Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/50049
Campo DC Valoridioma
dc.contributor.authorArguello, A
dc.contributor.authorLópez-Fernández, Juan Luis
dc.contributor.authorRivero, Joseuis L.
dc.contributor.otherArguello, Anastasio
dc.date.accessioned2018-11-24T12:51:31Z-
dc.date.available2018-11-24T12:51:31Z-
dc.date.issued2001
dc.identifier.issn0003-276X
dc.identifier.urihttp://hdl.handle.net/10553/50049-
dc.description.abstractThe primary focus of this study was the accurate classification of limb skeletal muscle fiber types in adult goats (Capra hircus) according to the myosin heavy chain (MHC) isoform they express. Combined methodologies of gel electrophoresis, immunoblotting, immunohistochemistry, myofibrillar ATPase (mATPase), and quantitative metabolic enzyme histochemistry of M. semitendinosus samples were developed. Three MHCs were identified and tentatively designated as types I, IIA, and IIX. Five fiber types were defined immunohistochemically according to their MHC content: I, I + IIA, IIA, IIAX, and IIX. The hybrid fast-twitch fibers (IIAX) totaled 21% of the fiber population analyzed. The three major pure fibers (I, IIA, and IIX) could be objectively separated upon the basis of their mATPase activities after acid and alkaline preincubations. The prominent number of hybrid fibers, however, could not be delineated with these mATPase methods. Metabolic and size properties of muscle fibers varied according to their MHC content, but overlapped the full range of muscle fiber phenotypes. These integrated data demonstrate that type II skeletal muscle fibers of small ruminants have been misclassified in previous studies. The immunohistochemical approach developed in the present study offers new prospects for muscle fiber typing in caprine experimental studies and meat production technologies. Anat Rec 264:284-293, 2001. (C) 2001 Wiley-Liss, Inc.
dc.publisher0003-276X
dc.relation.ispartofThe anatomical record (Print)
dc.sourceAnatomical Record[ISSN 0003-276X],v. 264 (3), p. 284-293
dc.subject.otherRat Skeletal-Muscle
dc.subject.otherMonoclonal-Antibodies
dc.subject.otherBovine Muscle
dc.subject.otherIsoforms
dc.subject.otherHistochemistry
dc.subject.otherIdentification
dc.subject.otherPopulation
dc.subject.otherProtein
dc.subject.otherAtpase
dc.subject.otherSheep
dc.titleLimb myosin heavy chain isoproteins and muscle fiber types in the adult goat (Capra hircus)
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1002/ar.1165
dc.identifier.scopus0035499035
dc.identifier.isi000171962700006
dcterms.isPartOfAnatomical Record
dcterms.sourceAnatomical Record[ISSN 0003-276X],v. 264 (3), p. 284-293
dc.contributor.authorscopusid6701710018
dc.contributor.authorscopusid23106588200
dc.contributor.authorscopusid7006478807
dc.description.lastpage293
dc.description.firstpage284
dc.relation.volume264
dc.type2Artículoes
dc.identifier.wosWOS:000171962700006
dc.contributor.daisngid298051
dc.contributor.daisngid25873248
dc.contributor.daisngid2608566
dc.contributor.daisngid1820232
dc.identifier.investigatorRIDB-4493-2010
dc.contributor.wosstandardWOS:Arguello, A
dc.contributor.wosstandardWOS:Lopez-Fernandez, JL
dc.contributor.wosstandardWOS:Rivero, JLL
dc.date.coverdateNoviembre 2001
dc.identifier.ulpgces
dc.description.jcr1,444
dc.description.jcrqQ2
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IUSA-ONEHEALTH 4. Producción y Biotecnología Animal-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0002-4426-0678-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameArgüello Henríquez, Anastasio-
Colección:Artículos
Vista resumida

Citas SCOPUSTM   

47
actualizado el 24-mar-2024

Citas de WEB OF SCIENCETM
Citations

48
actualizado el 25-feb-2024

Visitas

43
actualizado el 21-oct-2023

Google ScholarTM

Verifica

Altmetric


Comparte



Exporta metadatos



Los elementos en ULPGC accedaCRIS están protegidos por derechos de autor con todos los derechos reservados, a menos que se indique lo contrario.