Overexpression system and biochemical profile of CTX-M-3 extended-spectrum β-lactamase expressed in Escherichia coli

Title:	Overexpression system and biochemical profile of CTX-M-3 extended-spectrum β-lactamase expressed in Escherichia coli
Authors:	Perilli, Mariagrazia Ettorre, Daniela Segatore, Bernardetta Caporale, Bibiana Santis, Francesca De Pochetti, Giorgio Brigante, Gioconda Mazza, Fernando Tavìo, Marìa M. Amicosante, Gianfranco
UNESCO Clasification:	32 Ciencias médicas 320103 Microbiología clínica
Keywords:	Klebsiella-Pneumoniae Enterobacteriaceae Enzyme Identification Proteins, et al
Issue Date:	2004
Journal:	FEMS Microbiology Letters
Abstract:	An efficient over-expression system was developed for CTX-M-3 extended-spectrum-beta-lactamase. The recombinant enzyme was purified from 11 of culture to yield 22 mg of pure enzyme. The N-terminal amino acid sequence was determined to be NH2-QTADVQ... Determination of kinetic parameters with the purified CTX-M-3 revealed efficient hydrolysis of penicillins and cephalosporins, while ceftazidime and aztreonam were very poor substrates. Clavulanic acid, sulbactam and especially tazobactam inhibited the CTX-M-3 enzyme.
URI:	http://hdl.handle.net/10553/49969
ISSN:	0378-1097
DOI:	10.1016/j.femsle.2004.10.031
Source:	FEMS Microbiology Letters[ISSN 0378-1097],v. 241, p. 229-232
Appears in Collections:	Artículos

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