Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/52065
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dc.contributor.authorRamirez, A. S.
dc.contributor.authorNaylor, C. J.
dc.contributor.authorPitcher, D. G.
dc.contributor.authorBradbury, J. M.
dc.contributor.otherRamirez, Ana
dc.contributor.otherRamirez, Ana S.
dc.date.accessioned2018-11-25T17:09:39Z-
dc.date.available2018-11-25T17:09:39Z-
dc.date.issued2008
dc.identifier.issn0378-1135
dc.identifier.urihttp://hdl.handle.net/10553/52065-
dc.description.abstractIn order to investigate its value for phylogenetic analysis, species characterisation and diagnosis, the 16S-23S rDNA intergenic spacer regions (ISRs) of the type strain of 23 avian Mycoplasma species were amplified and the sequences determined. Also sequenced were the reference strains of Mycoplasma iowae serotypes J, K, N, Q and R and a number of field strains of Mycoplasma synoviae, Mycoplasma gallisepticum, Mycoplasma meleagridis and M. iowae. The ISRs demonstrated a high level of size variation (178-2488 bp) between species and did not include RNA genes. Phylogenetic analysis performed using the information conflicted with that based on the 16S rDNA and was therefore not helpful for phylogenetic studies. However, the ISR did appear to be of value for determining species since there was high inter-species variation between all 23 avian Mycoplasma species, and in addition there was low intra-species variation, at least in the four pathogenic species. It could also be very useful as additional information in the description of a new species and as a target for species-specific PCRs. (C) 2007 Elsevier B.V. All rights reserved.
dc.publisher0378-1135
dc.relation.ispartofVeterinary Microbiology
dc.sourceVeterinary Microbiology[ISSN 0378-1135],v. 128 (3-4), p. 279-287
dc.subject.otherRibosomal-Rna Spacer
dc.subject.otherPolymerase-Chain-Reaction
dc.subject.otherEt-Al. 1989
dc.subject.otherRegion
dc.subject.other16S
dc.subject.otherPrimers
dc.subject.otherCluster
dc.subject.otherGene
dc.subject.otherPcr
dc.subject.otherMollicutes
dc.titleHigh inter-species and low intra-species variation in 16S-23S rDNA spacer sequences of pathogenic avian mycoplasmas offers potential use as a diagnostic tool
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1016/j.vetmic.2007.10.023
dc.identifier.scopus39749182326
dc.identifier.isi000254721200007
dcterms.isPartOfVeterinary Microbiology
dcterms.sourceVeterinary Microbiology[ISSN 0378-1135],v. 128 (3-4), p. 279-287
dc.contributor.authorscopusid7401735183
dc.contributor.authorscopusid7102949185
dc.contributor.authorscopusid56250688300
dc.contributor.authorscopusid7201391094
dc.description.lastpage287
dc.description.firstpage279
dc.relation.volume128
dc.type2Artículoes
dc.identifier.wosWOS:000254721200007
dc.contributor.daisngid1521915
dc.contributor.daisngid1049516
dc.contributor.daisngid1961058
dc.contributor.daisngid351260
dc.identifier.investigatorRIDL-5255-2014
dc.identifier.investigatorRIDNo ID
dc.contributor.wosstandardWOS:Ramirez, AS
dc.contributor.wosstandardWOS:Naylor, CJ
dc.contributor.wosstandardWOS:Pitcher, DG
dc.contributor.wosstandardWOS:Bradbury, JM
dc.date.coverdateAbril 2008
dc.identifier.ulpgces
dc.description.jcr2,37
dc.description.jcrqQ1
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IUSA-ONEHEALTH1: Epidemiología, Medicina Preventiva Veterinaria y Zoonosis-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0002-8721-775X-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameRamírez Corbera, Ana Sofía-
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