Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/50831
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dc.contributor.authorRuiz De Galarreta, C. M.en_US
dc.contributor.authorFanjul, L. F.en_US
dc.contributor.authorMeidan, R.en_US
dc.contributor.authorHsueh, A. J.W.en_US
dc.date.accessioned2018-11-24T19:12:45Z-
dc.date.available2018-11-24T19:12:45Z-
dc.date.issued1983en_US
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/10553/50831-
dc.description.abstractdelta 5-3 beta-Hydroxysteroid dehydrogenase is a key enzyme for testicular androgen biosynthesis and a marker for the Leydig cells. The hormonal regulation of this enzyme was studied in cultured rat testicular cells. Human chorionic gonadotropin (hCG) increased testosterone production in vitro while time course studies indicated a biphasic action of the gonadotropin on 3 beta-hydroxysteroid dehydrogenase activity. An initial stimulation (51%) of the enzyme was detected between 3 and 12 h of culture when medium testosterone was low. This is followed by an inhibition of 3 beta-hydroxysteroid dehydrogenase activity on days 2 and 3 of culture when medium testosterone was elevated. Concomitant treatment with a synthetic androgen (R1881) inhibited 3 beta-hydroxysteroid dehydrogenase activity and testosterone production in hCG-treated cultures while an anti-androgen (cyproterone acetate) increased 3 beta-hydroxysteroid dehydrogenase activity and testosterone biosynthesis. Addition of 10(-5) M spironolactone, an inhibitor of 17 alpha-hydroxylase, blocked the hCG stimulation of testosterone production but increased medium progesterone. In the absence of the secreted androgen, hCG stimulated 3 beta-hydroxysteroid dehydrogenase activity in a time- and dose-related manner. Furthermore, hCG stimulation of 3 beta-hydroxysteroid dehydrogenase activity and progesterone accumulation in spironolactone-supplemented cultures was decreased by concomitant treatment with R1881 but was not affected by cyproterone acetate. The inhibitory effect of R1881 was blocked by the anti-androgen. In the absence of hCG, treatment with testosterone, dihydrotestosterone, or R1881, but not promegestone, alone also inhibited 3 beta-hydroxysteroid dehydrogenase activity while the inhibitory effect of testosterone was blocked by cyproterone acetate. Thus, hCG stimulates 3 beta-hydroxysteroid dehydrogenase activity in cultured testicular cells. The androgenic steroidogenic end products, in turn, inhibit this enzyme. The hormonal regulation of 3 beta-hydroxysteroid dehydrogenase activity may be important in the ultrashort loop autoregulation of androgen biosynthesis.en_US
dc.languageengen_US
dc.publisher0021-9258
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.sourceJournal of Biological Chemistry[ISSN 0021-9258],v. 258, p. 10988-10996en_US
dc.subject2407 Biología celularen_US
dc.subject2411 Fisiología humanaen_US
dc.subject.otherCultured Testicular Cellsen_US
dc.subject.otherHormonal regulationen_US
dc.titleRegulation of 3β-hydroxysteroid dehydrogenase activity by human chorionic gonadotropin, androgens, and anti-androgens in cultured testicular cellsen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.scopus0020518757-
dc.contributor.authorscopusid7003806034-
dc.contributor.authorscopusid7004158812-
dc.contributor.authorscopusid7004843040-
dc.contributor.authorscopusid56262915100-
dc.description.lastpage10996en_US
dc.description.firstpage10988en_US
dc.relation.volume258en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.utils.revisionen_US
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética E Inmunología.-
crisitem.author.deptSeñalización Intracelular y Expresión Génica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameFanjul Rodríguez, Luisa Fernanda-
crisitem.author.departamentoBioquímica y Biología Molecular, Fisiología, Genética E Inmunología.-
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