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dc.contributor.authorStåhlberg, Ninaen_US
dc.contributor.authorMerino, Roxanaen_US
dc.contributor.authorHernández, Luis Henríquezen_US
dc.contributor.authorFernández-Pérez, Leandroen_US
dc.contributor.authorSandelin, Albinen_US
dc.contributor.authorEngström, Pären_US
dc.contributor.authorTollet-Egnell, Petraen_US
dc.contributor.authorLenhard, Borisen_US
dc.contributor.authorFlores-Morales, Amilcaren_US
dc.description.abstractBackground: Microarray analysis is attractive within the field of endocrine research because regulation of gene expression is a key mechanism whereby hormones exert their actions. Knowledge discovery and testing of hypothesis based on information-rich expression profiles promise to accelerate discovery of physiologically relevant hormonal mechanisms of action. However, most studies so-far concentrate on the analysis of actions of single hormones and few examples exist that attempt to use compilation of different hormone-regulated expression profiles to gain insight into how hormone act to regulate tissue physiology. This report illustrates how a meta-analysis of multiple transcript profiles obtained from a single tissue, the liver, can be used to evaluate relevant hypothesis and discover novel mechanisms of hormonal action. We have evaluated the differential effects of Growth Hormone (GH) and estrogen in the regulation of hepatic gender differentiated gene expression as well as the involvement of sterol regulatory element-binding proteins (SREBPs) in the hepatic actions of GH and thyroid hormone. Results: Little similarity exists between liver transcript profiles regulated by 17-α-ethinylestradiol and those induced by the continuos infusion of bGH. On the other hand, strong correlations were found between both profiles and the female enriched transcript profile. Therefore, estrogens have feminizing effects in male rat liver which are different from those induced by GH. The similarity between bGH and T3 were limited to a small group of genes, most of which are involved in lipogenesis. An in silico promoter analysis of genes rapidly regulated by thyroid hormone predicted the activation of SREBPs by short-term treatment in vivo. It was further demonstrated that proteolytic processing of SREBP1 in the endoplasmic reticulum might contribute to the rapid actions of T3 on these genes. Conclusion: This report illustrates how a meta-analysis of multiple transcript profiles can be used to link knowledge concerning endocrine physiology to hormonally induced changes in gene expression. We conclude that both GH and estrogen are important determinants of gender-related differences in hepatic gene expression. Rapid hepatic thyroid hormone effects affect genes involved in lipogenesis possibly through the induction of SREBP1 proteolytic processing.en_US
dc.relationMecanismos Moleculares y Celulares de Señalización Intracelular en Respuesta A la Hormona de Crecimiento Humana: la Vía Jak (Janus Kinase) Stat (Signal Transducer And Activator Of Transcription) Coen_US
dc.relation.ispartofBMC Physiologyen_US
dc.sourceBMC Physiology[ISSN 1472-6793],v. 5 (Junio 2005)en_US
dc.subject32 Ciencias médicasen_US
dc.subject241010 Fisiología humanaen_US
dc.subject.otherGrowth Hormoneen_US
dc.subject.otherThyroid Hormoneen_US
dc.subject.otherTranscriptional Start Siteen_US
dc.subject.otherGrowth Hormone Treatmenten_US
dc.subject.otherThyroid Hormone Receptoren_US
dc.titleExploring hepatic hormone actions using a compilation of gene expression profilesen_US
dc.investigacionCiencias de la Saluden_US
dc.utils.revisionen_US 2005en_US
item.fulltextCon texto completo- IUIBS: Farmacología Molecular y Traslacional- de Investigaciones Biomédicas y Sanitarias- de Ciencias Clínicas- IUIBS: Farmacología Molecular y Traslacional- de Investigaciones Biomédicas y Sanitarias- de Investigaciones Biomédicas y Sanitarias- de Investigaciones Biomédicas y Sanitarias-ández Pérez, Leandro Fco- Morales,Amilcar-
crisitem.project.principalinvestigatorFernández Pérez, Leandro Fco-
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