Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/49146
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dc.contributor.authorFleitas, J. L.
dc.contributor.authorTejedor-Junco, M. T.
dc.contributor.authorSantana, A.
dc.contributor.authorAndrada, M.
dc.contributor.authorRamírez, A. S.
dc.contributor.otherRamirez, Ana
dc.contributor.otherTejedor-Junco, Maria Teresa
dc.date.accessioned2018-11-24T04:34:22Z-
dc.date.available2018-11-24T04:34:22Z-
dc.date.issued2015
dc.identifier.issn0921-4488
dc.identifier.urihttp://hdl.handle.net/10553/49146-
dc.description.abstractCoxiella (C.) burnetii is an obligate intracellular microorganism which causes Q fever, an important zoonotic disease. C burnetii can be excreted in birth products, feces, urine and milk. It is known that C. burnetii can be shed in milk during several lactation periods. The PCR based on the transposon-like element IS1111a genes target has been proved to be highly sensitive and specific. In this study we present an adaptation of the primer set designed for conventional PCR targeting the multicopy IS1111 repeat element to be used in real-time PCR (real time trans-PCR) and the design of a mathematical model for calculating the sensitivity of a real-time trans-PCR analysis for C burnetii in goat milk. The adaptation of the trans-PCR into real time was evaluated and positive goat milk samples were analyzed with it. With the aim of estimate the probability of false negative, we fit a probability distribution to the threshold cycle values. Several probability distributions have been considered. In all cases the goodness of fit has been assessed by using Kolmogorov-Smirnov and Anderson-Darling Quadratic tests. Using real time trans-PCR, it was possible to obtain a clear positive reading for 8.01 x 10(3) cells/ml. From the 56 milk samples tested, the real time trans-PCR assay revealed real positivity for C. burnetii in all of them. The logistic fit leads to an estimation of the rate of false negatives of 8.07%, which would have a sensitivity of 91.93% (using 41 cycles in the protocol). The primers for IS111 region described could be used and they are effective to detect C. burnetii in milk samples. (C) 2014 Elsevier B.V. All rights reserved.
dc.publisher0921-4488
dc.relation.ispartofSmall Ruminant Research
dc.sourceSmall Ruminant Research[ISSN 0921-4488],v. 123, p. 149-154
dc.subject.otherPolymerase-Chain-Reaction
dc.subject.otherBulk Tank Milk
dc.subject.otherQ-Fever
dc.subject.otherSamples
dc.subject.otherSeroprevalence
dc.subject.otherPrevalence
dc.subject.otherInfection
dc.subject.otherAgent
dc.subject.otherSpain
dc.subject.otherAssay
dc.titleMathematical method for calculating the sensitivity of a real-time trans-PCR analysis for Coxiella burnetii in goat milk
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1016/j.smallrumres.2014.09.011
dc.identifier.scopus84919372027
dc.identifier.isi000349271400023
dcterms.isPartOfSmall Ruminant Research
dcterms.sourceSmall Ruminant Research[ISSN 0921-4488],v. 123 (1), p. 149-154
dc.contributor.authorscopusid23967626000
dc.contributor.authorscopusid6603353402
dc.contributor.authorscopusid56554207500
dc.contributor.authorscopusid56176713700
dc.contributor.authorscopusid6602991535
dc.contributor.authorscopusid57214574565
dc.contributor.authorscopusid7401735183
dc.description.lastpage154
dc.description.firstpage149
dc.relation.volume123
dc.type2Artículoes
dc.identifier.wosWOS:000349271400023
dc.contributor.daisngid10824752
dc.contributor.daisngid2312733
dc.contributor.daisngid4213964
dc.contributor.daisngid862879
dc.contributor.daisngid1521915
dc.contributor.daisngid4403282
dc.identifier.investigatorRIDL-5255-2014
dc.identifier.investigatorRIDNo ID
dc.contributor.wosstandardWOS:Fleitas, JL
dc.contributor.wosstandardWOS:Tejedor-Junco, MT
dc.contributor.wosstandardWOS:Santana, A
dc.contributor.wosstandardWOS:Andrada, M
dc.contributor.wosstandardWOS:Ramirez, AS
dc.date.coverdateEnero 2015
dc.identifier.ulpgces
dc.description.sjr0,603
dc.description.jcr1,083
dc.description.sjrqQ2
dc.description.jcrqQ2
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptTrypanosomosis, Resistencia a Antibióticos y Medicina Animal-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptCiencias Clínicas-
crisitem.author.deptEstadística-
crisitem.author.deptMatemáticas-
crisitem.author.deptHistología y Patología Veterinaria-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptMorfología-
crisitem.author.deptEpidemiología y Medicina Preventiva Veterinaria-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptPatología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0003-2387-1426-
crisitem.author.orcid0000-0002-6513-4814-
crisitem.author.orcid0000-0002-2411-9714-
crisitem.author.orcid0000-0002-8721-775X-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgMatemáticas-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameTejedor Junco, María Teresa-
crisitem.author.fullNameSantana Del Pino, Ángelo-
crisitem.author.fullNameAndrada Borzollino, Marisa Ana-
crisitem.author.fullNameRamírez Corbera, Ana Sofía-
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