Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/51921
DC FieldValueLanguage
dc.contributor.authorVincke, Cécile
dc.contributor.authorGutiérrez, Carlos
dc.contributor.authorWernery, Ulrich
dc.contributor.authorDevoogdt, Nick
dc.contributor.authorHassanzadeh-Ghassabeh, Gholamreza
dc.contributor.authorMuyldermans, Serge
dc.date.accessioned2018-11-25T05:26:17Z-
dc.date.available2018-11-25T05:26:17Z-
dc.date.issued2012
dc.identifier.isbn9781617799730
dc.identifier.issn1064-3745
dc.identifier.urihttp://hdl.handle.net/10553/51921-
dc.description.abstractImmunizing a camelid (camels and llamas) with soluble, properly folded proteins raises an affinity-matured immune response in the unique camelid heavy-chain only antibodies (HCAbs). The peripheral blood lymphocytes of the immunized animal are used to clone the antigen-binding antibody fragment from the HCAbs in a phage display vector. A representative aliquot of the library of these antigen-binding fragments is used to retrieve single domain antigen-specific binders by successive rounds of panning. These single domain antibody fragments are cloned in tandem to generate manifold constructs (bivalent, biparatopic or bispecific constructs) to increase their functional affinity, to increase specificity, or to connect two independent antigen molecules. © 2012 Springer Science+Business Media, LLC.
dc.publisher1064-3745
dc.relation.ispartofMethods in molecular biology (Clifton, N.J.)
dc.sourceMethods in Molecular Biology[ISSN 1064-3745],v. 907, p. 145-176
dc.titleGeneration of single domain antibody fragments derived from camelids and generation of manifold constructs
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1007/978-1-61779-974-7-8
dc.identifier.scopus84867755216
dc.contributor.authorscopusid8622097100
dc.contributor.authorscopusid7202545218
dc.contributor.authorscopusid7005483197
dc.contributor.authorscopusid9268219700
dc.contributor.authorscopusid25645875200
dc.contributor.authorscopusid7005810441
dc.description.lastpage176
dc.description.firstpage145
dc.relation.volume907
dc.type2Artículoes
dc.date.coverdateOctubre 2012
dc.identifier.ulpgces
dc.description.sjr0,699
dc.description.sjrqQ4
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.orcid0000-0003-0764-7408-
crisitem.author.fullNameGutiérrez Cabrera, Carlos Javier-
Appears in Collections:Artículos
Show simple item record

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.