Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/42460
Campo DC Valoridioma
dc.contributor.authorPérez, D.en_US
dc.contributor.authorMuñoz, M. C.en_US
dc.contributor.authorMolina Caballero, J. M.en_US
dc.contributor.authorMuñoz-Caro, T.en_US
dc.contributor.authorSilva, L.M.R.en_US
dc.contributor.authorTaubert, A.en_US
dc.contributor.authorHermosilla, C.en_US
dc.contributor.authorRuiz Reyes, Antonioen_US
dc.date.accessioned2018-11-15T09:26:24Z-
dc.date.available2018-11-15T09:26:24Z-
dc.date.issued2016en_US
dc.identifier.issn0304-4017en_US
dc.identifier.urihttp://hdl.handle.net/10553/42460-
dc.description.abstractExtracellular trap (ET) formation has been demonstrated as novel effector mechanism against diverse pathogens in polymorphonuclear neutrophils (PMN), eosinophils, mast cells, macrophages and recently also in monocytes. In the current study, we show that E. ninakohlyakimovae triggers the deliverance of monocyte-derived ETs in vitro. Fluorescence illustrations as well as scanning electron microscopy (SEM) analyses showed that monocyte-derived ET formation was rapidly induced upon exposure to viable sporozoites, sporocysts and oocysts of E. ninakohlyakimovae. Classical features of monocyte-released ETs were confirmed by the co-localization of extracellular DNA adorned with myeloperoxidase (MPO) and histones (H3) in parasite-entrapping structures. The treatment of caprine monocyte ET structures with NADPH oxidase inhibitor diphenylene iodondium (DPI) significantly reduced ETosis confirming the essential role of reactive oxygen species (ROS) in monocyte mediated ETs formation. Additionally, co-culture of monocytes with viable sporozoites and soluble oocyst antigen (SOA) induced distinct levels of cytokine and chemokine gene transcription. Thus, the transcription of genes encoding for IL-12 and TNF-α was significantly upregulated after sporozoite encounter. In contrast IL-6 and CCL2 gene transcripts were rather weakly induced by parasites. Conversely, SOA only induced the up-regulation of IL-6 and CCL2 gene transcription, and failed to enhance transcripts of IL-12 and TNF-α in vitro. We here report on monocyte-triggered ETs as novel effector mechanism against E. ninakohlyakimovae. Our results strongly suggest that monocyte-mediated innate immune reactions might play an important role in early host immune reactions against E. ninakohlyakimovae in goats.en_US
dc.languageengen_US
dc.relation.ispartofVeterinary Parasitologyen_US
dc.sourceVeterinary Parasitology[ISSN 0304-4017],v. 227, p. 143-150en_US
dc.subject3109 Ciencias veterinariasen_US
dc.subject.otherChemokinesen_US
dc.subject.otherCytokinesen_US
dc.subject.otherEimeria ninakohlyakimovaeen_US
dc.subject.otherExtracellular trapsen_US
dc.subject.otherInnate immune reactionsen_US
dc.subject.otherMonocyteen_US
dc.titleEimeria ninakohlyakimovae induces NADPH oxidase-dependent monocyte extracellular trap formation and upregulates IL-12 and TNF-α, IL-6 and CCL2 gene transcriptionen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.vetpar.2016.07.028en_US
dc.identifier.scopus84982806710-
dc.identifier.isi000382803600023-
dc.contributor.authorscopusid36096860400-
dc.contributor.authorscopusid57199669332-
dc.contributor.authorscopusid55731962600-
dc.contributor.authorscopusid56182418900-
dc.contributor.authorscopusid55791642600-
dc.contributor.authorscopusid6603706756-
dc.contributor.authorscopusid6602143835-
dc.contributor.authorscopusid57201387360-
dc.description.lastpage150en_US
dc.description.firstpage143en_US
dc.relation.volume227en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.contributor.daisngid3415357-
dc.contributor.daisngid29395753-
dc.contributor.daisngid742292-
dc.contributor.daisngid3345282-
dc.contributor.daisngid2054461-
dc.contributor.daisngid384245-
dc.contributor.daisngid319992-
dc.contributor.daisngid1497623-
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Perez, D-
dc.contributor.wosstandardWOS:Munoz, MC-
dc.contributor.wosstandardWOS:Molina, JM-
dc.contributor.wosstandardWOS:Munoz-Caro, T-
dc.contributor.wosstandardWOS:Silva, LMR-
dc.contributor.wosstandardWOS:Taubert, A-
dc.contributor.wosstandardWOS:Hermosilla, C-
dc.contributor.wosstandardWOS:Ruiz, A-
dc.date.coverdateAgosto 2016en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-VETen_US
dc.description.sjr1,173
dc.description.jcr2,356
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR Parasitología, dermatologia y biopatologia veterinaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR Parasitología, dermatologia y biopatologia veterinaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR Parasitología, dermatologia y biopatologia veterinaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0002-0203-5034-
crisitem.author.orcid0000-0002-8338-2512-
crisitem.author.orcid0000-0003-0668-5496-
crisitem.author.parentorgDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.parentorgDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.parentorgDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.fullNameMuñoz Ojeda, María Del Carmen-
crisitem.author.fullNameMolina Caballero, José Manuel-
crisitem.author.fullNameRuiz Reyes, Antonio-
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