Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/48715
DC FieldValueLanguage
dc.contributor.authorNavarro Bosch, Domingoen_US
dc.contributor.authorDoreste, Hilarioen_US
dc.contributor.authorCabrera, Juan J.en_US
dc.contributor.authorMorales, Manuelen_US
dc.contributor.authorDiaz-Chico, JCen_US
dc.contributor.authorDíaz-Chico, Bonifacio N.en_US
dc.contributor.otherDiaz-Chico, Juan-
dc.contributor.otherCabrera-Galvan, Juan Jose-
dc.date.accessioned2018-11-24T00:18:25Z-
dc.date.available2018-11-24T00:18:25Z-
dc.date.issued1998en_US
dc.identifier.issn0167-6806en_US
dc.identifier.urihttp://hdl.handle.net/10553/48715-
dc.description.abstract[H-3]Tamoxifen Aziridine ([H-3]TAZ) is a derivative of the antiestrogen tamoxifen that covalently labels the Estrogen Receptor (ER), and perhaps other uncharacterized proteins. In a previous article we described that [H-3]TAZ binds to a cytosolic protein from human uterine tissues that shares some, but not all, the ER properties. Here we have extended these studies to [H-3]TAZ binding to cytosol proteins from human breast cancer specimens, and studied its quantitative association with other molecular markers and clinico-pathological variables. Cytosols were obtained in hypotonic buffer containing 20 mM molybdate and protease inhibitors, incubated with [H-3]TAZ, and subjected to Sucrose Gradient Analysis (SGA). A [H-3]TAZ labeled peak that consistently migrated with the 4S fractions was found in most of the assayed cytosols (range of 0 to 1278 fmol/mg p.). The 4S peak of [H-3]TAZ was partially inhibited by both estrogens and antiestrogens. When [H-3]E-2 was used instead of [H-3]TAZ, only an SS peak was detected. [H-3]TAZ was covalently bound to a protein with an apparent MW of 65 kDa, as determined by SDS-PAGE and fluorography. The mean of [H-3]TAZ binding was significantly higher in the subgroups of samples classified as ER-, PR-, pS2- or cathepsin D-, than in the respective positive subgroups (P < 0.01 in all the cases). [H-3]TAZ binding was not associated with clinicopathological variables, except that its mean was significantly larger in tumors larger than 5 cm than in smaller tumors. These results, and those previously reported, suggest that: 1) [H-3]TAZ labels a cytosolic protein present in human breast cancers and uterine tissues that does not share all the ER properties, and 2) the [H-3]TAZ binding by breast cancer cytosols is negatively associated with markers of estrogenic dependency, and its quantification may provide valuable information on antiestrogen responsiveness of a given tumor.en_US
dc.languageengen_US
dc.relation.ispartofBreast Cancer Research and Treatmenten_US
dc.sourceBreast Cancer Research And Treatment[ISSN 0167-6806],v. 50 (2), p. 155-166en_US
dc.subject32 Ciencias médicasen_US
dc.subject320101 Oncologíaen_US
dc.subject.otherHormone Receptorsen_US
dc.subject.otherCanceren_US
dc.subject.otherTumorsen_US
dc.subject.otherMechanismsen_US
dc.subject.otherResistanceen_US
dc.subject.otherEstradiolen_US
dc.subject.otherVarianten_US
dc.subject.otherSitesen_US
dc.subject.otherPhosphorylationen_US
dc.subject.otherAntiestrogensen_US
dc.titleTamoxifen aziridine binding to cytosolic proteins from human breast specimens is negatively associated with estrogen receptors, progesterone receptors, pS2, and cathepsin-Den_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1023/A:1006062510883en_US
dc.identifier.scopus0031683433-
dc.identifier.isi000076443700006-
dcterms.isPartOfBreast Cancer Research And Treatment-
dcterms.sourceBreast Cancer Research And Treatment[ISSN 0167-6806],v. 50 (2), p. 155-166-
dc.contributor.authorscopusid7004873659-
dc.contributor.authorscopusid6503922510-
dc.contributor.authorscopusid6602257053-
dc.contributor.authorscopusid36456076700-
dc.contributor.authorscopusid6701492347-
dc.contributor.authorscopusid7003603506-
dc.description.lastpage166en_US
dc.description.firstpage155en_US
dc.relation.volume50en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.wosWOS:000076443700006-
dc.contributor.daisngid1833931-
dc.contributor.daisngid10897118-
dc.contributor.daisngid25909238-
dc.contributor.daisngid1841045-
dc.contributor.daisngid18788341-
dc.contributor.daisngid749099-
dc.contributor.daisngid1724161-
dc.identifier.investigatorRIDH-1527-2015-
dc.identifier.investigatorRIDNo ID-
dc.description.numberofpages12en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Navarro, D-
dc.contributor.wosstandardWOS:Doreste, H-
dc.contributor.wosstandardWOS:Cabrera, JJ-
dc.contributor.wosstandardWOS:Morales, M-
dc.contributor.wosstandardWOS:Diaz-Chico, JC-
dc.contributor.wosstandardWOS:Diaz-Chico, BN-
dc.date.coverdateOctubre 1998en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.jcr2,287-
dc.description.jcrqQ2-
dc.description.scieSCIE-
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología-
crisitem.author.deptGIR IUIBS: Farmacología Molecular y Traslacional-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptGIR IUIBS: Bioquímica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología-
crisitem.author.deptGIR IUIBS: Bioquímica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología-
crisitem.author.orcid0000-0002-4184-6403-
crisitem.author.orcid0000-0002-0944-990X-
crisitem.author.orcid0000-0002-0944-990X-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameNavarro Bosch, Domingo-
crisitem.author.fullNameCabrera Galván,Juan José-
crisitem.author.fullNameDíaz Chico, Juan Carlos-
crisitem.author.fullNameDíaz Chico, Juan Carlos-
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