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Title: Cryptosporidium oocysts in water for human consumption - comparison of staining methods
Authors: Rodríguez-Hernandez, J.
Canut-Blasco, A.
Ledesma-Garcia, M.
Martín-Sánchez, A. M. 
UNESCO Clasification: 32 Ciencias médicas
3202 Epidemologia
Keywords: Cryptosporidium oocysts
Staining methods
Issue Date: 1994
Journal: European Journal of Epidemiology
Abstract: A study was carried out on the presence of oocysts ofCryptosporidium spp. in water for human consumption in the Province of Salamanca, Spain, using four different staining techniques: Ziehl-Neelsen, auramine, indirect immunofluorescence with monoclonal antibodies (Meridian, USA) and direct immunofluorescence with monoclonal antibodies (Pasteur, France). The presence of oocysts was detected in the water used for the washing of sand filters in the four water purifying plants and in the drinking water of two of the six geographical areas studied. The Ziehl-Neelsen and auramine staining techniques were found to have excellent diagnostic correlation. Immunofluorescence stainings with monoclonal antibodies proved to be less sensitive.
ISSN: 0392-2990
DOI: 10.1007/BF01730373
Source: European Journal of Epidemiology[ISSN 0392-2990],v. 10, p. 215-218 (Abril 1994)
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