChatterjee, Sujash S.en_US
dc.contributor.authorBalayo, Tinaen_US
dc.contributor.authorTrott, Jamieen_US
dc.contributor.authorChristoforou, Andrewen_US
dc.contributor.authorFerrer-Vaquer, Annaen_US
dc.contributor.authorHadjantonakis, Anna Katerinaen_US
dc.contributor.authorDasgupta, Ramanujen_US
dc.date.accessioned2018-11-25T20:15:39Z-
dc.date.available2018-11-25T20:15:39Z-
dc.date.issued2013en_US
dc.identifier.issn0950-1991en_US
dc.identifier.urihttp://hdl.handle.net/10553/52429-
dc.description.abstractThe maintenance of pluripotency in mouse embryonic stem cells (mESCs) relies on the activity of a transcriptional network that is fuelled by the activity of three transcription factors (Nanog, Oct4 and Sox2) and balanced by the repressive activity of Tcf3. Extracellular signals modulate the activity of the network and regulate the differentiation capacity of the cells. Wnt/β-catenin signaling has emerged as a significant potentiator of pluripotency: increases in the levels of β-catenin regulate the activity of Oct4 and Nanog, and enhance pluripotency. A recent report shows that β-catenin achieves some of these effects by modulating the activity of Tcf3, and that this effect does not require its transcriptional activation domain. Here, we show that during self-renewal there is negligible transcriptional activity of β-catenin and that this is due to its tight association with membranes, where we find it in a complex with Oct4 and E-cadherin. Differentiation triggers a burst of Wnt/β-catenin transcriptional activity that coincides with the disassembly of the complex. Our results establish that β-catenin, but not its transcriptional activity, is central to pluripotency acting through a β-catenin/Oct4 complex.en_US
dc.languageengen_US
dc.relation.ispartofDevelopment (Cambridge)en_US
dc.sourceDevelopment (Cambridge)[ISSN 0950-1991],v. 140(6), p. 1171-1183en_US
dc.subject32 Ciencias médicasen_US
dc.subject240107 Embriología animalen_US
dc.subject.otherOct4en_US
dc.subject.otherWnt signalingen_US
dc.subject.otherMouse embryonic stem cellsen_US
dc.subject.otherPluripotencyen_US
dc.subject.otherβ-Cateninen_US
dc.titleA membrane-associated β-catenin/Oct4 complex correlates with ground-state pluripotency in mouse embryonic stem cellsen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1242/dev.085654en_US
dc.identifier.scopus84874512754-
dc.contributor.authorscopusid57204246803-
dc.contributor.authorscopusid6505818230-
dc.contributor.authorscopusid10142112100-
dc.contributor.authorscopusid9235908900-
dc.contributor.authorscopusid50061046700-
dc.contributor.authorscopusid8406257400-
dc.contributor.authorscopusid55007157400-
dc.contributor.authorscopusid35067811000-
dc.contributor.authorscopusid18434264400-
dc.contributor.authorscopusid6701560632-
dc.contributor.authorscopusid7005395349-
dc.description.lastpage1183en_US
dc.identifier.issue6-
dc.description.firstpage1171en_US
dc.relation.volume140en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.description.numberofpages13en_US
dc.utils.revisionen_US
dc.date.coverdateMarzo 2013en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.sjr5,693-
dc.description.jcr6,273-
dc.description.sjrqQ1-
dc.description.jcrqQ1-
dc.description.scieSCIE-
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IUIBS: Diabetes y endocrinología aplicada-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.orcid0000-0003-0939-7721-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameMuñoz Descalzo,Silvia-
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