Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/50581
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dc.contributor.authorReyes, Juan González
dc.contributor.authorRobayna, Ignacio González
dc.contributor.authorDelgado, Pino Santana
dc.contributor.authorGonzález, Inmaculada Hernández
dc.contributor.authorAguiar, José Quintana
dc.contributor.authorEstévez Rosas, F.
dc.contributor.authorFanjul, Luisa F.
dc.contributor.authorRuiz de Galarreta, C. M.
dc.contributor.otherQuintana, Jose
dc.contributor.otherGonzalez Robayna, Ignacio
dc.contributor.otherEstevez, Francisco
dc.date.accessioned2018-11-24T17:10:00Z-
dc.date.available2018-11-24T17:10:00Z-
dc.date.issued1996
dc.identifier.issn0021-9258
dc.identifier.urihttp://hdl.handle.net/10553/50581-
dc.description.abstractStimulation of [H-3]serine-labeled A431 cells with tumor necrosis factor-alpha (TNF alpha) or bacterial sphingomyelinase (SMase) resulted in a rapid decrease (similar to 50% by 15 min) in cellular [H-3]sphingomyelin content and generation of the lipid moiety [H-3]ceramide, which remained elevated 60 min later, Sphingomyelin hydrolysis in response to TNF alpha or bacterial SMase resulted in a time-dependent decrease in the phosphorylation state of c-Jun protein, an effect that was also observed in cells treated with the membrane-permeable ceramide analogue N-hexanoylsphingosine (C-6-ceramide). The rapid dephosphorylation of the c-Jun gene product in response to TNF alpha, SMase, or C-6-ceramide was not observed in A431 cells treated with the serine-threonine phosphatase inhibitor okadaic acid. After the initial steps of previously described methods for the purification of a ceramide-activated protein phosphatase termed CAPP (Dobrowsky, R, T., Kamibayashi, C,, Mumby, M, C., and Hannun, Y. A, (1993) J, Biol, Chen. 268, 15523-15530), we obtained a cytosolic fraction from A431 cells that specifically dephosphorylated P-32(i)-labeled c-Jun protein used as substrate in an immunocomplex phosphatase assay, Phosphatase activity in vitro was apparent only in the presence of ceramide (5 mu m) and was specifically abrogated when okadaic acid (1 nM) was included in the immunocomplex phosphatase assay, These results provide strong evidence for c-Jun as a downstream target for CAPP activated in response to post-TNF signaling in A431 cells.
dc.publisher0021-9258
dc.relation.ispartofJournal of Biological Chemistry
dc.sourceJournal Of Biological Chemistry[ISSN 0021-9258],v. 271 (35), p. 21375-21380
dc.subject.otherTumor-Necrosis-Factor
dc.subject.otherSphingolipid Breakdown Products
dc.subject.otherSignal-Transduction
dc.subject.otherCellular-Regulation
dc.subject.otherFactor-Alpha
dc.subject.otherHa-Ras
dc.subject.otherNeutral Sphingomyelinase
dc.subject.otherGranulosa-Cells
dc.subject.otherKinase-Activity
dc.subject.otherPhorbol Ester
dc.titlec-Jun is a downstream target for ceramide-activated protein phosphatase in A431 cells
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1074/jbc.271.35.21375
dc.identifier.scopus0029834974
dc.identifier.isiA1996VE47700064
dcterms.isPartOfJournal Of Biological Chemistry
dcterms.sourceJournal Of Biological Chemistry[ISSN 0021-9258],v. 271 (35), p. 21375-21380
dc.contributor.authorscopusid16167408800
dc.contributor.authorscopusid6507425244
dc.contributor.authorscopusid6507425244
dc.contributor.authorscopusid7006357982
dc.contributor.authorscopusid57196683525
dc.contributor.authorscopusid7005928829
dc.contributor.authorscopusid7003810011
dc.contributor.authorscopusid7004158812
dc.contributor.authorscopusid7003806034
dc.description.lastpage21380
dc.description.firstpage21375
dc.relation.volume271
dc.type2Artículoes
dc.identifier.wosWOS:A1996VE47700064
dc.contributor.daisngid369809
dc.contributor.daisngid23157769
dc.contributor.daisngid34195916
dc.contributor.daisngid4535243
dc.contributor.daisngid4055694
dc.contributor.daisngid2922182
dc.contributor.daisngid6712290
dc.contributor.daisngid4633590
dc.contributor.daisngid218599
dc.contributor.daisngid1127140
dc.contributor.daisngid1664323
dc.identifier.investigatorRIDK-5709-2014
dc.identifier.investigatorRIDK-9671-2014
dc.identifier.investigatorRIDK-5125-2014
dc.contributor.wosstandardWOS:Reyes, JG
dc.contributor.wosstandardWOS:Robayna, IG
dc.contributor.wosstandardWOS:Delgado, PS
dc.contributor.wosstandardWOS:Gonzalez, IH
dc.contributor.wosstandardWOS:Aguiar, JQ
dc.contributor.wosstandardWOS:Rosas, FE
dc.contributor.wosstandardWOS:Fanjul, LF
dc.contributor.wosstandardWOS:deGalarreta, CMR
dc.date.coverdateSeptiembre 1996
dc.identifier.ulpgces
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptSeñalización Intracelular y Expresión Génica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptSeñalización Intracelular y Expresión Génica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptSeñalización Intracelular y Expresión Génica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptBioquímica Farmacológica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptBioquímica Farmacológica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptSeñalización Intracelular y Expresión Génica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.orcid0000-0002-7650-4454-
crisitem.author.orcid0000-0002-4093-2692-
crisitem.author.orcid0000-0002-7650-4454-
crisitem.author.orcid0000-0001-8225-4538-
crisitem.author.orcid0000-0002-9728-2774-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameGonzález Robayna, Ignacio Javier-
crisitem.author.fullNameSantana Delgado, María Del Pino-
crisitem.author.fullNameGonzález Robayna, Ignacio Javier-
crisitem.author.fullNameQuintana Aguiar, José Martín-
crisitem.author.fullNameEstévez Rosas, Francisco Jesús-
crisitem.author.fullNameFanjul Rodríguez, Luisa Fernanda-
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