Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/51554
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dc.contributor.authorBatista, M.
dc.contributor.authorAlamo, D.
dc.contributor.authorGonzález, F.
dc.contributor.authorCruz, M. G.
dc.contributor.authorGracia, A.
dc.date.accessioned2018-11-25T01:38:59Z-
dc.date.available2018-11-25T01:38:59Z-
dc.date.issued2006
dc.identifier.issn0936-6768
dc.identifier.urihttp://hdl.handle.net/10553/51554-
dc.description.abstractThis study was carried out to assess the in vitro quality of canine semen frozen in an ultrafreezer at -152 degrees C and to evaluate the male-to-male variation of frozen semen in five male dogs of the Canarian Mastiff breed. Four ejaculates of each dog were processed individually (5% glycerol and 0.5% Equex) to reach a final concentration of 100 x 10(6) spermatozoa/ml. Then, two freezing techniques were tested to assess the seminal quality (sperm motility, live spermatozoa and abnormal sperm cell percentages) at 1, 30, 60, 120 and 360 days after freezing: (i) semen was frozen and stored in liquid nitrogen; (ii) semen was frozen and stored in the ultrafreezer at -152 degrees C. After freezing-thawing, both freezing protocols showed no significant differences in sperm motility and the percentages of live and abnormal spermatozoa. On the other hand, the microscopic characteristics of spermatozoa in fresh semen were practically similar among males; however, after the semen processing and freezing, significant differences were observed (p < 0.05) among males, especially as regards sperm motility. This inter-individual variability was detected in both freezing protocols, showing that the male-to-male variation in the seminal quality post-freezing was independent of the freezing technique used. The in vitro results obtained in the Canarian Mastiff breed confirmed that the use of ultra-freezers at -152 degrees C is a potential alternative to liquid nitrogen for storing canine semen for long periods of time.
dc.publisher0936-6768
dc.relation.ispartofReproduction in Domestic Animals
dc.sourceReproduction in Domestic Animals[ISSN 0936-6768],v. 41, p. 423-428
dc.subject.otherCanine Spermatozoa
dc.subject.otherIntrauterine Insemination
dc.subject.otherArtificial-Insemination
dc.subject.otherFertility Parameters
dc.subject.otherMotility Patterns
dc.subject.otherPostthaw Survival
dc.subject.other3 Extenders
dc.subject.otherCryopreservation
dc.subject.otherFrozen
dc.subject.otherFresh
dc.titleInfluence of the freezing technique (nitrogen liquid vs ultrafreezer of -152°C) and male-to-male variation over the semen quality in Canarian Mastiff breed dogs
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1111/j.1439-0531.2006.00687.x
dc.identifier.scopus33748761628
dc.identifier.isi000240565700007
dc.contributor.authorscopusid6506315360
dc.contributor.authorscopusid6507009446
dc.contributor.authorscopusid57194243767
dc.contributor.authorscopusid40161215300
dc.contributor.authorscopusid7006185082
dc.description.lastpage428
dc.description.firstpage423
dc.relation.volume41
dc.type2Artículoes
dc.contributor.daisngid30341669
dc.contributor.daisngid1791005
dc.contributor.daisngid3001453
dc.contributor.daisngid28313379
dc.contributor.daisngid9536463
dc.contributor.wosstandardWOS:Batista, M
dc.contributor.wosstandardWOS:Alamo, D
dc.contributor.wosstandardWOS:Gonzalez, F
dc.contributor.wosstandardWOS:Cruz, MG
dc.contributor.wosstandardWOS:Gracia, A
dc.date.coverdateOctubre 2006
dc.identifier.ulpgces
dc.description.jcr1,503
dc.description.jcrqQ1
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IUIBS: Medicina Veterinaria e Investigación Terapéutica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR IUSA-ONEHEALTH 5: Reproducción Animal, Oncología y Anestesiología Comparadas-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0001-9753-4786-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameBatista Arteaga, Miguel-
crisitem.author.fullNameGracia Molina, Anselmo-
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