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Title: Immunohistochemical Distribution of Keratin Proteins in Feline Tissues
Authors: Martin De Las Mulas Gonzalez-Albo,Juana 
Espinosa De Los Monteros Y Zayas, Antonio 
Gómez Villamandos, José Carlos
Fernández Rodríguez, Antonio Jesús 
Vos, Jan H.
UNESCO Clasification: 240110 Histología animal
Keywords: Intermediate Filament Proteins
Cytokeratin Expression
Myoepithelial Cells
Epithelial Tissues, et al
Issue Date: 1994
Publisher: 0931-184X
Journal: Journal of Veterinary Medicine Series A: Physiology Pathology Clinical Medicine 
Abstract: The immunohistochemical distribution pattern of some keratin intermediate filament proteins has been analysed in a wide range of formalin fixed, paraffin embedded feline tissues using one polyclonal and two monoclonal antibodies raised against human keratins by means of the avidin-biotin-peroxidase complex technique. Only the epithelial and mesothelial cells were stained by the three antibodies, but differences in their corresponding staining pattern were noticed. The staining reaction of the polyclonal antibody raised against human skin keratin was found in both stratified and complex epithelia, while that of the monoclonal antibody which recognizes human keratins 8 + 18 + 19 of the Moll catalogue (NCL-5D3) was restricted to some simple epithelia. The staining reaction of the monoclonal antibody which reacts with human keratins 5 + 8 of the Moll catalogue (RCK-102) covered the widest spectrum of feline epithelial tissues analysed, including stratified, complex and simple epithelia. These staining patterns of feline tissues are basically similar with respect to those of corresponding tissues in other mammalian species, although some differences were also noticed and some obvious epithelial tissues were not stained. This study confirms the broad interspecies cross-reactivity of keratin proteins antibodies and demonstrates their capability to differentiate between various types of feline epithelia and some epithelial compartments.
ISSN: 0931-184X
DOI: 10.1111/j.1439-0442.1994.tb00095.x
Source: Journal of Veterinary Medicine Series A: Physiology Pathology Clinical Medicine [ISSN 0514-7158], v. 41, p. 283-297
Appears in Collections:Reseña
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