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Title: IGM and IGG response to 29-35-kDa Toxoplasma gondii protein fractions in experimentally infected goats
Authors: Conde De Felipe, M. M.
Molina Caballero, José Manuel 
Rodríguez-Ponce, E. 
Ruiz Reyes, Antonio 
González, J. F. 
Keywords: Linked-Immunosorbent-Assay
Ovine Toxoplasmosis
Antigens, et al
Issue Date: 2007
Publisher: 0022-3395
Journal: Journal of Parasitology 
Abstract: The evolution of the Immoral responses of IgG and IgM against 29-35-kDa Toxoplasma gondii fractions from experimentally infected goats were studied and compared by ELISA with the use of whole T. gondii soluble extracts and 29-35-kDa electroeluted proteins as antigens. The IgM response to electroeluted proteins was detected from wk 1 to wk 3 postinfection, showing a similar evolution to that observed when T gondii crude extracts were used as antigens. These results suggest that this group of proteins could be used for a more specific detection of anti-T gondii IgM. In the same way, the IgG response was equivalent in both cases, although when 29-35-kDa T gondii fractions were used as antigens, the level of specific IgGs reached a peak 2 wk before than when T gondii crude extract was used. The detection by ELISA of anti-T. gondii IgM in goats does not seem to be affected by the presence of specific IgG in serum samples when 2935-kDa protein fractions were used as antigens.
ISSN: 0022-3395
DOI: 10.1645/GE-993R.1
Source: Journal of Parasitology[ISSN 0022-3395],v. 93, p. 701-703
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