Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/51343
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dc.contributor.authorPeyraud, A.
dc.contributor.authorWoubit, S.
dc.contributor.authorPoveda, JB
dc.contributor.authorDe La Fe, C.
dc.contributor.authorMercier, P.
dc.contributor.authorThiaucourt, F.
dc.contributor.otherDe la Fe, Christian
dc.contributor.otherPoveda, Jose
dc.date.accessioned2018-11-24T23:45:44Z-
dc.date.available2018-11-24T23:45:44Z-
dc.date.issued2003
dc.identifier.issn0890-8508
dc.identifier.urihttp://hdl.handle.net/10553/51343-
dc.description.abstractMycoplasma putrefaciens is listed as one of the etiologic agents of the contagious agalactia syndrome by the world organisation for animal health. This species has been characterized only recently, 1974, and the number of outbreaks caused by this microorganism so far is very scarce. It induces mastitis in infected goats although other symptoms such as arthritis in adults and septicaemia in kids are also frequently described. Up to now, the identification of M. putrefaciens relied on classical isolation and identification techniques which present a number of limitations. Specific primers for PCR have been designed based on sequence comparisons of the Arc B gene among the 'Mycoplasma mycoides cluster' and related species such as Mycoplasma cottewii and Mycoplasma yeatsii. Sequence alignments confirmed the taxonomic position of M. putrefaciens, which is related to the W. mycoides cluster' but also very close to M. yeatsii. The polymorphism observed amongst the different Arc B sequences allowed the determination of a primer pair yielding a specific amplification of a 316 bp-long DNA fragment by PCR. This PCR was validated in two different laboratories with a variety of mycoplasma strains isolated from goats. This new PCR technique will be very useful for a quicker determination of M. putrefaciens strains as well as a better understanding of the prevalence of M. putrefaciens infections. (C) 2003 Elsevier Ltd. All rights reserved.
dc.publisher0890-8508
dc.relation.ispartofMolecular and Cellular Probes
dc.sourceMolecular And Cellular Probes[ISSN 0890-8508],v. 17 (6), p. 289-294
dc.subject.otherRibosomal-Rna Genes
dc.subject.otherCaprine Mycoplasmosis
dc.subject.otherMycoides Cluster
dc.subject.otherSequence-Analysis
dc.subject.otherPhylogeny
dc.subject.otherCottewii
dc.subject.otherMastitis
dc.subject.otherAuris
dc.titleA specific PCR for the detection of Mycoplasma putrefaciens, one of the agents of the contagious agalactia syndrome of goats
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1016/j.mcp.2003.07.006
dc.identifier.scopus0242288850
dc.identifier.isi000186737600004
dcterms.isPartOfMolecular And Cellular Probes
dcterms.sourceMolecular And Cellular Probes[ISSN 0890-8508],v. 17 (6), p. 289-294
dc.contributor.authorscopusid7007175014
dc.contributor.authorscopusid6507865676
dc.contributor.authorscopusid7005736558
dc.contributor.authorscopusid9036230000
dc.contributor.authorscopusid16043419700
dc.contributor.authorscopusid7004635802
dc.description.lastpage294
dc.description.firstpage289
dc.relation.volume17
dc.type2Artículoes
dc.identifier.wosWOS:000186737600004
dc.contributor.daisngid5906793
dc.contributor.daisngid9991705
dc.contributor.daisngid498394
dc.contributor.daisngid513821
dc.contributor.daisngid1088156
dc.contributor.daisngid583643
dc.identifier.investigatorRIDF-3373-2016
dc.identifier.investigatorRIDL-5987-2014
dc.contributor.wosstandardWOS:Peyraud, A
dc.contributor.wosstandardWOS:Woubit, S
dc.contributor.wosstandardWOS:Poveda, JB
dc.contributor.wosstandardWOS:De la Fe, C
dc.contributor.wosstandardWOS:Mercier, P
dc.contributor.wosstandardWOS:Thiaucourt, F
dc.date.coverdateEnero 2003
dc.identifier.ulpgces
dc.description.jcr1,345
dc.description.jcrqQ2
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IUSA-ONEHEALTH1: Epidemiología, Medicina Preventiva Veterinaria y Zoonosis-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0002-9846-2427-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNamePoveda Guerrero, José Bismarck-
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