Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/50603
Título: Partial characterization of retroviruses from boid snakes with inclusion body disease
Autores/as: Jacobson, Elliott R.
Orós, Jorge 
Tucker, Sylvia J.
Pollock, David P.
Kelley, Karen L.
Munn, Robert J.
Lock, Brad A.
Mergia, Ayalew
Yamamoto, Janet K.
Clasificación UNESCO: 32 Ciencias médicas
3205 Medicina interna
Palabras clave: Virus
Islands
Fecha de publicación: 2001
Editor/a: 0002-9645
Publicación seriada: American journal of veterinary research 
Resumen: Objective-To characterize retroviruses isolated from bold snakes with inclusion body disease (IBD).Animals-2 boa constrictors with IBD and 1 boa exposed to an affected snake.Procedure-Snakes were euthanatized, and tissue specimens and blood samples were submitted for virus isolation. Tissue specimens were cultured with or without commercially available viper heart cells and examined by use of transmission electron microscopy (TEM) for evidence of viral replication. Reverse transcriptase activity was determined ii? sucrose gradient-purified virus. Western blotting was performed, using polyclonal antibodies against 1 of the isolated viruses. Specificity of the rabbit anti-virus antibody was evaluated, using an immunogold-labeling TEM technique.Results-3 viruses (RV-1. RV-2, and RV-3) were isolated. The isolates were morphologically comparable to members of the Retroviridae family. Reverse transcriptase activity was high in sucrose gradient fractions that were rich in virus. Polyclonal antibody against RV-1 reacted with proteins of similar relative mobility in RV-1 and RV-2. By use of immunogold labeling, this antibody also recognized virions of both RV-1 and RV-2.Conclusions and Clinical Relevance-A retrovirus was isolated from bold snakes with IBD or exposed to IBD. Western blot analysis of viral proteins indicated that viruses isolated from the different snakes were similar. Whether this virus represents the causative agent of IBD is yet to be determined. The isolation of retroviruses from bold snakes with IBD is an important step in the process of identifying the causative agent of this disease.
URI: http://hdl.handle.net/10553/50603
ISSN: 0002-9645
DOI: 10.2460/ajvr.2001.62.217
Fuente: American Journal of Veterinary Research[ISSN 0002-9645],v. 62, p. 217-224 (Enero 2001)
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