Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/43342
Título: Ultrastructural characterization of normal Merkel cells in the dog
Autores/as: Ramírez, Gustavo A.
Rodríguez Guisado, Francisco 
Herráez Thomas, Pedro Manuel 
Castro Alonso, Ayoze 
Andrada Borzollino, Marisa Ana 
Espinosa De Los Monteros Y Zayas, Antonio 
Clasificación UNESCO: 310907 Patología
Palabras clave: Anatomy and histology
Cytology
Dogs
Hard palate
Lip, et al.
Fecha de publicación: 2015
Editor/a: 0959-4493
Publicación seriada: Veterinary Dermatology 
Resumen: Background Involvement of Merkel cells (MKs) in different cutaneous diseases as well as in the growth, differentiation and homeostasis of the skin has been previously documented. Hypothesis/Objectives The aim was to assess the ultrastructural features of MKs in canine skin, including morphometrics, highlighting their similarities with and differences from those described for other mammals. Animals Hard palate, nasal planum, lower lip and whisker pad samples were taken from two healthy young dogs destined for academic purposes. Methods Ultrathin sections of samples fixed in osmium tetroxide and embedded in Epon 812 resin were stained with uranyl acetate and lead citrate and examined using a JEOL JEM 2010 transmission electron microscope. Results Ultrastructural characteristics included the following: (i) arrangement in clusters in the basal layer of the epidermis, oral mucosa and external follicular root sheath; (ii) inconstant link with nerve terminal; (iii) oval (10.27 ± 1.64 μm major axis) cell shape with large lobulated nuclei (5.98 ± 1.16 μm major axis); (iv) spine‐like and thick cytoplasmic processes interdigitating with surrounding keratinocytes; (v) presence of desmosomes in the cell body or at the base of spine‐like processes attaching to neighbouring keratinocytes; and (vi) cytoplasm containing loosely arranged intermediate filaments (10.04 ± 1.17 nm) and numerous dense‐core granules (100.1 ± 17.12 nm) arranged in the basal portion of the cytoplasm. Conclusions and clinical importance This study provides the first complete description of the ultrastructural characteristics of MKs in the dog, enhancing our knowledge of the skin structure in this species and providing a basis for future physiological and pathological studies of the role of these cells in normal and damaged canine tissues.
URI: http://hdl.handle.net/10553/43342
ISSN: 0959-4493
DOI: 10.1111/vde.12230
Fuente: Veterinary Dermatology [ISSN 0959-4493], v. 26(5), p. 328-e69
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