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Title: Impact of acetate, pyruvate, and physiological state on respiration and respiratory quotients in Pseudomonas nautica
Authors: Roy, Sylvie O.
Packard, Ted T. 
Berdalet, Elisa
St-Amand, Liliane
UNESCO Clasification: 251001 Oceanografía biológica
Keywords: CO2 production rates
O2 consumption rates
Isocitrate dehydrogenase
Respiratory electron transfer system
ETS, et al
Issue Date: 1999
Publisher: 0948-3055
Journal: Aquatic Microbial Ecology 
Abstract: Physiological rates of CO2 production and O2 consumption, and the activities of isocitrate dehydrogenase (IDH) and the electron transfer system (ETS) were studied in the marine bacterium Pseudomonas nautica growing on acetate. In exponential growth, IDH and ETS activities were well coupled with CO2 production and O2 consumption rates, but in senescence they were uncoupled. Our results clearly show that under starvation conditions, IDH and ETS activities remained high even though their corresponding respiration rates dropped. The respiratory metabolism in the different physiological states of the acetate-grown cultures was compared with previous observations made in pyruvate-grown cultures. Time profiles of CO2 production and O2 consumption rates showed completely different respiratory fingerprints associated with the different carbon sources. Acetate-grown cultures showed an increase of respiratory quotients (RQ) in the senescence phase whereas in pyruvate-grown cultures it stayed close to 1.0. On both carbon sources, respiration to respiratory capacity ratios were constant in exponential phase and decreased to almost zero after carbon source exhaustion. Our results clearly show the impact of physiological state and carbon sources on bacterial respiration rates
ISSN: 0948-3055
DOI: 10.3354/ame017105
Source: Aquatic Microbial Ecology [ISSN 0948-3055], v. 17, p. 105-110
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