Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/42232
Campo DC Valoridioma
dc.contributor.authorQuintana, Carlosen_US
dc.contributor.authorCabrera, Javieren_US
dc.contributor.authorPerdomo Díaz, Juanen_US
dc.contributor.authorEstevez, Franciscoen_US
dc.contributor.authorLoro, Juan F.en_US
dc.contributor.authorReiter, Russel J.en_US
dc.contributor.authorQuintana, Joseen_US
dc.contributor.otherloro, juan-
dc.contributor.otherEstevez, Francisco-
dc.contributor.otherDiaz, Perdomo-
dc.contributor.otherQuintana, Jose-
dc.date.accessioned2018-10-24T09:06:02Z-
dc.date.available2018-10-24T09:06:02Z-
dc.date.issued2016en_US
dc.identifier.issn0742-3098en_US
dc.identifier.urihttp://hdl.handle.net/10553/42232-
dc.description.abstractMelatonin is an endogenous indoleamine with a wide range of biological functions. In addition to modulating circadian rhythms, it plays important roles in the health as an antioxidant. Melatonin has also the ability to induce apoptosis in cancer cells and to enhance the antitumoral activity of chemotherapeutic agents. In this study, the effect of melatonin on hyperthermia-induced apoptosis was explored using human leukemia cells. The results demonstrate that melatonin greatly improved the cytotoxicity of hyperthermia in U937 cells. The potentiation of cell death was achieved with 1 mmol/L concentrations of the indoleamine but not with concentrations close to physiological levels in blood (1 nmol/L). This effect was associated to an enhancement of the apoptotic response, revealed by an increase in cells with hypodiploid DNA content and activation of multiple caspases (caspase-2, caspase-3, caspase-8, and caspase-9). Melatonin also increased hyperthermia-induced Bid activation as well as translocation of Bax from the cytosol to mitochondria and cytochrome c release. Hyperthermia-provoked apoptosis and potentiation by melatonin were abrogated by a broad-spectrum caspase inhibitor (z-VAD-fmk) as well as by specific inhibitors against caspase-8 or caspase-3. In contrast, blocking of the mitochondrial pathway of apoptosis either with a caspase-9 inhibitor or overexpressing the anti-apoptotic protein Bcl-2 (U937/Bcl-2) reduced the number of apoptotic cells in response to hyperthermia but it was unable to suppress melatonin enhancement. Melatonin appears to modulate the apoptotic response triggered by hyperthermia in a cell type-specific manner as similar results were observed in HL-60 but not in K562 or MOLT-3 cells.en_US
dc.languageengen_US
dc.relationEvaluación de Tdf Como Potencial Fármaco Antitumoral.en_US
dc.relation.ispartofJournal of Pineal Researchen_US
dc.sourceJournal Of Pineal Research[ISSN 0742-3098],v. 61 (3), p. 381-395en_US
dc.subject320106 Dermatologíaen_US
dc.subject.otherApoptosisen_US
dc.subject.otherCaspasesen_US
dc.subject.otherHyperthermiaen_US
dc.subject.otherLeukemiaen_US
dc.subject.otherMelatoninen_US
dc.titleMelatonin enhances hyperthermia-induced apoptotic cell death in human leukemia cellsen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1111/jpi.12356en_US
dc.identifier.scopus84986249674-
dc.identifier.isi000383345900010-
dcterms.isPartOfJournal Of Pineal Research-
dcterms.sourceJournal Of Pineal Research[ISSN 0742-3098],v. 61 (3), p. 381-395-
dc.contributor.authorscopusid57214573072-
dc.contributor.authorscopusid35598975600-
dc.contributor.authorscopusid55750901700-
dc.contributor.authorscopusid7003810011-
dc.contributor.authorscopusid6507389169-
dc.contributor.authorscopusid7402574751-
dc.contributor.authorscopusid8681043500-
dc.description.lastpage395en_US
dc.description.firstpage381en_US
dc.relation.volume61en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.wosWOS:000383345900010-
dc.contributor.daisngid453898-
dc.contributor.daisngid240124-
dc.contributor.daisngid32236859-
dc.contributor.daisngid2333322-
dc.contributor.daisngid384944-
dc.contributor.daisngid3210878-
dc.contributor.daisngid227-
dc.contributor.daisngid128315-
dc.identifier.investigatorRIDL-9319-2014-
dc.identifier.investigatorRIDK-5125-2014-
dc.identifier.investigatorRIDNo ID-
dc.identifier.investigatorRIDNo ID-
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Quintana, C-
dc.contributor.wosstandardWOS:Cabrera, J-
dc.contributor.wosstandardWOS:Perdomo, J-
dc.contributor.wosstandardWOS:Estevez, F-
dc.contributor.wosstandardWOS:Loro, JF-
dc.contributor.wosstandardWOS:Reiter, RJ-
dc.contributor.wosstandardWOS:Quintana, J-
dc.date.coverdateOctubre 2016en_US
dc.identifier.ulpgcen_US
dc.description.sjr2,612
dc.description.jcr10,391
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.project.principalinvestigatorQuintana Aguiar, José Martín-
crisitem.author.deptGIR IUIBS: Bioquímica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología-
crisitem.author.deptGIR IUIBS: Bioquímica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología-
crisitem.author.deptGIR IUIBS: Bioquímica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.deptGIR IUIBS: Bioquímica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología-
crisitem.author.orcid0000-0002-0163-393X-
crisitem.author.orcid0000-0002-9728-2774-
crisitem.author.orcid0000-0002-0517-8209-
crisitem.author.orcid0000-0001-8225-4538-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNamePerdomo Díaz, Juan-
crisitem.author.fullNameEstévez Rosas, Francisco Jesús-
crisitem.author.fullNameLoro Ferrer, Juan Francisco-
crisitem.author.fullNameQuintana Aguiar, José Martín-
Colección:Artículos
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