Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/51334
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dc.contributor.authorAssunção, P.
dc.contributor.authorDe La Fe, C.
dc.contributor.authorAntunes, N. T.
dc.contributor.authorRosales, R. S.
dc.contributor.authorRuiz De Galarreta, C. M.
dc.contributor.authorPoveda, JB
dc.contributor.otherRosales, Ruben
dc.contributor.otherDe la Fe, Christian
dc.contributor.otherPoveda, Jose
dc.contributor.otherRosales, Ruben S.
dc.date.accessioned2018-11-24T23:40:56Z-
dc.date.available2018-11-24T23:40:56Z-
dc.date.issued2006
dc.identifier.issn1364-5072
dc.identifier.urihttp://hdl.handle.net/10553/51334-
dc.description.abstractAims: The potential of using flow cytometry (FC) in combination with a fluorescent dye (SYBR green-I) for rapidly estimating Mycoplasma mycoides subSPS. mycoides large-colony type (MmmLC) in broth culture was examined.Methods and Results: The FC analysis was performed by staining the MmmLC cells with a fluorescent dye, SYBR green-I (SYBR), and the results were compared with plate count method (colony forming units, - CFUs). There was a good correlation (linear regression, r(2) = 0.93) between mycoplasma counts determined by FC (cells ml(-1)) and by traditional plate count method (CFU ml(-1)). The lowest bacterial concentration detected by FC and traditional plate count was of the order of 10(4) cells ml(-1) and 10(3) CFU ml(-1), respectively. FC method allowed results in 20-30 min, whereas at least 24 h were necessary to obtain results with the traditional plate count method (CFU).Conclusion: Growth rates of MmmLC in broth medium determined by FC were highly reproducible and correlated well with mycoplasma counts assessed by the plate count method.Significance and Impact of the Study: These findings suggest that FC could be a good alternative to replace other time-consuming techniques that are currently used to enumerate mycoplasma in broth medium, such as plate count method (CFU).
dc.publisher1364-5072
dc.relation.ispartofJournal of Applied Microbiology
dc.sourceJournal Of Applied Microbiology[ISSN 1364-5072],v. 100 (4), p. 878-884
dc.subject.otherNucleic-Acid Content
dc.subject.otherContagious Agalactia
dc.subject.otherGrowth
dc.subject.otherBacteria
dc.subject.otherHyopneumoniae
dc.subject.otherFlocculare
dc.subject.otherCulture
dc.subject.otherViruses
dc.subject.otherSamples
dc.titleUse of flow cytometry for enumeration of Mycoplasma mycoides subsp. mycoides large-colony type in broth medium
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1111/j.1365-2672.2005.02858.x
dc.identifier.scopus33645103962
dc.identifier.isi000236024400030
dcterms.isPartOfJournal Of Applied Microbiology
dcterms.sourceJournal Of Applied Microbiology[ISSN 1364-5072],v. 100 (4), p. 878-884
dc.contributor.authorscopusid6701838348
dc.contributor.authorscopusid9036230000
dc.contributor.authorscopusid57196635499
dc.contributor.authorscopusid8552025600
dc.contributor.authorscopusid7003806034
dc.contributor.authorscopusid7005736558
dc.description.lastpage884
dc.description.firstpage878
dc.relation.volume100
dc.type2Artículoes
dc.identifier.wosWOS:000236024400030
dc.contributor.daisngid21575212
dc.contributor.daisngid513821
dc.contributor.daisngid1398738
dc.contributor.daisngid907575
dc.contributor.daisngid1664323
dc.contributor.daisngid498394
dc.identifier.investigatorRIDA-5679-2013
dc.identifier.investigatorRIDF-3373-2016
dc.identifier.investigatorRIDL-5987-2014
dc.identifier.investigatorRIDNo ID
dc.contributor.wosstandardWOS:Assuncao, P
dc.contributor.wosstandardWOS:de la Fe, C
dc.contributor.wosstandardWOS:Antunes, NT
dc.contributor.wosstandardWOS:Rosales, RS
dc.contributor.wosstandardWOS:de Galarreta, CMR
dc.contributor.wosstandardWOS:Poveda, JB
dc.date.coverdateAbril 2006
dc.identifier.ulpgces
dc.description.jcr2,206
dc.description.jcrqQ2
dc.description.scieSCIE
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR IUSA-ONEHEALTH1: Epidemiología, Medicina Preventiva Veterinaria y Zoonosis-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR IUSA-ONEHEALTH1: Epidemiología, Medicina Preventiva Veterinaria y Zoonosis-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0003-3706-0181-
crisitem.author.orcid0000-0002-9846-2427-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameRosales Santana, Rubén Sebastián-
crisitem.author.fullNamePoveda Guerrero, José Bismarck-
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