Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/47919
DC FieldValueLanguage
dc.contributor.authorFernández-Pérez, L.en_US
dc.contributor.authorFlores-Morales, A.en_US
dc.contributor.authorChirino-Godoy, R.en_US
dc.contributor.authorDíaz-Chico, J. C.en_US
dc.contributor.authorDíaz-Chico, B. N.en_US
dc.contributor.otherDiaz-Chico, Juan-
dc.contributor.otherFernandez-Perez, Leandro-
dc.date.accessioned2018-11-23T17:30:22Z-
dc.date.available2018-11-23T17:30:22Z-
dc.date.issued2008en_US
dc.identifier.issn0960-0760en_US
dc.identifier.urihttp://hdl.handle.net/10553/47919-
dc.description.abstractSteroid hormones activate target cells through specific receptors that discriminate among ligands based upon recognition of distinct structural features. For most known steroids, membrane and nuclear receptors co-exist in many target cells. However, while the structure of the nuclear receptors and their function as transcriptional activators of specific target genes is generally well understood, the identity of the membrane receptors remains elusive. Using pharmacological and biochemical approaches, we are beginning to characterize receptors for glucocorticoids and anabolic-androgenic steroids in male rat liver membranes. Male rat liver endoplasmic reticulum contains two steroid binding sites which are functionally related and associated with a 90-134kDa oligomeric protein: (1) the low-affinity glucocorticoid binding site (LAGS), composed at least in part of two peptides (37 and 53 kDa) that bind glucocorticoids and (2) the stanozolol binding protein (STBP), composed at least in part of three peptides (22,31, and 55 kDa) that bind the synthetic androgen stanozolol. These steroid binding proteins have many properties different from those of classical nuclear receptors, with the salient differences being a failure to recognize "classical" ligands for nuclear receptors together with marked differences in biochemical properties and physiological regulation. The mechanism of interaction of glucocorticoids with the LAGS can be clearly distinguished from that with STBP. Moreover, STBP shows an extremely narrow pharmacological profile, being selective for ST and its analog, danazol, among more than 100 steroids and non-steroidal compounds that were assayed, including those that are able to displace glucocorticoids from the LAGS. The level of LAGS activity undergoes dramatic variations following changes from the physiological serum levels of thyroid hormones, glucocorticoids, GH, vitamin A, and E2. However, neither thyroid hormones nor GH have a critical role on STBP activity. The STBP is functionally related to LAGS. We have suggested a novel mechanism for STBP whereby membrane-associated glucocorticoid binding activity is targeted by stanozolol (and 16 beta-hydroxylated stanozolol): stanozolol modulates glucocorticoid activity in the liver through negative allosteric modulation of the LAGS resulting in an effective increase in classical GR-signaling by increasing glucocorticoid availability to the cytosolic GR.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Steroid Biochemistry and Molecular Biologyen_US
dc.sourceJournal of Steroid Biochemistry and Molecular Biology[ISSN 0960-0760],v. 109, p. 336-343en_US
dc.subject32 Ciencias médicasen_US
dc.subject2415 Biología molecularen_US
dc.subject.otherMale-Rat Liveren_US
dc.subject.otherPhotoaffinity-Labeling Identificationen_US
dc.subject.otherMessenger-Ribonucleic-Aciden_US
dc.subject.otherGrowth Factor-Ien_US
dc.subject.otherGene-Expressionen_US
dc.subject.otherPlasma-Membraneen_US
dc.subject.otherDexamethasone-Bindingen_US
dc.subject.otherThyroid-Hormoneen_US
dc.subject.otherEndoplasmic-Reticulumen_US
dc.subject.otherOligomeric Proteinen_US
dc.titleSteroid binding sites in liver membranes: Interplay between glucocorticoids, sex steroids, and pituitary hormonesen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.relation.conference12th International Congress on Hormonal Steroids and Hormones and Cancer-
dc.identifier.doi10.1016/j.jsbmb.2008.03.019en_US
dc.identifier.scopus44149083508-
dc.identifier.isi000257007500023-
dcterms.isPartOfJournal Of Steroid Biochemistry And Molecular Biology-
dcterms.sourceJournal Of Steroid Biochemistry And Molecular Biology[ISSN 0960-0760],v. 109 (3-5), p. 336-343-
dc.contributor.authorscopusid6506777525-
dc.contributor.authorscopusid57203543352-
dc.contributor.authorscopusid6503852944-
dc.contributor.authorscopusid6701492347-
dc.contributor.authorscopusid7003603506-
dc.description.lastpage343en_US
dc.description.firstpage336en_US
dc.relation.volume109en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.wosWOS:000257007500023-
dc.contributor.daisngid795544-
dc.contributor.daisngid617657-
dc.contributor.daisngid22060239-
dc.contributor.daisngid32855587-
dc.contributor.daisngid749099-
dc.contributor.daisngid1724161-
dc.identifier.investigatorRIDH-1527-2015-
dc.identifier.investigatorRIDNo ID-
dc.description.numberofpages8en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Fernandez-Perez, L-
dc.contributor.wosstandardWOS:Flores-Morales, A-
dc.contributor.wosstandardWOS:Chirino-Godoy, R-
dc.contributor.wosstandardWOS:Diaz-Chico, JC-
dc.contributor.wosstandardWOS:Diaz-Chico, BN-
dc.date.coverdateAbril 2008en_US
dc.identifier.conferenceidevents120625-
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.jcr2,827-
dc.description.jcrqQ2-
dc.description.scieSCIE-
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR IUIBS: Farmacología Molecular y Traslacional-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.deptGIR IUIBS: Farmacología Molecular y Traslacional-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptGIR IUIBS: Diabetes y endocrinología aplicada-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptGIR IUIBS: Bioquímica Farmacológica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptGIR IUIBS: Bioquímica Farmacológica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.orcid0000-0001-7802-465X-
crisitem.author.orcid0000-0002-0828-8921-
crisitem.author.orcid0000-0002-0944-990X-
crisitem.author.orcid0000-0002-0944-990X-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameFernández Pérez, Leandro Fco-
crisitem.author.fullNameFlores Morales,Amilcar-
crisitem.author.fullNameChirino Godoy, Ricardo-
crisitem.author.fullNameDíaz Chico, Juan Carlos-
crisitem.author.fullNameDíaz Chico, Juan Carlos-
crisitem.event.eventsstartdate13-09-2006-
crisitem.event.eventsenddate16-09-2006-
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