Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/51326
Campo DC Valoridioma
dc.contributor.authorAssunção, Patricia
dc.contributor.authorDavey, Hazel M.
dc.contributor.authorRosales, Ruben S.
dc.contributor.authorAntunes, Nuno T.
dc.contributor.authorDe La Fe, Christian
dc.contributor.authorRamirez, Ana S.
dc.contributor.authorRuiz De Galarreta, Carlos M.
dc.contributor.authorPoveda, Jose B.
dc.contributor.otherDavey, Hazel
dc.contributor.otherRosales, Ruben
dc.contributor.otherPoveda, Jose
dc.contributor.otherRamirez, Ana
dc.contributor.otherDe la Fe, Christian
dc.contributor.otherRamirez, Ana S.
dc.contributor.otherRosales, Ruben S.
dc.date.accessioned2018-11-24T23:36:10Z-
dc.date.available2018-11-24T23:36:10Z-
dc.date.issued2007
dc.identifier.issn1552-4922
dc.identifier.urihttp://hdl.handle.net/10553/51326-
dc.description.abstractThe detection of mycoplasma in milk can be performed by either culture techniques or polymerase chain reaction (PCR) based methods. Although PCR can reduce the average diagnostic time to 5 h in comparison with the several days for the isolation of the agent, there is still a need to develop methods, which could give earlier results. For this purpose, we tested the ability of flow cytometry (FC) to detect mycoplasmas in milk samples. Milk samples inoculated with four different mycoplasmas, Mycoplasma agalactiae, Mycoplasma putrefaciens, Mycoplasma capricolum subsp. Capricolum, or Mycoplasma mycoides subsp. mycoides large-colony type, known to cause contagious agalactia in goats, were stained with the DNA stain SYBR Green I and analyzed by FC. Three goat milk samples, from which mycoplasmas have been isolated in broth medium were also analyzed. All mycoplasmas were easily distinguished from debris of milk samples, but it was not possible to distinguish between the different mycoplasma species. In our conditions, the detection limit of the technique was of the order of 10(3)-10(4) Cells ml(-1). Furthermore, mycoplasmas were also distinguished from Staphylococcus aureus. FC together with SYBR Green I was able to distinguish between mycoplasma cells and debris present in milk samples and gave results in 20-30 min. This is an important first step in developing a robust, routine flow cytometric method for the detection of mycoplasmas in milk samples. (c) 2007 International Society for Analytical Cytology.
dc.publisher1552-4922
dc.relation.ispartofCytometry Part A
dc.sourceCytometry Part A[ISSN 1552-4922],v. 71A (12), p. 1034-1038
dc.subject.otherBulk Tank Milk
dc.subject.otherSybr-Green-I
dc.subject.otherContagious Agalactia
dc.subject.otherSmall Ruminants
dc.subject.otherRapid Detection
dc.subject.otherBacteria
dc.subject.otherQuantification
dc.subject.otherInfections
dc.subject.otherDiagnosis
dc.subject.otherProducts
dc.titleDetection of mycoplasmas in goat milk by flow cytometry
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1002/cyto.a.20476
dc.identifier.scopus36849077598
dc.identifier.isi000251475300009
dcterms.isPartOfCytometry Part A
dcterms.sourceCytometry Part A[ISSN 1552-4922],v. 71A (12), p. 1034-1038
dc.contributor.authorscopusid6701838348
dc.contributor.authorscopusid7003990425
dc.contributor.authorscopusid8552025600
dc.contributor.authorscopusid57196635499
dc.contributor.authorscopusid9036230000
dc.contributor.authorscopusid7401735183
dc.contributor.authorscopusid7003806034
dc.contributor.authorscopusid7005736558
dc.description.lastpage1038
dc.description.firstpage1034
dc.relation.volume71A
dc.type2Artículoes
dc.identifier.wosWOS:000251475300009
dc.contributor.daisngid21575212
dc.contributor.daisngid1510729
dc.contributor.daisngid907575
dc.contributor.daisngid1398738
dc.contributor.daisngid513821
dc.contributor.daisngid1521915
dc.contributor.daisngid27006692
dc.contributor.daisngid33274459
dc.contributor.daisngid498394
dc.identifier.investigatorRIDC-9055-2009
dc.identifier.investigatorRIDA-5679-2013
dc.identifier.investigatorRIDL-5987-2014
dc.identifier.investigatorRIDL-5255-2014
dc.identifier.investigatorRIDF-3373-2016
dc.identifier.investigatorRIDNo ID
dc.identifier.investigatorRIDNo ID
dc.contributor.wosstandardWOS:Assuncao, P
dc.contributor.wosstandardWOS:Davey, HM
dc.contributor.wosstandardWOS:Rosales, RS
dc.contributor.wosstandardWOS:Antunes, NT
dc.contributor.wosstandardWOS:de la Fe, C
dc.contributor.wosstandardWOS:Ramirez, AS
dc.contributor.wosstandardWOS:Ruiz de Galarreta, CM
dc.contributor.wosstandardWOS:Poveda, JB
dc.date.coverdateDiciembre 2007
dc.identifier.ulpgces
dc.description.jcr2,978
dc.description.jcrqQ2
dc.description.scieSCIE
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR IUSA-ONEHEALTH1: Epidemiología, Medicina Preventiva Veterinaria y Zoonosis-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR IUSA-ONEHEALTH1: Epidemiología, Medicina Preventiva Veterinaria y Zoonosis-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR IUSA-ONEHEALTH1: Epidemiología, Medicina Preventiva Veterinaria y Zoonosis-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0003-3706-0181-
crisitem.author.orcid0000-0002-8721-775X-
crisitem.author.orcid0000-0002-9846-2427-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameRosales Santana, Rubén Sebastián-
crisitem.author.fullNameRamírez Corbera, Ana Sofía-
crisitem.author.fullNamePoveda Guerrero, José Bismarck-
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