Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/49962
Campo DC Valoridioma
dc.contributor.authorFàbrega, Anna
dc.contributor.authorMartin, Robert G.
dc.contributor.authorRosner, Judah L.
dc.contributor.authorTavio, M. Mar
dc.contributor.authorVila, Jordi
dc.date.accessioned2018-11-24T12:09:02Z-
dc.date.available2018-11-24T12:09:02Z-
dc.date.issued2010
dc.identifier.issn0066-4804
dc.identifier.urihttp://hdl.handle.net/10553/49962-
dc.description.abstractElevated levels of fluoroquinolone resistance are frequently found among Escherichia coli clinical isolates. This study investigated the antibiotic resistance mechanisms of strain NorE5, derived in vitro by exposing an E. coli clinical isolate, PS5, to two selection steps with increasing concentrations of norfloxacin. In addition to the amino acid substitution in GyrA (S83L) present in PS5, NorE5 has an amino acid change in ParC (S80R). Furthermore, we now find by Western blotting that NorE5 has a multidrug resistance phenotype resulting from the overexpression of the antibiotic resistance efflux pump AcrAB-TolC. Microarray and gene fusion analyses revealed significantly increased expression in NorE5 of soxS, a transcriptional activator of acrAB and tolC. The high soxS activity is attributable to a frameshift mutation that truncates SoxR, rendering it a constitutive transcriptional activator of soxS. Furthermore, microarray and reverse transcription-PCR analyses showed that mdtG (yceE), encoding a putative efflux pump, is overexpressed in the resistant strain. SoxS, MarA, and Rob activated an mdtG::lacZ fusion, and SoxS was shown to bind to the mdtG promoter, showing that mdtG is a member of the marA-soxS-rob regulon. The mdtG marbox sequence is in the backward or class I orientation within the promoter, and its disruption resulted in a loss of inducibility by MarA, SoxS, and Rob. Thus, chromosomal mutations in parC and soxR are responsible for the increased antibiotic resistance of NorE5.
dc.publisher0066-4804
dc.relation.ispartofAntimicrobial Agents and Chemotherapy
dc.sourceAntimicrobial Agents and Chemotherapy[ISSN 0066-4804],v. 54, p. 1218-1225
dc.subject.otherMultiple-Antibiotic-Resistance
dc.subject.otherEscherichia-Coli Strains
dc.subject.otherUrinary-Tract-Infections
dc.subject.otherTranscriptional Activator
dc.subject.otherEfflux Pump
dc.subject.otherCiprofloxacin Resistance
dc.subject.otherQuinolone Resistance
dc.subject.otherMutations
dc.subject.otherGenes
dc.subject.otherAcrab
dc.titleConstitutive SoxS expression in a fluoroquinolone-resistant strain with a truncated SoxR protein and identification of a new member of the marA-soxS-rob regulon, mdtG
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1128/AAC.00944-09
dc.identifier.scopus77149160578
dc.identifier.isi000274733300035
dc.contributor.authorscopusid23977696300
dc.contributor.authorscopusid7501380895
dc.contributor.authorscopusid7101710678
dc.contributor.authorscopusid6701659492
dc.contributor.authorscopusid7202012753
dc.description.lastpage1225
dc.description.firstpage1218
dc.relation.volume54
dc.type2Artículoes
dc.contributor.daisngid858051
dc.contributor.daisngid1282603
dc.contributor.daisngid1431319
dc.contributor.daisngid7853284
dc.contributor.daisngid24031
dc.contributor.wosstandardWOS:Fabrega, A
dc.contributor.wosstandardWOS:Martin, RG
dc.contributor.wosstandardWOS:Rosner, JL
dc.contributor.wosstandardWOS:Tavio, MM
dc.contributor.wosstandardWOS:Vila, J
dc.date.coverdateMarzo 2010
dc.identifier.ulpgces
dc.description.jcr4,672
dc.description.jcrqQ1
dc.description.scieSCIE
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR Investigación Básica y Aplicada en Ciencias de la Salud-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.orcid0000-0002-1808-7461-
crisitem.author.parentorgDepartamento de Ciencias Clínicas-
crisitem.author.fullNameTavío Pérez, María Del Mar-
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