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Title: Mitochondrial DNA error prophylaxis: Assessing the causes of errors in the GEP'02-03 proficiency testing trial
Authors: Salas, Antonio
Prieto, Lourdes
Montesino, Marta
Albarrán, Cristina
Arroyo, Eduardo
Paredes-Herrera, Miguel R.
Di Lonardo, Ana Ma
Doutremepuich, Christian
Fernández-Fernández, Isabel
De La Vega, Alberto González
Alves, Cíntia
López, Carlos M.
López-Soto, Manolo
Lorente, José A.
Picornell, Antònia
Espinheira, Rosa M.
Hernández, Alexis
Palacio, Ana Ma
Espinoza, Marta
Yunis, Juan J.
Pérez-Lezaun, Anna
Pestano Brito, José Juan 
Carril, Juan Carlos
Corach, Daniel
Vide, Ma Conceiçao
Álvarez-Iglesias, V.
Pinheiro, M. F.
Whittle, Martín R.
Brehm, Antonio
Gómez, Josefina
UNESCO Clasification: 32 Ciencias médicas
320102 Genética clínica
3203 Medicina forense
Keywords: Isfh Working Group
Collaborative Exercises
International Society
Forensic Genetics, et al
Issue Date: 2005
Journal: Forensic Science International 
Abstract: We report the results of the Spanish and Portuguese working group (GEP) of the International Society for Forensic Genetics (ISFG) Collaborative Exercise 2002-2003 on mitochondrial DNA (mtDNA) analysis. Six different samples were submitted to the participating laboratories: four blood stains (M1-M2-M3-M4), one mixture blood sample (M5), and two hair shaft fragments (M6). Most of the labs reported consensus results for the blood stains, slightly improving the results of previous collaborative exercises. Although hair shaft analysis is still carried out by a small number of laboratories, this analysis yielded a high rate of success. On the contrary, the analysis of the mixture blood stain (M5) yielded a lower rate of success; in spite of this, the whole results on M5 typing demonstrated the suitability of mtDNA analysis in mixture samples. We have found that edition errors are among the most common mistakes reported by the different labs. In addition, we have detected contamination events as well as other minor problems, i.e. lack of standarization in nomenclature for punctual and length heteroplasmies, and indels. In the present edition of the GEP-ISFG exercise we have paid special attention to the visual phylogenetic inspection for detecting common sequencing errors.
ISSN: 0379-0738
DOI: 10.1016/j.forsciint.2004.06.008
Source: Forensic Science International [ISSN 0379-0738],v. 148, p. 191-198
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