Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/44452
Campo DC Valoridioma
dc.contributor.authorSen, Raquel P.en_US
dc.contributor.authorDelicado, Esmerilda G.en_US
dc.contributor.authorCastro López-Tarruella, Enriqueen_US
dc.contributor.authorMiras‐Portugal, M. Teresaen_US
dc.date.accessioned2018-11-21T23:11:13Z-
dc.date.available2018-11-21T23:11:13Z-
dc.date.issued1993en_US
dc.identifier.issn0022-3042en_US
dc.identifier.urihttp://hdl.handle.net/10553/44452-
dc.description.abstractAdenosine transport in cultured chromaffin cells was inhibited by purinergic P2y‐receptor agonists without significant changes in the affinity constant, the values being between 1 ± 0.4 and 1.6 ± 0.6 μM. The Vmax parameter was modified significantly, being 40 ± 1.0, 26 ± 5.0, 32 ± 3.0, and 22 ± 4.7 pmol/106 cells/min for control, adenosine‐5′‐O‐(2‐thiodiphosphate), 5′‐adenylylimidodiphosphate, and P1,P4‐di(adenosine‐5′‐) tetraphosphate (Ap4A) (100 μM for every effector), respectively. Ap4A, a physiological ligand for P2y receptors in chromaffin cells, showed the highest inhibitory effect (45%). This transport inhibition is explained by an increase in the cytosolic Ca2+ concentration ([Ca2+]i) and the activation of protein kinase C (PKC). Experiments of [Ca2+]i measurement with the fura‐2 technique showed that P2y agonists, as well as bradykinin, were able to increase [Ca2+]i, this effect being independent of the presence of extracellular Ca2+. The peptide bradykinin, determined to be coupled to phosphatidylinositol hydrolysis and internal Ca2+ mobilization in chromaffin cells, exhibited a behavior similar to that of P2y agonists in adenosine transport inhibition (39%). P2y agonists and bradykinin increased PKC activity associated with the membrane fraction (about 50% increase in particulate PKC activity with respect to controls). The present studies suggest that adenosine transport is regulated by P2y‐purinergic receptors mediated via Ca2+ mobilization and PKC activation.en_US
dc.languageengen_US
dc.publisher0022-3042-
dc.relation.ispartofJournal of Neurochemistryen_US
dc.sourceJournal of Neurochemistry [ISSN 0022-3042], v. 60, p. 613-619en_US
dc.subject32 Ciencias médicasen_US
dc.subject.otherAdenosine transporten_US
dc.subject.otherChromaffin cellsen_US
dc.subject.otherDiadenosine tetraphosphatesen_US
dc.subject.otherNucleoside transporten_US
dc.subject.otherP2y agonistsen_US
dc.subject.otherPurinergic receptorsen_US
dc.titleEffect of P<inf>2Y</inf>Agonists on Adenosine Transport in Cultured Chromaffin Cellsen_US
dc.typeinfo:eu-repo/semantics/articlees
dc.typeArticlees
dc.identifier.doi10.1111/j.1471-4159.1993.tb03192.x-
dc.identifier.scopus0027339124-
dc.contributor.authorscopusid6507725384-
dc.contributor.authorscopusid6701879595-
dc.contributor.authorscopusid35095882100-
dc.contributor.authorscopusid24528882700-
dc.description.lastpage619-
dc.description.firstpage613-
dc.relation.volume60-
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.ulpgces
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología-
crisitem.author.fullNameCastro López-Tarruella, Enrique-
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