Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/45508
Título: Variable functional recovery and minor cell loss in the ganglion cell layer of the lizard gallotia galloti after optic nerve axotomy
Autores/as: Santos, E.
Romero-Aleman, M. M. 
Monzón-Mayor, M. 
Yanes, C. 
Palabras clave: Tenascin-R
Retinal Projections
Amacrine Cells
Light Reflex
Regeneration, et al.
Fecha de publicación: 2014
Editor/a: 0014-4835
Publicación seriada: Experimental Eye Research 
Resumen: The lizard Gallotia gallori shows spontaneous and slow axon regrowth through a permissive glial scar after optic nerve axotomy. Although much of the expression pattern of glial, neuronal and extracellular matrix markers have been analyzed by our group, an estimation of the cell loss in the ganglion cell layer (GCL) and the degree of visual function recovery remained unresolved. Thus, we performed a series of tests indicative of effective visual function (pupillary light reflex, accommodation, visually elicited behavior) in 18 lizards at 3, 6, 9 and 12 months post-axotomy which were then processed for immunohistochemistry for the neuronal markers SMI-31 (neurofilaments), Tuj1 (beta-III tubulin) and SV2 (synaptic vesicles) at the last timepoint. Separately, cell loss in the GCL was estimated by comparative quantitation of DAPI(+) nuclei in control and 12 months experimental lizards. Additionally, 15 lizards were processed for electron microscopy to monitor relevant ultrastructural changes in the GCL, optic nerve and optic tract throughout regeneration. Hypertrophy of RGCs was persistent, morphology of the regenerated nerves varied from narrow to neuroma-like features and larger regenerated axons underwent remyelination by 9 months. The estimated cell loss in the GCL was 27% and two-third of the animals recovered the pupillary light reflex which involves the pretectum. Strikingly, visually elicited behavior involving the tectum was only restored in two specimens, presumably due to the higher complexity of this pathway. These preliminary results indicate that limited functional regeneration occurs spontaneously in the severely injured visual system of the lacertid G. galloti. (C) 2013 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/10553/45508
ISSN: 0014-4835
DOI: 10.1016/j.exer.2013.09.020
Fuente: Experimental Eye Research[ISSN 0014-4835],v. 118, p. 89-99
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