Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/47835
DC FieldValueLanguage
dc.contributor.authorHernández Castellano, Lorenzo Enriqueen_US
dc.contributor.authorArgueello, Anastasioen_US
dc.contributor.authorAlmeida, André M.en_US
dc.contributor.authorCastro, Noemien_US
dc.contributor.authorBendixen, Emøkeen_US
dc.contributor.otherCIISA, FMV-
dc.contributor.otherCastro, Noemi-
dc.contributor.otherAlmeida, Andre-
dc.contributor.otherHernandez-Castellano, Lorenzo Enrique-
dc.contributor.otherArguello, Anastasio-
dc.date.accessioned2018-11-23T16:50:02Z-
dc.date.available2018-11-23T16:50:02Z-
dc.date.issued2015en_US
dc.identifier.issn0022-0302en_US
dc.identifier.urihttp://hdl.handle.net/10553/47835-
dc.description.abstractColostrum intake is a key factor for newborn ruminant survival because the placenta does not allow the transfer of immune components. Therefore, newborn ruminants depend entirely on passive immunity transfer from the mother to the neonate, through the suckling of colostrum. Understanding the importance of specific colostrum proteins has gained significant attention in recent years. However, proteomics studies of sheep colostrum and their uptake in neonate lambs has not yet been presented. The aim of this study was to describe the proteomes of sheep colostrum and lamb blood plasma, using sodium dodecyl sulfate-PAGE for protein separation and in-gel digestion, followed by liquid chromatography-tandem mass spectrometry of resulting tryptic peptides for protein identification. An isobaric tag for relative and absolute quantitation (iTRAQ)-based proteomics approach was subsequently used to provide relative quantification of how neonatal plasma protein concentrations change as an effect of colostrum intake. The results of this study describe the presence of 70 proteins in the ovine colostrum proteome. Furthermore, colostrum intake resulted in an increase of 8 proteins with important immune functions in the blood plasma of lambs. Further proteomic studies will be necessary, particularly using the selected reaction monitoring approach, to describe in detail the role of specific colostrum proteins for immune transfer to the neonate.en_US
dc.languagespaen_US
dc.publisher0022-0302
dc.relation.ispartofJournal of Dairy Scienceen_US
dc.sourceJournal Of Dairy Science[ISSN 0022-0302],v. 98 (1), p. 135-147en_US
dc.subject.otherPeptidoglycan Recognition Proteins
dc.subject.otherInnate Immune-Response
dc.subject.otherLow-Abundance Proteins
dc.subject.otherBovine Mammary-Gland
dc.subject.otherMajorera Goat Kids
dc.subject.otherImmunoglobulin-G
dc.subject.otherA-Iv
dc.subject.otherPassive Transfer
dc.subject.otherNewborn Lambs
dc.subject.otherShort-Communication
dc.titleColostrum protein uptake in neonatal lambs examined by descriptive and quantitative liquid chromatography-tandem mass spectrometryen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.3168/jds.2014-8143en_US
dc.identifier.scopus84918840134-
dc.identifier.isi000346622600017-
dcterms.isPartOfJournal Of Dairy Science
dcterms.sourceJournal Of Dairy Science[ISSN 0022-0302],v. 98 (1), p. 135-147
dc.contributor.authorscopusid36682092900-
dc.contributor.authorscopusid6701710018-
dc.contributor.authorscopusid35496077900-
dc.contributor.authorscopusid55945318600-
dc.contributor.authorscopusid57200208399-
dc.contributor.authorscopusid6602145569-
dc.description.lastpage147en_US
dc.description.firstpage135en_US
dc.relation.volume98en_US
dc.type2Artículoen_US
dc.identifier.wosWOS:000346622600017-
dc.contributor.daisngid1033930-
dc.contributor.daisngid298051-
dc.contributor.daisngid493849-
dc.contributor.daisngid330531-
dc.contributor.daisngid22085232-
dc.identifier.investigatorRIDD-2242-2016-
dc.identifier.investigatorRIDF-9621-2016-
dc.identifier.investigatorRIDI-4369-2013-
dc.identifier.investigatorRIDT-1034-2016-
dc.identifier.investigatorRIDB-4493-2010-
dc.contributor.wosstandardWOS:Hernandez-Castellano, LE-
dc.contributor.wosstandardWOS:Arguello, A-
dc.contributor.wosstandardWOS:Almeida, AM-
dc.contributor.wosstandardWOS:Castro, N-
dc.contributor.wosstandardWOS:Bendixen, E-
dc.date.coverdateEnero 2015en_US
dc.identifier.ulpgces
dc.description.sjr1,464
dc.description.jcr2,408
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR Producción y Biotecnología Animal-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptGIR Producción y Biotecnología Animal-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR Producción y Biotecnología Animal-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0003-2729-0434-
crisitem.author.orcid0000-0002-4426-0678-
crisitem.author.orcid0000-0002-3026-2031-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameHernández Castellano, Lorenzo Enrique-
crisitem.author.fullNameArgüello Henríquez, Anastasio-
crisitem.author.fullNameCastro Navarro, Noemí-
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