Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/48424
DC FieldValueLanguage
dc.contributor.authorBoada, Luis D.en_US
dc.contributor.authorZumbado, Manuelen_US
dc.contributor.authorTorres, Santiagoen_US
dc.contributor.authorLópez, Antonioen_US
dc.contributor.authorDíaz-Chico, Bonifacio N.en_US
dc.contributor.authorCabrera, Juan J.en_US
dc.contributor.authorLuzardo, Octavio P.en_US
dc.contributor.otherZumbado, Manuel-
dc.contributor.otherDominguez-Boada, Luis-
dc.contributor.otherCabrera-Galvan, Juan Jose-
dc.contributor.otherP. Luzardo, Octavio-
dc.date.accessioned2018-11-23T21:40:15Z-
dc.date.available2018-11-23T21:40:15Z-
dc.date.issued1999en_US
dc.identifier.issn0340-5761en_US
dc.identifier.urihttp://hdl.handle.net/10553/48424-
dc.description.abstractStanozolol (ST) is a 17 alpha-alkyl anabolic-androgenic steroid (17 alpha-AAS) often misused by athletes and bodybuilders. The use of anabolic-steroids by sportsmen and teenagers has increased dramatically, thus raising the question about their hepatotoxicity, specially those such as ST which are orally administered. Previously, we have reported diverse in vivo effects exerted by this steroid and published the existence of a highly specific ST-binding site in male rat liver microsomes. The existence of this binding site, the reported hepatic effects exerted in humans, and the very limited information about its potential hepatotoxicity led us to treat adult male rats acutely and chronically with ST and study different parameters that could indicate liver damage: serum levels of transaminases, concentration of monooxygenase enzymes in liver, liver membrane lipid peroxidation products, liver histopathology, and cell cycle/ploidy status of liver cells. In our study, no changes in serum transaminases or lipid peroxidation levels were obtained. However, acute stanozolol treatment significantly decreased the levels of cytochrome P450 (Cyt. P450) and cytochrome b(5) (Cyt. b(5)) during the first 48 h of treatment, while subsequently, at 72 and 96 h, these microsomal enzymes underwent a significant increase in their levels. In sharp contrast with this response to acute treatment, the content of these two enzymes during chronic treatment showed an important decrease. Interestingly, acutely and chronically ST-treated livers showed slight to moderate inflammatory or degenerative lesions in centrilobular hepatocytes. Flow cytometric analysis demonstrated that both acute and chronic ST treatment were capable of increasing the percentage of S-phase fraction (%SPF) of liver cells. These findings taken together clearly show that this steroid is capable of altering the liver capacity for metabolizing xenobiotics and indicate that high doses of ST could exert a proliferative effect on liver cells. Such data should be considered in risk evaluations for this compound.en_US
dc.languageengen_US
dc.relation.ispartofArchives of Toxicologyen_US
dc.sourceArchives of Toxicology[ISSN 0340-5761],v. 73, p. 465-472en_US
dc.subject32 Ciencias médicasen_US
dc.subject3214 Toxicologíaen_US
dc.subject.otherLiver-Microsomesen_US
dc.subject.otherCell-Proliferationen_US
dc.subject.otherEthinyl Estradiolen_US
dc.subject.otherCytochrome-P-450en_US
dc.subject.otherMetabolismen_US
dc.subject.otherLesionsen_US
dc.subject.otherGrowthen_US
dc.titleEvaluation of acute and chronic hepatotoxic effects exerted by anabolic- androgenic steroid stanozolol in adult male ratsen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1007/s002040050636en_US
dc.identifier.scopus0033406253-
dc.identifier.isi000084336200009-
dcterms.isPartOfArchives Of Toxicology-
dcterms.sourceArchives Of Toxicology[ISSN 0340-5761],v. 73 (8-9), p. 465-472-
dc.contributor.authorscopusid6603916807-
dc.contributor.authorscopusid6603459604-
dc.contributor.authorscopusid57192268038-
dc.contributor.authorscopusid57206586568-
dc.contributor.authorscopusid57206586568-
dc.contributor.authorscopusid7003603506-
dc.contributor.authorscopusid6602257053-
dc.contributor.authorscopusid6507534124-
dc.description.lastpage472en_US
dc.description.firstpage465en_US
dc.relation.volume73en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.wosWOS:000084336200009-
dc.contributor.daisngid697526-
dc.contributor.daisngid418822-
dc.contributor.daisngid2770121-
dc.contributor.daisngid6915984-
dc.contributor.daisngid12934188-
dc.contributor.daisngid1724161-
dc.contributor.daisngid1841045-
dc.contributor.daisngid418704-
dc.identifier.investigatorRIDB-4495-2010-
dc.identifier.investigatorRIDL-5577-2015-
dc.identifier.investigatorRIDNo ID-
dc.identifier.investigatorRIDNo ID-
dc.description.numberofpages8en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Boada, LD-
dc.contributor.wosstandardWOS:Zumbado, M-
dc.contributor.wosstandardWOS:Torres, S-
dc.contributor.wosstandardWOS:Lopez, A-
dc.contributor.wosstandardWOS:Diaz-Chico, BN-
dc.contributor.wosstandardWOS:Cabrera, JJ-
dc.contributor.wosstandardWOS:Luzardo, OP-
dc.date.coverdateDiciembre 1999en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.jcr1,683-
dc.description.jcrqQ1-
dc.description.scieSCIE-
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR IUIBS: Medio Ambiente y Salud-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.deptGIR IUIBS: Medio Ambiente y Salud-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.deptGIR IUIBS: Bioquímica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología-
crisitem.author.deptGIR IUIBS: Farmacología Molecular y Traslacional-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptGIR IUIBS: Medio Ambiente y Salud-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.orcid0000-0002-0195-4565-
crisitem.author.orcid0000-0002-1534-7758-
crisitem.author.orcid0000-0002-0944-990X-
crisitem.author.orcid0000-0002-4184-6403-
crisitem.author.orcid0000-0002-4153-3028-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameDomínguez Boada, Luis María-
crisitem.author.fullNameZumbado Peña, Manuel Luis-
crisitem.author.fullNameDíaz Chico, Juan Carlos-
crisitem.author.fullNameCabrera Galván,Juan José-
crisitem.author.fullNamePérez Luzardo, Octavio Luis-
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