Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/45329
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dc.contributor.authorFernández-Fernández, Marioen_US
dc.contributor.authorGonzález-Antuña, Anaen_US
dc.contributor.authorRodríguez-González, Pabloen_US
dc.contributor.authorÁlvarez, M. Elena Añónen_US
dc.contributor.authorÁlvarez, Francisco V.en_US
dc.contributor.authorIgnacio García Alonso, J.en_US
dc.date.accessioned2018-11-22T08:59:32Z-
dc.date.available2018-11-22T08:59:32Z-
dc.date.issued2014en_US
dc.identifier.issn0009-8981en_US
dc.identifier.urihttp://hdl.handle.net/10553/45329-
dc.description.abstractThe accurate determination of creatinine in serum is essential for the diagnosis and treatment of kidney diseases. The determination of serum creatinine in clinical laboratories is routinely carried out by the Jaffe method or by enzymatic methods that may suffer from interferences. So, the development of reliable, fast and interference-free routine methods for complex serum samples is required. A fast method using isotope dilution mass spectrometry (IDMS) and gas chromatography mass spectrometry (GC-MS) was developed using minimally (13)C labeled creatinine analogs, multiple linear regression and rapid derivatization of creatinine with acetylacetone in 2 min by using focused microwave technology. The proposed method was validated with the analyses of two Certified Reference Materials (ERM-DA252a and ERM-DA253a) and compared with the Jaffe and enzymatic methods when analyzing real serum samples containing variable levels of bilirubin The proposed method is capable of providing accurate serum creatinine concentrations in less than 45 min from sample arrival to full data treatment and can be an alternative routine procedure for creatinine determinations in complex serum samples.en_US
dc.languageengen_US
dc.relation.ispartofClinica chimica acta (Print)en_US
dc.sourceClinica Chimica Acta [ISSN 0009-8981], v. 431, p. 96-102en_US
dc.subject32 Ciencias médicasen_US
dc.subject2403 Bioquímicaen_US
dc.subject.otherFocused microwavesen_US
dc.subject.otherIsotope dilution mass spectrometryen_US
dc.subject.otherSerum creatinine.en_US
dc.titleDevelopment of an isotope dilution GC-MS procedure for the routine determination of creatinine in complex serum samplesen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.cca.2014.01.011en_US
dc.identifier.scopus84894032511-
dc.contributor.authorscopusid7003830613-
dc.contributor.authorscopusid36058862700-
dc.contributor.authorscopusid6603228633-
dc.contributor.authorscopusid57199759490-
dc.contributor.authorscopusid57196863444-
dc.contributor.authorscopusid15033011500-
dc.description.lastpage102en_US
dc.description.firstpage96en_US
dc.relation.volume431en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.description.numberofpages7en_US
dc.utils.revisionen_US
dc.date.coverdateAbril 2014en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.sjr1,022
dc.description.jcr2,824
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.fullNameGonzález Antuña, Ana-
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