Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/50222
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dc.contributor.authorAyuso, Rosalíaen_US
dc.contributor.authorSánchez-Garcia, Silviaen_US
dc.contributor.authorLin, Jingen_US
dc.contributor.authorFu, Zhiyanen_US
dc.contributor.authorIbáñez, María Doloresen_US
dc.contributor.authorCarrillo, Teresaen_US
dc.contributor.authorBlanco, Carlosen_US
dc.contributor.authorGoldis, Marinaen_US
dc.contributor.authorBardina, Ludmilaen_US
dc.contributor.authorSastre, Joaquínen_US
dc.contributor.authorSampson, Hugh A.en_US
dc.date.accessioned2018-11-24T14:21:18Z-
dc.date.available2018-11-24T14:21:18Z-
dc.date.issued2010en_US
dc.identifier.issn0091-6749en_US
dc.identifier.urihttp://hdl.handle.net/10553/50222-
dc.description.abstractBackground: Shellfish allergy is a long-lasting disorder typically affecting adults. Despite its high prevalence, there is limited information about allergenic shrimp proteins and the epitopes implicated in such allergic reactions. Objective: We sought to identify the IgE-binding epitopes of the 4 shrimp allergens and to characterize epitope recognition profiles of children and adults with shrimp allergy. Methods: Fifty-three subjects, 34 children and 19 adults, were selected with immediate allergic reactions to shrimp, increased shrimp-specific serum IgE levels, and positive immunoblot binding to shrimp. Study subjects and 7 nonatopic control subjects were tested by means of peptide microarray for IgE binding with synthetic overlapping peptides spanning the sequences of Litopenaeus vannamei shrimp tropomyosin, arginine kinase (AK), myosin light chain (MLC), and sarcoplasmic calcium-binding protein (SCP). The Wilcoxon test was used to determine significant differences in z scores between patients and control subjects. Results: The median shrimp IgE level was 4-fold higher in children than in adults (47 vs 12.5 kU(A)/L). The frequency of allergen recognition was higher in children (tropomyosin, 81% [94% for children and 61% for adults]; MLC, 57% [70% for children and 31% for adults]; AK, 51% [67% for children and 21% for adults]; and SCP, 45% [59% for children and 21% for adults]), whereas control subjects showed negligible binding. Seven IgE-binding regions were identified in tropomyosin by means of peptide microarray, confirming previously identified shrimp epitopes. In addition, 3 new epitopes were identified in tropomyosin (epitopes 1, 3, and 5b-c), 5 epitopes were identified in MLC, 3 epitopes were identified in SCP, and 7 epitopes were identified in AK. Interestingly, frequency of individual epitope recognition, as well as intensity of IgE binding, was significantly greater in children than in adults for all 4 proteins. Conclusions: Children with shrimp allergy have greater shrimp-specific IgE antibody levels and show more intense binding to shrimp peptides and greater epitope diversity than adults.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Allergy and Clinical Immunologyen_US
dc.sourceJournal of Allergy and Clinical Immunology[ISSN 0091-6749],v. 125(6), pp. 1286-1293.e3 (Junio 2010)en_US
dc.subject32 Ciencias médicasen_US
dc.subject320701 Alergiasen_US
dc.subject.otherAllergensen_US
dc.subject.otherArginine kinaseen_US
dc.subject.otherEpitopesen_US
dc.titleGreater epitope recognition of shrimp allergens by children than by adults suggests that shrimp sensitization decreases with ageen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.jaci.2010.03.010en_US
dc.identifier.scopus77952742360-
dc.contributor.authorscopusid7004311545-
dc.contributor.authorscopusid26639678200-
dc.contributor.authorscopusid56221944600-
dc.contributor.authorscopusid34971103200-
dc.contributor.authorscopusid24444733200-
dc.contributor.authorscopusid7003526269-
dc.contributor.authorscopusid57189070218-
dc.contributor.authorscopusid35810109700-
dc.contributor.authorscopusid6701363147-
dc.contributor.authorscopusid7102442750-
dc.contributor.authorscopusid16742237000-
dc.description.lastpage1293en_US
dc.description.firstpage1286en_US
dc.relation.volume125en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.description.numberofpages8en_US
dc.utils.revisionen_US
dc.date.coverdateJunio 2010en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.jcr9,273-
dc.description.jcrqQ1-
dc.description.scieSCIE-
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR IUIBS: Patología y Tecnología médica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Médicas y Quirúrgicas-
crisitem.author.orcid0000-0002-3047-8908-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameCarrillo Díaz, Teresa-
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