Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/51338
Título: The occurrence of mycoplasmas in the lungs of swine in Gran Canaria (Spain)
Autores/as: Assunção, P.
De la Fe, C.
Kokotovic, B.
González, O. 
Poveda, J. B. 
Palabras clave: Polymerase-Chain-Reaction
Ribosomal-Rna Gene
Respiratory-Tract
Hyopneumoniae
Pigs, et al.
Fecha de publicación: 2005
Editor/a: 0165-7380
Publicación seriada: Veterinary Research Communications 
Resumen: The study was conducted to investigate the mycoplasmal flora in the lungs of pigs with enzootic pneumonia at Gran Canaria (Spain). From 54 pneumonic lungs collected at an abattoir, 85 isolates were cultivated. On the basis of cultural and biochemical characteristics, the isolates were preliminarily identified as Mycoplasma species. Using different species-specific PCRs, 40, 27, 11 and 7 of the isolates were identified as M hyorhinis, M. hyopneumoniae, M. hyosynoviae and M. flocculare, respectively. Nine of the M. hyopneumoniae cultures were found to be in mixed culture with M. flocculare as demonstrated by PCR. By use of a M. flocculare antiserum it was possible to eliminate M. flocculare from M. hyopneumoniae mixed cultures. This study is the first report on isolation of porcine mycoplasmas at Gran Canaria (Spain).
URI: http://hdl.handle.net/10553/51338
ISSN: 0165-7380
DOI: 10.1007/s11259-005-1866-3
Fuente: Veterinary Research Communications[ISSN 0165-7380],v. 29, p. 453-462
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