Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/46088
Campo DC Valoridioma
dc.contributor.authorSantana, Alfredoen_US
dc.contributor.authorVicente-Salar, Nestoren_US
dc.contributor.authorFreire, Raimundoen_US
dc.contributor.authorReig, Juan A.en_US
dc.contributor.authorRoche, Enriqueen_US
dc.date.accessioned2018-11-23T01:15:52Z-
dc.date.available2018-11-23T01:15:52Z-
dc.date.issued2013en_US
dc.identifier.issn0011-4545en_US
dc.identifier.urihttp://hdl.handle.net/10553/46088-
dc.description.abstractWe have isolated from teratomas a residual cell population reluctant to differentiate in the tumor to ectoderm, mesoderm and endoderm. The main characteristics of this population, compared with undifferentiated R1-cells were: persistent expression of Oct4 gene, modest expression of primordial germ cell markers, karyotype alterations and the capability to form teratomas once transplanted again to immunodeficient mice. The aim of the present report is to further characterize this cell population by studying two aspects that could be related to their teratogenecity: LIF insensitivity and the expression of DNA-damage activated proteins. We have used Western blot and immunocytochemistry to address these questions. Persistent phosphorylation of STAT3 was observed in the absence of leukemia inhibitory factor, explaining the insensitivity to the cytokine compared to R1-embryonic stem cells cultured in vitro. Second, we analyzed by Western-blot the expression level of different proteins involved in DNA damage response. Teratoma-isolated cells presented high expression levels of all assayed proteins, except for Rad9. In addition, cytoimmuno-detection using histone γH2AX antibody indicated the presence of DNA instability linked to double strand breaks. The data shown here, despite being preliminary, could help to explain the teratogenecity of this particular cell population.en_US
dc.languageengen_US
dc.relation.ispartofCytologiaen_US
dc.sourceCytologia [ISSN 0011-4545],v. 78, p. 195-202en_US
dc.subject32 Ciencias médicasen_US
dc.subject241003 Citología humanaen_US
dc.subject.otherCyclin Een_US
dc.subject.otherDNA instabilityen_US
dc.subject.otherγH2AXen_US
dc.subject.otherC-Mycen_US
dc.subject.otherOct4en_US
dc.subject.otherSTAT3en_US
dc.titleLIF insensitivity and expression of proteins activated by DNA damage response in teratoma-isolated cells derived from mouse embryonic stem cellsen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1508/cytologia.78.195en_US
dc.identifier.scopus84888210178-
dc.contributor.authorscopusid55617275900-
dc.contributor.authorscopusid15053628100-
dc.contributor.authorscopusid36518533900-
dc.contributor.authorscopusid7006825116-
dc.contributor.authorscopusid7005472886-
dc.description.lastpage202en_US
dc.identifier.issue2-
dc.description.firstpage195en_US
dc.relation.volume78en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.description.numberofpages8en_US
dc.utils.revisionen_US
dc.date.coverdateJunio 2013en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.sjr0,272
dc.description.jcr0,242
dc.description.sjrqQ4
dc.description.jcrqQ4
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IUIBS: Rendimiento humano, ejercicio físico y salud-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.orcid000-0002-1075-9948-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameSantana Rodríguez, Alfredo-
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