Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/49388
Campo DC Valoridioma
dc.contributor.authorFerre-López, Sergioen_US
dc.contributor.authorRibes-Koninckx, Carmenen_US
dc.contributor.authorGenzor, Carlosen_US
dc.contributor.authorGamen, Susanaen_US
dc.contributor.authorPeña Quintana, Luisen_US
dc.contributor.authorOrtigosa, Luisen_US
dc.contributor.authorMéndez, Enriqueen_US
dc.date.accessioned2018-11-24T06:57:33Z-
dc.date.available2018-11-24T06:57:33Z-
dc.date.issued2004en_US
dc.identifier.issn1542-3565en_US
dc.identifier.urihttp://hdl.handle.net/10553/49388-
dc.description.abstractBackground & Aims: We investigated two 1-step immunochromatographic visual assays based on human recombinant tissue-transglutaminase (t-TG) as an alternative to enzyme-linked immunosorbent assays (ELISAs) for celiac disease (CD) screening. Methods: We used a tissue-transglutaminase (t-TG) stick, which detected immunoglobulin A/G (IgA/G) antibodies to t-TG, and a t-TG/antigliadin antibodies (AGA) stick, which detected IgA antibodies to both t-TG and AGA, as well as t-TG and AGA ELISAs, to determine t-TG and AGA antibodies in untreated celiac patients with subtotal villous atrophy. A total of 142 children (3 IgA-deficient sera) and 30 adults, and 140 controls (normal mucosa; 121 children and 19 adults), plus 23 sera from pediatric CD patients in remission were assayed. Results: For pediatric patients, with the t-TG stick we obtained a sensitivity of 97.1% and a specificity of 99.0%, and in adults, 83.3% and 100%, respectively. The t-TG/AGA stick displayed a sensitivity of 95.7% and a specificity of 99.0% for t-TG and a sensitivity of 89.2% and a specificity of 95.8% for AGA in children, and a sensitivity of 80% and a specificity of 100% for t-TG and a sensitivity of 83.3% and a specificity of 100% for AGA in adults. Results were comparable with the corresponding ELISAs. Conclusions: The 2 visual assays are efficient for CD screening as an alternative to ELISAs. They are simple to handle and to interpret. By the combined use of the 2 sticks, IgA-deficient patients can be identified as positive in the t-TG (IgG/A) but negative in the t-TG/AGA (IgA) stick.en_US
dc.languageengen_US
dc.relation.ispartofClinical Gastroenterology and Hepatologyen_US
dc.sourceClinical Gastroenterology and Hepatology[ISSN 1542-3565],v. 2(6), p. 480-484 (Junio 2004)en_US
dc.subject32 Ciencias médicasen_US
dc.subject320503 Gastroenterologíaen_US
dc.subject.otherAntigliadin antibodiesen_US
dc.subject.otherCeliac diseaseen_US
dc.subject.otherEnzyme-linked immunosorbent assayen_US
dc.subject.otherImmunoglobulinen_US
dc.titleImmunochromatographic sticks for tissue transglutaminase and antigliadin antibody screening in celiac diseaseen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/S1542-3565(04)00166-1en_US
dc.identifier.scopus2542551894-
dc.contributor.authorscopusid6506142991-
dc.contributor.authorscopusid6602108756-
dc.contributor.authorscopusid6603489285-
dc.contributor.authorscopusid57196153288-
dc.contributor.authorscopusid6603266503-
dc.contributor.authorscopusid54946469200-
dc.contributor.authorscopusid7102731729-
dc.description.lastpage484en_US
dc.identifier.issue6-
dc.description.firstpage480en_US
dc.relation.volume2en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.description.numberofpages5en_US
dc.utils.revisionen_US
dc.date.coverdateJunio 2004en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.scieSCIE-
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IUIBS: Nutrición-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.orcid0000-0001-6052-5894-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNamePeña Quintana, Luis-
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