Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/52063
Campo DC Valoridioma
dc.contributor.authorRamírez, Ana S.
dc.contributor.authorNaylor, Clive J.
dc.contributor.authorYavari, Christine A.
dc.contributor.authorDare, Cynthia M.
dc.contributor.authorBradbury, Janet M.
dc.date.accessioned2018-11-25T17:08:39Z-
dc.date.available2018-11-25T17:08:39Z-
dc.date.issued2011
dc.identifier.issn0307-9457
dc.identifier.urihttp://hdl.handle.net/10553/52063-
dc.description.abstractMycoplasma synoviae has been associated with economic loss in the chicken and turkey industries. The molecular characterization of M. synoviae at strain level allows the analysis of relationships between strains that may be valuable in epidemiological investigations. In the present study, the intergenic spacer region (ISR) between the 16S and 23S rRNA genes was examined to see whether useful information about strains could be derived. M. synoviae has two copies of this region, which may not be exactly the same (intercistronic heterogeneity). Sequencing of the ISRs of 21 M. synoviae isolates and the type strain revealed that 19 of them had such heterogeneity so DNA cloning was performed where necessary. All sequences were analysed and aligned; the percentage similarity of the DNA was calculated and a dendrogram was constructed. The length of the ISRs varied between 305 and 309 base pairs. Apart from having extra A/Ts in poly-A or poly-T regions and the presence of a few polymorphisms, the sequences of the M. synoviae strains were similar. Based on phylogenetic analysis, the strains were assigned to 10 groupstaking into account that within each group the DNA similarity was 100%, while the lowest similarity between groups was 95.8%. The results were compared with those obtained with the vlhA gene, resulting in very similar M. synoviae groups. Although the ISR could be a good target for strain typing, as has been shown by others for Mycoplasma gallisepticum, the method may be too cumbersome for routine use with M. synoviae because of complications with intercistronic heterogeneity. However, if the ISR sequence information was to be combined with other mutation detection techniques it could increase the discriminatory power.
dc.publisher0307-9457
dc.relation.ispartofAvian Pathology
dc.sourceAvian Pathology[ISSN 0307-9457],v. 40, p. 79-86
dc.subject.otherFragment-Length-Polymorphism
dc.subject.otherPolymerase-Chain-Reaction
dc.subject.otherRestriction Endonuclease Analysis
dc.subject.otherEncoding Gene Vlha
dc.subject.otherIntraspecies Heterogeneity
dc.subject.otherGel-Electrophoresis
dc.subject.otherDiagnostic Pcr
dc.subject.otherSequence
dc.subject.otherIdentification
dc.subject.otherGallisepticum
dc.titleAnalysis of the 16S to 23S rRNA intergenic spacer region of Mycoplasma synoviae field strains
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1080/03079457.2010.537305
dc.identifier.scopus79951704628
dc.identifier.isi000287413700009
dc.contributor.authorscopusid7401735183
dc.contributor.authorscopusid7102949185
dc.contributor.authorscopusid6507952277
dc.contributor.authorscopusid36840692500
dc.contributor.authorscopusid55921969600
dc.description.lastpage86
dc.description.firstpage79
dc.relation.volume40
dc.type2Artículoes
dc.contributor.daisngid1521915
dc.contributor.daisngid1049516
dc.contributor.daisngid3393127
dc.contributor.daisngid596455
dc.contributor.daisngid351260
dc.contributor.wosstandardWOS:Ramirez, AS
dc.contributor.wosstandardWOS:Naylor, CJ
dc.contributor.wosstandardWOS:Yavari, CA
dc.contributor.wosstandardWOS:Dare, CM
dc.contributor.wosstandardWOS:Bradbury, JM
dc.date.coverdateFebrero 2011
dc.identifier.ulpgces
dc.description.sjr0,83
dc.description.jcr1,711
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR IUSA-ONEHEALTH1: Epidemiología, Medicina Preventiva Veterinaria y Zoonosis-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0002-8721-775X-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameRamírez Corbera, Ana Sofía-
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