Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/50864
DC FieldValueLanguage
dc.contributor.authorRoy, Sylvie O.en_US
dc.contributor.authorPackard, Ted T.en_US
dc.date.accessioned2018-11-24T19:28:44Z-
dc.date.available2018-11-24T19:28:44Z-
dc.date.issued1998en_US
dc.identifier.issn0099-2240en_US
dc.identifier.urihttp://hdl.handle.net/10553/50864-
dc.description.abstractVariations of intracellular concentrations of isocitrate and NADP+ were measured throughout all growth phases of the marine bacterium Pseudomonas nautica. The intracellular isocitrate concentration tracked the intracellular protein concentration throughout all phases of growth. It rapidly increased in early exponential phase to a maximum and fell to nearly zero in parallel with pyruvate exhaustion in the culture medium. The intracellular NADP+ and protein concentrations increased in parallel during the exponential phase but were poorly correlated. Even after carbon exhaustion, the intracellular NADP+concentration stayed high, as did protein levels. The results demonstrated that the intracellular isocitrate concentration, but not the intracellular NADP+ concentration, was affected by the carbon availability in the culture. They also suggest that, because of its variability, isocitrate, but not NADP+, plays the larger role in the control of the respiratory CO2production rate (R CO2). From initial rate studies, bisubstrate Michaelis constants and the dissociation constant were determined for NADP+-specific isocitrate dehydrogenase (IDH) from P. nautica. These studies support the hypothesis that the mechanism of IDH’s activity involves the ordered addition of the substrates,d-isocitrate and NADP+. Furthermore, the results support the use of a bisubstrate enzyme kinetic equation to model R CO2 in P. nautica.en_US
dc.languageengen_US
dc.publisher0099-2240-
dc.relation.ispartofApplied and Environmental Microbiologyen_US
dc.sourceApplied and Environmental Microbiology [ISSN 0099-2240], v. 64, p. 4958-4964en_US
dc.subject251001 Oceanografía biológicaen_US
dc.subject2302 Bioquímicaen_US
dc.subject.otherIsocitrate Dehydrogenaseen_US
dc.subject.otherPseudomonasen_US
dc.subject.otherIsocitratesen_US
dc.subject.otherKineticsen_US
dc.subject.otherNADPen_US
dc.subject.otherPyruvatesen_US
dc.subject.otherSeawateren_US
dc.titleNADP-isocitrate dehydrogenase from Pseudomonas nautica: Kinetic constant determination and carbon limitation effects on the pool of intracellular substratesen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1128/AEM.64.12.4958-4964.1998en_US
dc.identifier.scopus0031764381-
dc.contributor.authorscopusid8923658500-
dc.contributor.authorscopusid7004249480-
dc.description.lastpage4964-
dc.description.firstpage4958-
dc.relation.volume64-
dc.investigacionCienciasen_US
dc.type2Artículoen_US
dc.utils.revisionen_US
dc.identifier.ulpgces
dc.description.jcr3,358
dc.description.jcrqQ1
dc.description.scieSCIE
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.orcid0000-0002-5880-1199-
crisitem.author.fullNamePackard, Theodore Train-
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