Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/51113
DC FieldValueLanguage
dc.contributor.authorRemuzgo-Martínez, Sara
dc.contributor.authorLázaro-Díez, María
dc.contributor.authorMayer, Celia
dc.contributor.authorAranzamendi-Zaldumbide, Maitane
dc.contributor.authorPadilla, Daniel
dc.contributor.authorCalvo, Jorge
dc.contributor.authorMarco, Francesc
dc.contributor.authorMartínez-Martínez, Luis
dc.contributor.authorIcardo, José Manuel
dc.contributor.authorOtero, Ana
dc.contributor.authorRamos-Vivasa, José
dc.date.accessioned2018-11-24T21:34:18Z-
dc.date.available2018-11-24T21:34:18Z-
dc.date.issued2015
dc.identifier.issn0099-2240
dc.identifier.urihttp://hdl.handle.net/10553/51113-
dc.description.abstractSerratia spp. are opportunistic human pathogens responsible for an increasing number of nosocomial infections. However, little is known about the virulence factors and regulatory circuits that may enhance the establishment and long-term survival of Serratia liquefaciens in the hospital environment. In this study, two reporter strains, Chromobacterium violaceum CV026 and VIR24, and high-resolution triple-quadrupole liquid chromatography-mass spectrometry (LC-MS) were used to detect and to quantify N-acyl-homoserine lactone (AHL) quorum-sensing signals in 20 S. liquefaciens strains isolated from clinical samples. Only four of the strains produced sufficient amounts of AHLs to activate the sensors. Investigation of two of the positive strains by high-performance liquid chromatography (HPLC)-MS confirmed the presence of significant amounts of short-acyl-chain AHLs (N-butyryl-L-homoserine lactone [C-4-HSL] and N-hexanoyl-L-homoserine lactone [C-6-HSL]) in both strains, which exhibited a complex and strain-specific signal profile that included minor amounts of other short-acyl-chain AHLs (N-octanoyl-L-homoserine lactone [C-8-HSL] and N-3-oxohexanoyl-L-homoserine lactone [OC6-HSL]) and long-acyl-chain (C-10, C-12, and C-14) AHLs. No correlation between biofilm formation and the production of large amounts of AHLs could be established. Fimbria-like structures were observed by transmission electron microscopy, and the presence of the type 1 fimbrial adhesin gene fimH in all strains was confirmed by PCR. The ability of S. liquefaciens to adhere to abiotic surfaces and to form biofilms likely contributes to its persistence in the hospital environment, increasing the probability of causing nosocomial infections. Therefore, a better understanding of the adherence properties of this species will provide greater insights into the diseases it causes.
dc.publisher0099-2240
dc.relation.ispartofApplied and Environmental Microbiology
dc.sourceApplied and Environmental Microbiology[ISSN 0099-2240],v. 81, p. 3306-3315
dc.subject.otherBlood-Stream Infections
dc.subject.otherHafnia-Alvei Strains
dc.subject.otherIntensive-Care-Unit
dc.subject.otherPhase Variation
dc.subject.otherEscherichia-Coli
dc.subject.otherType-1 Fimbriae
dc.subject.otherMarcescens
dc.subject.otherVirulence
dc.subject.otherSurfaces
dc.subject.otherLactone
dc.titleBiofilm formation and quorum-sensing-molecule production by clinical isolates of Serratia liquefaciens
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1128/AEM.00088-15
dc.identifier.scopus84930027522
dc.identifier.isi000353336900004
dc.contributor.authorscopusid47861384300
dc.contributor.authorscopusid56407204500
dc.contributor.authorscopusid56117039000
dc.contributor.authorscopusid55256199000
dc.contributor.authorscopusid7005088705
dc.contributor.authorscopusid34871515700
dc.contributor.authorscopusid7005493096
dc.contributor.authorscopusid57207756829
dc.contributor.authorscopusid7005458506
dc.contributor.authorscopusid7004311013
dc.contributor.authorscopusid7102264071
dc.contributor.authorscopusid56659383500
dc.description.lastpage3315
dc.description.firstpage3306
dc.relation.volume81
dc.type2Artículoes
dc.contributor.daisngid1031649
dc.contributor.daisngid32598602
dc.contributor.daisngid6304881
dc.contributor.daisngid8037254
dc.contributor.daisngid1039753
dc.contributor.daisngid5097096
dc.contributor.daisngid59119
dc.contributor.daisngid67474
dc.contributor.daisngid428306
dc.contributor.daisngid1115739
dc.contributor.daisngid1854108
dc.contributor.wosstandardWOS:Remuzgo-Martinez, S
dc.contributor.wosstandardWOS:Lazaro-Diez, M
dc.contributor.wosstandardWOS:Mayer, C
dc.contributor.wosstandardWOS:Aranzamendi-Zaldumbide, M
dc.contributor.wosstandardWOS:Padilla, D
dc.contributor.wosstandardWOS:Calvo, J
dc.contributor.wosstandardWOS:Marco, F
dc.contributor.wosstandardWOS:Martinez-Martinez, L
dc.contributor.wosstandardWOS:Icardo, JM
dc.contributor.wosstandardWOS:Otero, A
dc.contributor.wosstandardWOS:Ramos-Vivas, J
dc.date.coverdateEnero 2015
dc.identifier.ulpgces
dc.description.sjr1,896
dc.description.jcr3,823
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR IUSA-ONE HEALTH 2 - Sanidad Animal de la Acuicultura y Especies Silvestres, Enfermedades Infecciosas y Seguridad Alimentaria-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0002-6678-5029-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNamePadilla Castillo, Daniel Fermín-
Appears in Collections:Artículos
Show simple item record

SCOPUSTM   
Citations

41
checked on Dec 8, 2024

WEB OF SCIENCETM
Citations

37
checked on Dec 8, 2024

Page view(s)

85
checked on Jun 29, 2024

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.