Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/47258
DC FieldValueLanguage
dc.contributor.authorAcosta, F.en_US
dc.contributor.authorReal Valcárcel, Fernandoen_US
dc.contributor.authorRuiz De Galarreta, C. M.en_US
dc.contributor.authorDíaz, R.en_US
dc.contributor.authorPadilla, D.en_US
dc.contributor.authorEllis, A. E.en_US
dc.date.accessioned2018-11-23T12:05:58Z-
dc.date.available2018-11-23T12:05:58Z-
dc.date.issued2003en_US
dc.identifier.issn1050-4648en_US
dc.identifier.urihttp://hdl.handle.net/10553/47258-
dc.description.abstractVirulent strains of Photobacterium damselae subsp. piscieida (Pap) were grown in media with or without glucose supplementation (to enhance polysaccharide capsule formation) and the bactericidal action of nitric oxide (NO) and peroxynitrites was evaluated in a cell-free assay. Treatment with the NO-donor S-nitroso-acetyl-penicillamine (SNAP) induced a dose-and time-dependent decrease in Pdp survival. This effect was greater for strains grown without glucose supplementation (C forms) than for their counterparts grown with glucose supplementation (C+ forms). Addition of superoxide anion (O-2(-)) generating systems (Xanthine/Xanthine oxidase, glucose/glucose oxidase) to the culture media further enhanced the bactericidal effect of NO. A similar bactericidal effect, with the same pattern of sensitivity, was observed when C+ and C forms of the bacteria were treated with 3-morpholino-sydonimide hydrochloride (SIN-1), a compound which simultaneously generates NO and O-2(-). Addition of superoxide dismutase (SOD) or SOD plus catalase (CAT) did not fully reverse the toxic action of SIN-1 and the bactericidal effect was similar for both C and C+ forms suggesting that while NO alone is sufficient to cause damage in all strains of the pathogen tested, growth in glucose supplemented medium enhanced protection to reactive oxygen intermediates rather than NO. (C) 2003 Elsevier Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisher1050-4648
dc.relation.ispartofFish and Shellfish Immunologyen_US
dc.sourceFish and Shellfish Immunology[ISSN 1050-4648],v. 15, p. 241-248en_US
dc.subject.otherSeabream Sparus-Aurataen_US
dc.subject.otherPasteurella-Piscicidaen_US
dc.subject.otherCapsular Polysaccharideen_US
dc.subject.otherSuperoxide-Dismutaseen_US
dc.subject.otherReactive Oxygenen_US
dc.subject.otherResistanceen_US
dc.subject.otherIronen_US
dc.subject.otherInhibitionen_US
dc.subject.otherExpressionen_US
dc.subject.otherGlucose-6-Phosphate-Dehydrogenaseen_US
dc.titleToxicity of nitric oxide and peroxynitrite to Photobacterium damselae subsp. piscicidaen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/S1050-4648(02)00163-8en_US
dc.identifier.scopus0042072738-
dc.identifier.isi000184769800006-
dc.contributor.authorscopusid56269311600-
dc.contributor.authorscopusid7005397318-
dc.contributor.authorscopusid7003806034-
dc.contributor.authorscopusid7201925856-
dc.contributor.authorscopusid57197332997-
dc.contributor.authorscopusid7005088705-
dc.contributor.authorscopusid7201547148-
dc.description.lastpage248en_US
dc.description.firstpage241en_US
dc.relation.volume15en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.contributor.daisngid878226-
dc.contributor.daisngid29464210-
dc.contributor.daisngid1664323-
dc.contributor.daisngid6796274-
dc.contributor.daisngid1039753-
dc.contributor.daisngid196213-
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Acosta, F-
dc.contributor.wosstandardWOS:Real, F-
dc.contributor.wosstandardWOS:de Galarreta, CMR-
dc.contributor.wosstandardWOS:Diaz, R-
dc.contributor.wosstandardWOS:Padilla, D-
dc.contributor.wosstandardWOS:Ellis, AE-
dc.date.coverdateEnero 2003en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-VETen_US
dc.description.jcr1,57
dc.description.jcrqQ1
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR ECOAQUA: Grupo de Investigación en Acuicultura-
crisitem.author.deptIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR IUSA-ONE HEALTH 2.-Sanidad Animal de la Acuicultura y Especies Silvestres, Enfermedades Infecciosas y Seguridad Alimentaria-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR IUSA-ONE HEALTH 2.-Sanidad Animal de la Acuicultura y Especies Silvestres, Enfermedades Infecciosas y Seguridad Alimentaria-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0002-1098-7529-
crisitem.author.orcid0000-0001-6526-0354-
crisitem.author.orcid0000-0002-6678-5029-
crisitem.author.parentorgIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameAcosta Arbelo, Félix Antonio-
crisitem.author.fullNameReal Valcárcel, Fernando-
crisitem.author.fullNamePadilla Castillo, Daniel Fermín-
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