Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/47233
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dc.contributor.authorRemuzgo-Martínez, Sara-
dc.contributor.authorAranzamendi-Zaldunbide, Maitane-
dc.contributor.authorPilares-Ortega, Lilian-
dc.contributor.authorIcardo, José Manuel-
dc.contributor.authorAcosta, Félix-
dc.contributor.authorMartínez-Martínez, Luis-
dc.contributor.authorRamos-Vivas, José-
dc.date.accessioned2018-11-23T11:54:04Z-
dc.date.available2018-11-23T11:54:04Z-
dc.date.issued2013-
dc.identifier.issn1286-4579-
dc.identifier.urihttp://hdl.handle.net/10553/47233-
dc.description.abstractMacrophages play key roles in host defense by recognizing, engulfing, and killing microorganisms. Understanding the response of macrophages to pathogens may provide insights into host defenses and the tactics used by pathogens to circumvent these defenses. In the present study, we investigated the interaction between a clinical isolate of Serratia liquefaciens and macrophages. S. liquefaciens strain HUMV-3250 triggers a fast and potent cytotoxic effect upon infection. This process requires the presence of live bacteria, adherence, and protein synthesis but not phagocytosis/bacterial internalization. Moreover, cytotoxicity assays, analysis of DNA integrity, immunofluorescence, and confocal, scanning, and time-lapse microscopy revealed that macrophage viability decreased rapidly with time upon challenge, and depends on the MOI used. Treatment of macrophages with caspase-1 inhibitors, or with specific inhibitors of phagocytosis, did not alter the infection outcome. Moreover, human macrophages exhibited similar cytotoxic changes after infection with this strain. Macrophages responded to this cytotoxic strain with a robust pattern of pro-inflammatory gene expression. However, phagocytosis attempts to engulf live bacteria were unsuccessful, and the phagocytes were unable to kill the bacteria. We conclude that macrophage cell death occurs rapidly as a result of necrotic events after close contact with S. liquefaciens. These results likely have important implications for understanding Serratia pathogenesis and host response to infection. (c) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.-
dc.publisher1286-4579-
dc.relation.ispartofMicrobes and Infection-
dc.sourceMicrobes and Infection[ISSN 1286-4579],v. 15, p. 480-490-
dc.subject.otherIii Secretion System-
dc.subject.otherCell-Death-
dc.subject.otherApoptosis-
dc.subject.otherMarcescens-
dc.subject.otherMetalloprotease-
dc.subject.otherInfections-
dc.subject.otherNecrosis-
dc.subject.otherOncosis-
dc.subject.otherSepsis-
dc.titleInteraction of macrophages with a cytotoxic Serratia liquefaciens human isolate-
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1016/j.micinf.2013.03.004-
dc.identifier.scopus84879130933-
dc.identifier.isi000321538700007-
dc.contributor.authorscopusid47861384300-
dc.contributor.authorscopusid55645261000-
dc.contributor.authorscopusid47861373800-
dc.contributor.authorscopusid7004311013-
dc.contributor.authorscopusid56269311600-
dc.contributor.authorscopusid7005458506-
dc.contributor.authorscopusid23493408700-
dc.description.lastpage490-
dc.description.firstpage480-
dc.relation.volume15-
dc.type2Artículoes
dc.contributor.daisngid1031649-
dc.contributor.daisngid13522928-
dc.contributor.daisngid8190150-
dc.contributor.daisngid428306-
dc.contributor.daisngid1422265-
dc.contributor.daisngid67474-
dc.contributor.daisngid1854108-
dc.contributor.wosstandardWOS:Remuzgo-Martinez, S-
dc.contributor.wosstandardWOS:Aranzamendi-Zaldunbide, M-
dc.contributor.wosstandardWOS:Pilares-Ortega, L-
dc.contributor.wosstandardWOS:Icardo, JM-
dc.contributor.wosstandardWOS:Acosta, F-
dc.contributor.wosstandardWOS:Martinez-Martinez, L-
dc.contributor.wosstandardWOS:Ramos-Vivas, J-
dc.date.coverdateJunio 2013-
dc.identifier.ulpgces
dc.description.sjr1,518
dc.description.jcr2,731
dc.description.sjrqQ1
dc.description.jcrqQ2
dc.description.scieSCIE
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR ECOAQUA: Grupo de Investigación en Acuicultura-
crisitem.author.deptIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0002-1098-7529-
crisitem.author.parentorgIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.fullNameAcosta Arbelo, Félix Antonio-
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