Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/47911
DC FieldValueLanguage
dc.contributor.authorLee-Montero, I.en_US
dc.contributor.authorNavarro, A.en_US
dc.contributor.authorBorrell, Y.en_US
dc.contributor.authorGarcía-Celdrán, M.en_US
dc.contributor.authorMartín, N.en_US
dc.contributor.authorNegrín-Báez, D.en_US
dc.contributor.authorBlanco, G.en_US
dc.contributor.authorArmero, E.en_US
dc.contributor.authorBerbel, C.en_US
dc.contributor.authorZamorano, M. J.en_US
dc.contributor.authorSánchez, J. J.en_US
dc.contributor.authorEstévez, A.en_US
dc.contributor.authorRamis, G.en_US
dc.contributor.authorManchado, M.en_US
dc.contributor.authorAfonso, J. M.en_US
dc.contributor.otherManchado, Manuel-
dc.contributor.otherRamis, Guillermo-
dc.contributor.otherBorrell Pichs, Yaisel-
dc.contributor.otherZamorano, Maria Jesus-
dc.contributor.otherBlanco-Blanco, Maria Gloria-
dc.contributor.otherEstevez, Alicia-
dc.contributor.otherEstevez, Alicia-
dc.date.accessioned2018-11-23T17:26:11Z-
dc.date.available2018-11-23T17:26:11Z-
dc.date.issued2013en_US
dc.identifier.issn0268-9146en_US
dc.identifier.urihttp://hdl.handle.net/10553/47911-
dc.description.abstractThe high number of multiplex PCRs developed for gilthead seabream (Sparus aurata L.) from many different microsatellite markers does not allow comparison among populations. This highlights the need for developing a reproducible panel of markers, which can be used with safety and reliability by all users. In this study, the first standardised panel of two new microsatellite multiplex PCRs was developed for this species. Primers of 138 specific microsatellites from the genetic linkage map were redesigned and evaluated according to their genetic variability, allele size range and genotyping reliability. A protocol to identify and classify genotyping errors or potential errors was proposed to assess the reliability of each marker. Two new multiplex PCRs from the best assessed markers were designed with 11 markers in each, named SMsa1 and SMsa2 (SuperMultiplex Sparus aurata). Three broodstocks (59, 47 and 98 breeders) from different Spanish companies, and a sample of 80 offspring from each one, were analysed to validate the usefulness of these multiplexes in the parental assignation. It was possible to assign each offspring to a single parent pair (100% success) using the exclusion method with SMsa1 and/or SMsa2. In each genotyped a reference sample (Ref-sa) was used, and its DNA is available on request similar to the kits of bin set to genotype by GENEMAPPER (v.3.7) software (kit-SMsa1 and kit-SMsa2). This will be a robust and effective tool for pedigree analysis or characterisation of populations and will be proposed as an international panel for this species.en_US
dc.languagespaen_US
dc.publisher0268-9146-
dc.relation.ispartofAnimal Geneticsen_US
dc.sourceAnimal Genetics[ISSN 0268-9146],v. 44 (5), p. 533-546en_US
dc.subject.otherPopulation Genetic-Structureen_US
dc.subject.otherCross-Species Amplificationen_US
dc.subject.otherGenotyping Errorsen_US
dc.subject.otherNull Allelesen_US
dc.subject.otherSea Breamen_US
dc.subject.otherPedigree Analysisen_US
dc.subject.otherLinkage Mapen_US
dc.subject.otherParentageen_US
dc.subject.otherMarkersen_US
dc.subject.otherAssignmenten_US
dc.titleDevelopment of the first standardised panel of two new microsatellite multiplex PCRs for gilthead seabream (Sparus aurata L.)en_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1111/age.12037en_US
dc.identifier.scopus84883447087-
dc.identifier.isi000323724300006-
dcterms.isPartOfAnimal Genetics-
dcterms.sourceAnimal Genetics[ISSN 0268-9146],v. 44 (5), p. 533-546-
dc.contributor.authorscopusid55644147300-
dc.contributor.authorscopusid13612121000-
dc.contributor.authorscopusid57212839113-
dc.contributor.authorscopusid6602517453-
dc.contributor.authorscopusid55224003000-
dc.contributor.authorscopusid57190276324-
dc.contributor.authorscopusid55644309600-
dc.contributor.authorscopusid57193934904-
dc.contributor.authorscopusid6602850384-
dc.contributor.authorscopusid55645340300-
dc.contributor.authorscopusid6701451831-
dc.contributor.authorscopusid7403998988-
dc.contributor.authorscopusid56269460100-
dc.contributor.authorscopusid19235779700-
dc.contributor.authorscopusid6603277540-
dc.contributor.authorscopusid57201126472-
dc.description.lastpage546en_US
dc.description.firstpage533en_US
dc.relation.volume44en_US
dc.type2Artículoen_US
dc.identifier.wosWOS:000323724300006-
dc.contributor.daisngid5976729-
dc.contributor.daisngid2095438-
dc.contributor.daisngid946404-
dc.contributor.daisngid4090047-
dc.contributor.daisngid18606437-
dc.contributor.daisngid4127459-
dc.contributor.daisngid1158944-
dc.contributor.daisngid4548804-
dc.contributor.daisngid2963559-
dc.contributor.daisngid7979486-
dc.contributor.daisngid1009633-
dc.contributor.daisngid8078817-
dc.contributor.daisngid291346-
dc.contributor.daisngid280030-
dc.contributor.daisngid285240-
dc.contributor.daisngid1136306-
dc.identifier.investigatorRIDM-8379-2015-
dc.identifier.investigatorRIDL-9021-2014-
dc.identifier.investigatorRIDB-2650-2009-
dc.identifier.investigatorRIDM-5052-2018-
dc.identifier.investigatorRIDNo ID-
dc.identifier.investigatorRIDNo ID-
dc.identifier.investigatorRIDNo ID-
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Lee-Montero, I-
dc.contributor.wosstandardWOS:Navarro, A-
dc.contributor.wosstandardWOS:Borrell, Y-
dc.contributor.wosstandardWOS:Garcia-Celdran, M-
dc.contributor.wosstandardWOS:Martin, N-
dc.contributor.wosstandardWOS:Negrin-Baez, D-
dc.contributor.wosstandardWOS:Blanco, G-
dc.contributor.wosstandardWOS:Armero, E-
dc.contributor.wosstandardWOS:Berbel, C-
dc.contributor.wosstandardWOS:Zamorano, MJ-
dc.contributor.wosstandardWOS:Sanchez, JJ-
dc.contributor.wosstandardWOS:Estevez, A-
dc.contributor.wosstandardWOS:Ramis, G-
dc.contributor.wosstandardWOS:Manchado, M-
dc.contributor.wosstandardWOS:Afonso, JM-
dc.date.coverdateAgosto 2013en_US
dc.identifier.ulpgcen_US
dc.description.sjr1,009
dc.description.jcr2,21
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGrupo de Investigación en Acuicultura-
crisitem.author.deptIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.deptPatología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGrupo de Investigación en Acuicultura-
crisitem.author.deptIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.deptPatología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0003-1569-9152-
crisitem.author.parentorgIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.parentorgIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.fullNameZamorano Serrano, María Jesús-
crisitem.author.fullNameAfonso López, Juan Manuel-
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