Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/44132
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dc.contributor.authorSuárez-Bonnet, Alejandroen_US
dc.contributor.authorWillis, Claireen_US
dc.contributor.authorPittaway, Rachelen_US
dc.contributor.authorSmith, Kenen_US
dc.contributor.authorMair, Timen_US
dc.contributor.authorPriestnall, Simon L.en_US
dc.date.accessioned2018-11-21T20:28:22Z-
dc.date.available2018-11-21T20:28:22Z-
dc.date.issued2018en_US
dc.identifier.issn1078-1439en_US
dc.identifier.urihttp://hdl.handle.net/10553/44132-
dc.description.abstractObjectives To evaluate the expression of COX-2, E-cadherin, vimentin, 14-3-3σ, and Phosphatase and tensin homolog (PTEN) tumor-related proteins in equine penile papillomas (ePP) and squamous cell carcinomas (ePSCC), the occurrence of epithelial-mesenchymal transition (EMT) at the invasion front (IF) and compare our findings with current knowledge on human penile squamous cell carcinoma (hPSCC). Material and methods We analyzed, by immunohistochemistry in 45 equine penile proliferative epithelial lesions, the expression of COX-2, E-cadherin, vimentin, 14-3-3σ, and PTEN using monoclonal antibodies. Tumors were histopathologically classified as well-differentiated or poorly differentiated using the IF grading scheme. Semiquantitative analysis was performed to determine down or up-regulation of the proteins and association with histopathological characteristics were statistically investigated using Mann–Whitney U test and/or Spearman's tests. Results COX-2 was neo-expressed in 86.6% of the cases and expression progressively increased from ePP to ePSCC (P = 0.0003) and from well to poorly differentiated (P = 0.033). High COX-2 expression was associated with a high mitotic index (MI) (P = 0.026). In contrast to normal epidermis, ePSCC had very low E-cadherin expression in 64% of the cases (P = 0.0005). Vimentin was neo-expressed in 65% of poorly differentiated ePSCC at the IF indicating EMT. Cytoplasmic 14-3-3σ protein expression was reduced in 42% of the ePSCC and additionally, nuclear expression of 14-3-3σ in neoplastic keratinocytes and in the cytoplasm of stromal fibroblasts at the IF was features only found in ePSCC. PTEN protein showed a tendency to be decreased or lost in ePSCC. Conclusions Our study provides evidence of molecular abnormalities in ePSCC similar to those reported for human PSCC. The occurrence of EMT at the IF is a common event in ePSCC. Naturally occurring ePSCC could serve as a valuable preclinical animal model to explore upcoming therapeutic options for hPSCC.en_US
dc.languageengen_US
dc.relation.ispartofUrologic Oncology: Seminars and Original Investigationsen_US
dc.sourceUrologic Oncology: Seminars and Original Investigations [ISSN 1078-1439], v. 36 (12), p. 532.e9-532.e18en_US
dc.subject3109 Ciencias veterinariasen_US
dc.subject.otherAnimal modelen_US
dc.subject.otherEquineen_US
dc.subject.otherEpithelial–mesenchymal transitionen_US
dc.subject.otherInvasion fronten_US
dc.subject.otherPenile canceren_US
dc.titleMolecular carcinogenesis in equine penile cancer: A potential animal model for human penile canceren_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.urolonc.2018.09.004en_US
dc.identifier.scopus85054036436-
dc.contributor.authorscopusid25636181300-
dc.contributor.authorscopusid57204003880-
dc.contributor.authorscopusid57202027782-
dc.contributor.authorscopusid57203272924-
dc.contributor.authorscopusid7005068471-
dc.contributor.authorscopusid6508041786-
dc.description.lastpage532.e18en_US
dc.description.firstpage532.e9en_US
dc.relation.volume36en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.utils.revisionen_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptDepartamento de Morfología-
crisitem.author.fullNameSuárez Bonnet, Alejandro-
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