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Title: | 17β-Estradiol transcriptionally represses human insulin receptor gene expression causing cellular insulin resistance | Other Titles: | 17 beta-Estradiol transcriptionally represses human insulin receptor gene expression causing cellular insulin resistance | Authors: | García-Arencibia, Moisés Molero, Susana Dávila, Norma Carmen Carranza, M. Calle, Consuelo |
UNESCO Clasification: | 2302 Bioquímica 320507 Neurología |
Keywords: | 17β-Estradiol Human insulin receptor gene Transcriptional repression Insulin resistance Cell survival, et al |
Issue Date: | 2005 | Publisher: | 0145-2126 | Journal: | Leukemia Research | Abstract: | In this study, we demonstrate that 17β-Estradiol (E2) inhibits human insulin receptor (IR) gene expression in a dose- and time-dependent manner in U-937 human promonocytic cells. Using cells transfected with the −1819 to −271 bp fragment of the human IR promoter (wild type promoter) and treated with E2, we show that this repression is regulated at the transcriptional level. The steroid was also found to diminish the insulin responsiveness of the cells in terms of cell survival, DNA synthesis, glucose transport, and glucose oxidation, this last effect possibly involving reduced phosphatidylinositol 3-kinase (PI3-kinase) activity. These data provide new information on the molecular mechanisms of estrogen-inducing insulin resistance in human cells. | URI: | http://hdl.handle.net/10553/43016 | ISSN: | 0145-2126 | DOI: | 10.1016/j.leukres.2004.05.012 | Source: | Leukemia Research[ISSN 0145-2126],v. 29 (1), p. 79-87 |
Appears in Collections: | Artículos |
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