Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/48292
DC FieldValueLanguage
dc.contributor.authorMorales, Victoriaen_US
dc.contributor.authorSantana, Pinoen_US
dc.contributor.authorDíaz, Raquelen_US
dc.contributor.authorTabraue, Carlosen_US
dc.contributor.authorGallardo, Germánen_US
dc.contributor.authorLópez Blanco, Félixen_US
dc.contributor.authorHernández, Inmaculadaen_US
dc.contributor.authorFanjul Rodríguez, Luisa Fernandaen_US
dc.contributor.authorRuiz De Galarreta, Carlos M.en_US
dc.contributor.otherTabraue, Carlos-
dc.contributor.otherHernandez Gonzalez, Inmaculada-
dc.contributor.otherSantana-Delgado, Pino-
dc.date.accessioned2018-11-23T20:29:13Z-
dc.date.available2018-11-23T20:29:13Z-
dc.date.issued2003en_US
dc.identifier.issn0013-7227en_US
dc.identifier.urihttp://hdl.handle.net/10553/48292-
dc.description.abstractSystemic or intratesticular release of TNFα and IL1β have been implicated in the reduced testosterone biosynthesis and impaired production of competent spermatozoa found in human patients suffering from sepsis or chronic inflammation. Although in vitro and in vivo studies have demonstrated that TNFα and IL1β intercept the hypothalamic-pituitary testis axis at different levels, the site(s) of action and relative contribution of each cytokine to the overall testicular failure associated to systemic inflammatory processes remains poorly defined. In this study we show that intratesticular delivery of TNFα induced a rapid (4 h) and sustained (up to 24 h) reduction in steroidogenic acute regulatory (StAR) protein expression and testosterone biosynthesis in nonstimulated or human chorionic gonadotropin-treated intact or hypophysectomized rats. Bilateral treatment with cell-permeant short-chain ceramides (C2-cer or C6-cer) reproduced the early (4 h) inhibitory action of TNFα on testosterone biosynthesis and testicular StAR expression. The inhibitory action of C2-cer or C6-cer was not observed in animals treated with inactive analogs (dihydroceramide), phosphorylcholine, sphingosine, or sphoingosine-1P. In sharp contrast to the previously described ability of IL1β to prevent human chorionic gonadotropin-stimulated Leydig cell steroidogenesis in vitro, serum testosterone and testicular StAR protein expression remained unchanged in animals bilaterally injected with this cytokine. These data support the concept that TNFα triggers different effector mechanisms to directly inhibit Leydig cell StAR expression and steroidogenesis, which ultimately contribute to the global reproductive failure associated with chronic inflammation and sepsis.en_US
dc.languageengen_US
dc.relation.ispartofEndocrinology (Philadelphia)en_US
dc.sourceEndocrinology, [ISSN 0013-7227], v. 144 (11), p. 4763-4772en_US
dc.subject230221 Biología molecularen_US
dc.subject2403 Bioquímicaen_US
dc.subject320502 Endocrinologíaen_US
dc.subject.otherCultured Granulosa-Cellsen_US
dc.subject.otherLevels In-Vivoen_US
dc.subject.otherTesticular Macrophagesen_US
dc.subject.otherInjected Interleukin-1-Betaen_US
dc.subject.otherProinflammatory Cytokinesen_US
dc.subject.otherGonadotropin Stimulationen_US
dc.subject.otherSphingosine 1-Phosphateen_US
dc.subject.otherTestosterone Secretionen_US
dc.subject.otherLuteinizing-Hormoneen_US
dc.subject.otherMessenger-Rnaen_US
dc.titleIntratesticular delivery of tumor necrosis factor-alpha and ceramide directly abrogates steroidogenic acute regulatory protein expression and Leydig cell steroidogenesis in adult ratsen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1210/en.2003-0569en_US
dc.identifier.pmid12959973-
dc.identifier.scopus0242636865-
dc.identifier.isi000186178300017-
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dcterms.isPartOfEndocrinology-
dcterms.sourceEndocrinology[ISSN 0013-7227],v. 144 (11), p. 4763-4772-
dc.contributor.authorscopusid7005232305-
dc.contributor.authorscopusid7003526778-
dc.contributor.authorscopusid7201925856-
dc.contributor.authorscopusid57197332997-
dc.contributor.authorscopusid6506833060-
dc.contributor.authorscopusid6603046608-
dc.contributor.authorscopusid7102243115-
dc.contributor.authorscopusid6602478628-
dc.contributor.authorscopusid7102922509-
dc.contributor.authorscopusid57211220417-
dc.contributor.authorscopusid7004158812-
dc.contributor.authorscopusid7003806034-
dc.description.lastpage4772en_US
dc.identifier.issue11-
dc.description.firstpage4763en_US
dc.relation.volume144en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.wosWOS:000186178300017-
dc.contributor.daisngid1664025-
dc.contributor.daisngid31818872-
dc.contributor.daisngid10372875-
dc.contributor.daisngid13600456-
dc.contributor.daisngid4816905-
dc.contributor.daisngid22271832-
dc.contributor.daisngid7801524-
dc.contributor.daisngid2864371-
dc.contributor.daisngid2446020-
dc.contributor.daisngid1127140-
dc.contributor.daisngid1664323-
dc.identifier.investigatorRIDD-7126-2015-
dc.identifier.investigatorRIDK-7776-2014-
dc.identifier.investigatorRIDNo ID-
dc.description.numberofpages10en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Morales, V-
dc.contributor.wosstandardWOS:Santana, P-
dc.contributor.wosstandardWOS:Diaz, R-
dc.contributor.wosstandardWOS:Tabraue, C-
dc.contributor.wosstandardWOS:Gallardo, G-
dc.contributor.wosstandardWOS:Blanco, FL-
dc.contributor.wosstandardWOS:Hernandez, I-
dc.contributor.wosstandardWOS:Fanjul, LF-
dc.contributor.wosstandardWOS:de Galarreta, CMR-
dc.date.coverdateNoviembre 2003en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.jcr5,063-
dc.description.jcrqQ1-
dc.description.scieSCIE-
item.grantfulltextopen-
item.fulltextCon texto completo-
crisitem.author.deptGIR IUIBS: Farmacología Molecular y Traslacional-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Morfología-
crisitem.author.deptGIR IATEXT: Didáctica, Aprendizaje y Motivación en Contextos Específicos-
crisitem.author.deptIU de Análisis y Aplicaciones Textuales-
crisitem.author.deptDepartamento de Didácticas Específicas-
crisitem.author.deptGIR IUIBS: Farmacología Molecular y Traslacional-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología-
crisitem.author.orcid0000-0001-9920-8116-
crisitem.author.orcid0000-0002-1516-1750-
crisitem.author.orcid0000-0003-1167-9787-
crisitem.author.orcid0009-0000-1982-055X-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Análisis y Aplicaciones Textuales-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameTabraue Tarbay, Carlos-
crisitem.author.fullNameGallardo Campos, Germán-
crisitem.author.fullNameLópez Blanco, Félix-
crisitem.author.fullNameFanjul Rodríguez, Luisa Fernanda-
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