Please use this identifier to cite or link to this item:
http://hdl.handle.net/10553/47911
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee-Montero, I. | en_US |
dc.contributor.author | Navarro, A. | en_US |
dc.contributor.author | Borrell, Y. | en_US |
dc.contributor.author | García-Celdrán, M. | en_US |
dc.contributor.author | Martín, N. | en_US |
dc.contributor.author | Negrín-Báez, D. | en_US |
dc.contributor.author | Blanco, G. | en_US |
dc.contributor.author | Armero, E. | en_US |
dc.contributor.author | Berbel, C. | en_US |
dc.contributor.author | Zamorano, M. J. | en_US |
dc.contributor.author | Sánchez, J. J. | en_US |
dc.contributor.author | Estévez, A. | en_US |
dc.contributor.author | Ramis, G. | en_US |
dc.contributor.author | Manchado, M. | en_US |
dc.contributor.author | Afonso, J. M. | en_US |
dc.contributor.other | Manchado, Manuel | - |
dc.contributor.other | Ramis, Guillermo | - |
dc.contributor.other | Borrell Pichs, Yaisel | - |
dc.contributor.other | Zamorano, Maria Jesus | - |
dc.contributor.other | Blanco-Blanco, Maria Gloria | - |
dc.contributor.other | Estevez, Alicia | - |
dc.contributor.other | Estevez, Alicia | - |
dc.date.accessioned | 2018-11-23T17:26:11Z | - |
dc.date.available | 2018-11-23T17:26:11Z | - |
dc.date.issued | 2013 | en_US |
dc.identifier.issn | 0268-9146 | en_US |
dc.identifier.uri | http://hdl.handle.net/10553/47911 | - |
dc.description.abstract | The high number of multiplex PCRs developed for gilthead seabream (Sparus aurata L.) from many different microsatellite markers does not allow comparison among populations. This highlights the need for developing a reproducible panel of markers, which can be used with safety and reliability by all users. In this study, the first standardised panel of two new microsatellite multiplex PCRs was developed for this species. Primers of 138 specific microsatellites from the genetic linkage map were redesigned and evaluated according to their genetic variability, allele size range and genotyping reliability. A protocol to identify and classify genotyping errors or potential errors was proposed to assess the reliability of each marker. Two new multiplex PCRs from the best assessed markers were designed with 11 markers in each, named SMsa1 and SMsa2 (SuperMultiplex Sparus aurata). Three broodstocks (59, 47 and 98 breeders) from different Spanish companies, and a sample of 80 offspring from each one, were analysed to validate the usefulness of these multiplexes in the parental assignation. It was possible to assign each offspring to a single parent pair (100% success) using the exclusion method with SMsa1 and/or SMsa2. In each genotyped a reference sample (Ref-sa) was used, and its DNA is available on request similar to the kits of bin set to genotype by GENEMAPPER (v.3.7) software (kit-SMsa1 and kit-SMsa2). This will be a robust and effective tool for pedigree analysis or characterisation of populations and will be proposed as an international panel for this species. | en_US |
dc.language | spa | en_US |
dc.publisher | 0268-9146 | - |
dc.relation.ispartof | Animal Genetics | en_US |
dc.source | Animal Genetics[ISSN 0268-9146],v. 44 (5), p. 533-546 | en_US |
dc.subject.other | Population Genetic-Structure | en_US |
dc.subject.other | Cross-Species Amplification | en_US |
dc.subject.other | Genotyping Errors | en_US |
dc.subject.other | Null Alleles | en_US |
dc.subject.other | Sea Bream | en_US |
dc.subject.other | Pedigree Analysis | en_US |
dc.subject.other | Linkage Map | en_US |
dc.subject.other | Parentage | en_US |
dc.subject.other | Markers | en_US |
dc.subject.other | Assignment | en_US |
dc.title | Development of the first standardised panel of two new microsatellite multiplex PCRs for gilthead seabream (Sparus aurata L.) | en_US |
dc.type | info:eu-repo/semantics/Article | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1111/age.12037 | en_US |
dc.identifier.scopus | 84883447087 | - |
dc.identifier.isi | 000323724300006 | - |
dcterms.isPartOf | Animal Genetics | - |
dcterms.source | Animal Genetics[ISSN 0268-9146],v. 44 (5), p. 533-546 | - |
dc.contributor.authorscopusid | 55644147300 | - |
dc.contributor.authorscopusid | 13612121000 | - |
dc.contributor.authorscopusid | 57212839113 | - |
dc.contributor.authorscopusid | 6602517453 | - |
dc.contributor.authorscopusid | 55224003000 | - |
dc.contributor.authorscopusid | 57190276324 | - |
dc.contributor.authorscopusid | 55644309600 | - |
dc.contributor.authorscopusid | 57193934904 | - |
dc.contributor.authorscopusid | 6602850384 | - |
dc.contributor.authorscopusid | 55645340300 | - |
dc.contributor.authorscopusid | 6701451831 | - |
dc.contributor.authorscopusid | 7403998988 | - |
dc.contributor.authorscopusid | 56269460100 | - |
dc.contributor.authorscopusid | 19235779700 | - |
dc.contributor.authorscopusid | 6603277540 | - |
dc.contributor.authorscopusid | 57201126472 | - |
dc.description.lastpage | 546 | en_US |
dc.description.firstpage | 533 | en_US |
dc.relation.volume | 44 | en_US |
dc.type2 | Artículo | en_US |
dc.identifier.wos | WOS:000323724300006 | - |
dc.contributor.daisngid | 5976729 | - |
dc.contributor.daisngid | 2095438 | - |
dc.contributor.daisngid | 946404 | - |
dc.contributor.daisngid | 4090047 | - |
dc.contributor.daisngid | 18606437 | - |
dc.contributor.daisngid | 4127459 | - |
dc.contributor.daisngid | 1158944 | - |
dc.contributor.daisngid | 4548804 | - |
dc.contributor.daisngid | 2963559 | - |
dc.contributor.daisngid | 7979486 | - |
dc.contributor.daisngid | 1009633 | - |
dc.contributor.daisngid | 8078817 | - |
dc.contributor.daisngid | 291346 | - |
dc.contributor.daisngid | 280030 | - |
dc.contributor.daisngid | 285240 | - |
dc.contributor.daisngid | 1136306 | - |
dc.identifier.investigatorRID | M-8379-2015 | - |
dc.identifier.investigatorRID | L-9021-2014 | - |
dc.identifier.investigatorRID | B-2650-2009 | - |
dc.identifier.investigatorRID | M-5052-2018 | - |
dc.identifier.investigatorRID | No ID | - |
dc.identifier.investigatorRID | No ID | - |
dc.identifier.investigatorRID | No ID | - |
dc.utils.revision | Sí | en_US |
dc.contributor.wosstandard | WOS:Lee-Montero, I | - |
dc.contributor.wosstandard | WOS:Navarro, A | - |
dc.contributor.wosstandard | WOS:Borrell, Y | - |
dc.contributor.wosstandard | WOS:Garcia-Celdran, M | - |
dc.contributor.wosstandard | WOS:Martin, N | - |
dc.contributor.wosstandard | WOS:Negrin-Baez, D | - |
dc.contributor.wosstandard | WOS:Blanco, G | - |
dc.contributor.wosstandard | WOS:Armero, E | - |
dc.contributor.wosstandard | WOS:Berbel, C | - |
dc.contributor.wosstandard | WOS:Zamorano, MJ | - |
dc.contributor.wosstandard | WOS:Sanchez, JJ | - |
dc.contributor.wosstandard | WOS:Estevez, A | - |
dc.contributor.wosstandard | WOS:Ramis, G | - |
dc.contributor.wosstandard | WOS:Manchado, M | - |
dc.contributor.wosstandard | WOS:Afonso, JM | - |
dc.date.coverdate | Agosto 2013 | en_US |
dc.identifier.ulpgc | Sí | en_US |
dc.description.sjr | 1,009 | |
dc.description.jcr | 2,21 | |
dc.description.sjrq | Q1 | |
dc.description.jcrq | Q1 | |
dc.description.scie | SCIE | |
item.grantfulltext | none | - |
item.fulltext | Sin texto completo | - |
crisitem.author.dept | GIR Grupo de Investigación en Acuicultura | - |
crisitem.author.dept | IU de Investigación en Acuicultura Sostenible y Ec | - |
crisitem.author.dept | Departamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos | - |
crisitem.author.dept | GIR Grupo de Investigación en Acuicultura | - |
crisitem.author.dept | IU de Investigación en Acuicultura Sostenible y Ec | - |
crisitem.author.dept | Departamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos | - |
crisitem.author.orcid | 0000-0003-1569-9152 | - |
crisitem.author.parentorg | IU de Investigación en Acuicultura Sostenible y Ec | - |
crisitem.author.parentorg | IU de Investigación en Acuicultura Sostenible y Ec | - |
crisitem.author.fullName | Zamorano Serrano, María Jesús | - |
crisitem.author.fullName | Afonso López, Juan Manuel | - |
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